BMRB: 4767

1Ha and 13Ca, 13Cb shifts of apocytochrome b562 at pH 5.1 (temperature 293 K)

Authors

D'Amelio, N., Bonvin, A., Czisch, M., Barker, P., Kaptein, R.

Assembly

apocytochrome b562

Entity

1. apocytochrome b562 (polymer, Thiol state: not present), 106 monomers, 11780.08 Da Detail

ADLEDNMETL NDNLKVIEKA DNAAQVKDAL TKMRAAALDA QKATPPKLED KSPDSPEMKD FRHGFDILVG QIDDALKLAN EGKVKEAQAA AEQLKTTRNA YHQKYR

Formula weight

11780.08 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.1 %, Completeness: 41.0 %, Completeness (bb): 80.4 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C
All	41.0 % (453 of 1104)	21.7 % (139 of 640)	67.7 % (314 of 464)
Backbone	80.4 % (423 of 526)	56.4 % (119 of 211)	96.5 % (304 of 315)
Sidechain	19.2 % (131 of 681)	4.7 % (20 of 429)	44.0 % (111 of 252)
Aromatic	0.0 % (0 of 44)	0.0 % (0 of 22)	0.0 % (0 of 22)
Methyl	24.1 % (27 of 112)	10.7 % (6 of 56)	37.5 % (21 of 56)

1. apocytochrome b562

ADLEDNMETL NDNLKVIEKA DNAAQVKDAL TKMRAAALDA QKATPPKLED KSPDSPEMKD FRHGFDILVG QIDDALKLAN EGKVKEAQAA AEQLKTTRNA YHQKYR

Show sample condition

Sample

Temperature 293 (±1) K, pH 5.1 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	apocytochrome b562	[U-15N; U-13C]		1 mM

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LACS Plot; CA

Referencing offset: 0.45 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	TSP	methyl protons	0.0 ppm	internal	indirect	0.101329
13C	TSP	methyl protons	0.0 ppm	internal	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 0.45 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	TSP	methyl protons	0.0 ppm	internal	indirect	0.101329
13C	TSP	methyl protons	0.0 ppm	internal	indirect	0.2514495

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LACS Plot; HA

Referencing offset: -0.02 ppm, Outliers: 1 Detail

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Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	TSP	methyl protons	0.0 ppm	internal	indirect	0.101329
13C	TSP	methyl protons	0.0 ppm	internal	indirect	0.2514495

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LACS Plot; CO

Referencing offset: -0.36 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	TSP	methyl protons	0.0 ppm	internal	direct	1.0
15N	TSP	methyl protons	0.0 ppm	internal	indirect	0.101329
13C	TSP	methyl protons	0.0 ppm	internal	indirect	0.2514495

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Release date

2002-09-22

Citation

The C terminus of apocytochrome b562 undergoes fast motions and slow exchange among ordered conformations resembling the folded state
D'Amelio, N., Bonvin, A., Czisch, M., Barker, P., Kaptein, R.
Biochemistry (2002), 41, 5505-5514, PubMed 11969411 , DOI: , Abstract

Related entities 1. apocytochrome b562, : 1 : 4 : 1 : 95 : 8 entities Detail

Interaction partners 1. apocytochrome b562, : 16 interactors Detail

Experiments performed 5 experiments Detail

Chemical shift validation 3 contents Detail