BMRBj - BMREntryMaster

Found 1 Document (1.142 seconds)

BMRB: 4763

15N relaxation data and model-free parameters for the N-terminal receiver domain of double-mutant (D86N/A89T) unphosphorylated NtrC receiver domain (NtrCr(D86N/A89T))

Authors: Kern, D., Volkman, B.F., Wemmer, D.E.
Assembly: NITROGEN REGULATION PROTEIN
Entity: 1. NITROGEN REGULATION PROTEIN (polymer, Thiol state: all free), 124 monomers, 13652.54 Da Detail
MQRGIVWVVD DDSSIRWVLE RALAGAGLTC TTFENGNEVL AALASKTPDV LLSDIRMPGM DGLALLKQIK QRHPMLPVII MTAHSNLDTA VSAYQQGAFD YLPKPFDIDE AVALVERAIS HYQE
Formula weight: 13652.54 Da
Source organism: Salmonella enterica subsp. enterica serovar Typhimurium
Exptl. method: NMR
Data set: heteronucl_NOEs, heteronucl_T1_relaxation, heteronucl_T2_relaxation
Heteronucl. T₁: 97 T₁ values in 1 lists
Coherence Sz, Field strength (¹H) 750 MHz, Pressure 1 atm, Temperature 298 (±1) K, pH 6.75 (±0.2) Detail
Pressure 1 atm, Temperature 298 (±1) K, pH 6.75 (±0.2)
Experiment name 2D 1H-15N HSQC
Heteronucl. T₂: 194 T₂ values in 2 lists
Coherence S(+,-), Field strength (¹H) 750 MHz, 600 MHz, Pressure 1 atm, Temperature 298 (±1) K, pH 6.75 (±0.2) Detail
Pressure 1 atm, Temperature 298 (±1) K, pH 6.75 (±0.2)
Experiment name 2D 1H-15N HSQC
Heteronucl. NOE: 97 NOE values in 1 lists
Value type relative intensities, Field strength (¹H) 750 MHz, Pressure 1 atm, Temperature 298 (±1) K, pH 6.75 (±0.2) Detail
Pressure 1 atm, Temperature 298 (±1) K, pH 6.75 (±0.2)
Experiment name 2D 1H-15N HSQC
Heteronucl. T₁/T₂: 97 T₁/T₂ values in 1 lists
Field strength (¹H) 750 MHz, Pressure 1 atm, Temperature 298 (±1) K, pH 6.75 (±0.2) Detail
Pressure 1 atm, Temperature 298 (±1) K, pH 6.75 (±0.2)
Release date: 2002-04-10
Citation 1: Two-state allosteric behavior in a single domain signaling protein
Volkman, B.F., Lipson, D., Wemmer, D.E., Kern, D.
Science (2001), 291, 2429-2433
Citation 2: Three-dimensional solution structure of the N-terminal receiver domain of NTRC
Volkman, B.F., Nohaile, M.J., Amy, N.K., Kustu, S., Wemmer, D.E.
Biochemistry (1995), 34, 1413-1424, PubMed 7827089 , DOI: , Abstract

Related entities 1. NITROGEN REGULATION PROTEIN, : 298 entities Detail

Experiments performed 1 experiments Detail

#	Name	Isotope labeling	Type	Concentration
1	Nitrogen regulatory protein C N-terminal receiver domain	[U-99% 15N]		0.9 mM
2	phosphate buffer			50 mM

Keywords conformational rearrangement, dynamics, phosphorylation, receiver domain, signal transduction, two-component system

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Found 1 Document (1.142 seconds)

BMRB: 4763

Related entities 1. NITROGEN REGULATION PROTEIN, : 298 entities Detail

Experiments performed 1 experiments Detail

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