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Found 1 Document (1.636 seconds)

BMRB: 4780

1F6U

NMR Structure of Stem-loop SL2 of the HIV-1 Psi RNA Packaging Signal Reveals a Novel A-U-A Base-triple Platform

Authors

Amarasinghe, G.K., De Guzman, R.N., Turner, R.B., Summers, M.F.

Assembly

SL2 stem loop RNA from HIV-1

Entity

1. SL2 stem loop RNA from HIV-1 (polymer, Thiol state: not present), 19 monomers, 6211.684 Da Detail

GGCGACUGGU GAGUACGCC

Formula weight

6211.684 Da

Source organism

Human immunodeficiency virus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 82.4 %, Completeness (bb): 81.8 % Detail

Polymer type: polyribonucleotide

	Total	¹H	¹³C
All	82.4 % (239 of 290)	81.2 % (134 of 165)	84.0 % (105 of 125)
Suger, PO4	81.8 % (171 of 209)	80.7 % (92 of 114)	83.2 % (79 of 95)
Nucleobase	84.0 % (68 of 81)	82.4 % (42 of 51)	86.7 % (26 of 30)
Aromatic	83.1 % (59 of 71)	80.5 % (33 of 41)	86.7 % (26 of 30)

1. HIV-1 SL2 stem loop

GGCGACUGGU GAGUACGCC

Show sample condition

Sample #1

Temperature 298 (±2) K

#	Name	Isotope labeling	Type	Concentration
1	HIV-1 SL2 stem loop			0.6 ~ 1.2 mM

Sample #2

Temperature 298 (±2) K

#	Name	Isotope labeling	Type	Concentration
2	HIV-1 SL2 stem loop	[U-13C; U-15N]		0.8 mM

Release date

2002-04-03

Citation

NMR structure of stem-loop SL2 of the HIV-1 psi RNA packaging signal reveals a novel A-U-A base-triple platform
Amarasinghe, G.K., De Guzman, R.N., Turner, R.B., Summers, M.F.
J. Mol. Biol. (2000), 299, 145-156, PubMed 10860728 , DOI 10.1006/jmbi.2000.3710 , Abstract

Entries sharing articles BMRB: 1 entries Detail

BMRB: 4781 released on 2000-07-09
Title NMR Structure of Stem-loop SL2 of the HIV-1 Psi RNA Packaging Signal Reveals a Novel A-U-A Base-triple Platform

Related entities 1. SL2 stem loop RNA from HIV-1, : 1 : 1 entities Detail

Experiments performed 8 experiments Detail

1 2D NOESY

Instrument

#1 Model Bruker AVANCE DMX (600 MHz)

#2 Model Bruker AVANCE DRX (800 MHz)

Sample #1

Temperature 298 (±2) K

#	Name	Isotope labeling	Type	Concentration
1	HIV-1 SL2 stem loop			0.6 ~ 1.2 mM

Sample #2

Temperature 298 (±2) K

#	Name	Isotope labeling	Type	Concentration
2	HIV-1 SL2 stem loop	[U-13C; U-15N]		0.8 mM

2 2D TOCSY

Instrument

#1 Model Bruker AVANCE DMX (600 MHz)

#2 Model Bruker AVANCE DRX (800 MHz)

Sample #1

Temperature 298 (±2) K

#	Name	Isotope labeling	Type	Concentration
1	HIV-1 SL2 stem loop			0.6 ~ 1.2 mM

Sample #2

Temperature 298 (±2) K

#	Name	Isotope labeling	Type	Concentration
2	HIV-1 SL2 stem loop	[U-13C; U-15N]		0.8 mM

3 2D DQF COSY

Instrument

#1 Model Bruker AVANCE DMX (600 MHz)

#2 Model Bruker AVANCE DRX (800 MHz)

Sample #1

Temperature 298 (±2) K

#	Name	Isotope labeling	Type	Concentration
1	HIV-1 SL2 stem loop			0.6 ~ 1.2 mM

Sample #2

Temperature 298 (±2) K

#	Name	Isotope labeling	Type	Concentration
2	HIV-1 SL2 stem loop	[U-13C; U-15N]		0.8 mM

4 1H-13C HMQC

Instrument

#1 Model Bruker AVANCE DMX (600 MHz)

#2 Model Bruker AVANCE DRX (800 MHz)

Sample #1

Temperature 298 (±2) K

#	Name	Isotope labeling	Type	Concentration
1	HIV-1 SL2 stem loop			0.6 ~ 1.2 mM

Sample #2

Temperature 298 (±2) K

#	Name	Isotope labeling	Type	Concentration
2	HIV-1 SL2 stem loop	[U-13C; U-15N]		0.8 mM

5 1H,1H,13C HMQC-NOESY

Instrument

#1 Model Bruker AVANCE DMX (600 MHz)

#2 Model Bruker AVANCE DRX (800 MHz)

Sample #1

Temperature 298 (±2) K

#	Name	Isotope labeling	Type	Concentration
1	HIV-1 SL2 stem loop			0.6 ~ 1.2 mM

Sample #2

Temperature 298 (±2) K

#	Name	Isotope labeling	Type	Concentration
2	HIV-1 SL2 stem loop	[U-13C; U-15N]		0.8 mM

6 1H,1H,13C HCCH-COSY

Instrument

#1 Model Bruker AVANCE DMX (600 MHz)

#2 Model Bruker AVANCE DRX (800 MHz)

Sample #1

Temperature 298 (±2) K

#	Name	Isotope labeling	Type	Concentration
1	HIV-1 SL2 stem loop			0.6 ~ 1.2 mM

Sample #2

Temperature 298 (±2) K

#	Name	Isotope labeling	Type	Concentration
2	HIV-1 SL2 stem loop	[U-13C; U-15N]		0.8 mM

7 1H,1H,13C HCCH-TOCSY

Instrument

#1 Model Bruker AVANCE DMX (600 MHz)

#2 Model Bruker AVANCE DRX (800 MHz)

Sample #1

Temperature 298 (±2) K

#	Name	Isotope labeling	Type	Concentration
1	HIV-1 SL2 stem loop			0.6 ~ 1.2 mM

Sample #2

Temperature 298 (±2) K

#	Name	Isotope labeling	Type	Concentration
2	HIV-1 SL2 stem loop	[U-13C; U-15N]		0.8 mM

8 1H,1H,15N HSQC-NOESY

Instrument

#1 Model Bruker AVANCE DMX (600 MHz)

#2 Model Bruker AVANCE DRX (800 MHz)

Sample #1

Temperature 298 (±2) K

#	Name	Isotope labeling	Type	Concentration
1	HIV-1 SL2 stem loop			0.6 ~ 1.2 mM

Sample #2

Temperature 298 (±2) K

#	Name	Isotope labeling	Type	Concentration
2	HIV-1 SL2 stem loop	[U-13C; U-15N]		0.8 mM

nullKeywords HIV-1, NMR assingment, Platform motif, RNA, Splice donor site

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Found 1 Document (1.636 seconds)

BMRB: 4780

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Entries sharing articles BMRB: 1 entries Detail

Related entities 1. SL2 stem loop RNA from HIV-1, : 1 : 1 entities Detail

Experiments performed 8 experiments Detail

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