BMRBj - BMREntryMaster

	Total	¹³C	¹⁵N
All	74.5 % (519 of 697)	67.9 % (372 of 548)	98.7 % (147 of 149)
Backbone	97.8 % (491 of 502)	97.3 % (365 of 375)	99.2 % (126 of 127)
Sidechain	43.9 % (137 of 312)	40.3 % (117 of 290)	90.9 % (20 of 22)
Aromatic	13.8 % (9 of 65)	6.8 % (4 of 59)	83.3 % (5 of 6)
Methyl	20.3 % (12 of 59)	20.3 % (12 of 59)

1. hen egg white lysozyme

KVFGRCELAA AMKRHGLDNY RGYSLGNWVC AAKFESNFNT EATNRNTDGS TDYGILQINS RWWCNDGRTP GSRNLCNIPC SALLSSDITA SVNCAKKIVS DGNGMNAWVA WRNRCKGTDV QAWIRGCRL

Show sample condition

Sample #1

Temperature 308 (±1) K, pH 3.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hen egg white lysozyme	[U-95% 13C; U-98% 15N]		1.5 mM

Sample #2

Temperature 308 (±1) K, pH 3.8 (±0.2), Details dimyristoyl-phosphatidlycholine (DMPC) and dihexanoyl-phosphatidylcholine (DHPC), [DMPC]/[DHPC]=2.9, in 10mM phosphate buffer at pH 6.5 (93%/7% H2O/D2O) with a small amount of dioxane for 1H chemical shift referencing

#	Name	Isotope labeling	Concentration
2	hen egg white lysozyme	[U-15N]	0.5 mM
3	DMPC/DHPC		5 %
4	phosphate		10 mM
5	H2O		93 %
6	D2O		7 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.07 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.31 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 50 models in PDB: 1E8L, Strand ID: A Detail

RDC

209 RDC values in 2 lists
Field strength (¹H) 0.0 MHz, Temperature 308 (±1) K, pH 3.8 (±0.2) Detail

Release date

2001-04-30

Citation

A refined solution structure of hen lysozyme determined using residual dipolar coupling data
Schwalbe, H., Grimshaw, S., Spencer, A., Buck, M., Boyd, J., Dobson, C.M., Redfield, C., Smith, L.J.
Protein Sci. (2001), 10, 677-688, PubMed 11274458 , DOI 10.1110/ps.43301 , Abstract

Related entities 1. hen egg white lysozyme, : 1 : 106 : 141 entities Detail

Interaction partners 1. hen egg white lysozyme, : 7 interactors Detail

More details for 7 interactors identified by 6 citations

Experiments performed 12 experiments Detail

1 1H-15N NOESY-HSQC

Instrument

#1 Model GE na (750 MHz) Details home built console

#2 Model GE na (600 MHz) Details home built console

#3 Model GE na (500 MHz) Details home built console

#4 Model Bruker na (800 MHz)

#5 Model Bruker na (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 3.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hen egg white lysozyme	[U-95% 13C; U-98% 15N]		1.5 mM

Sample #2

#	Name	Isotope labeling	Concentration
2	hen egg white lysozyme	[U-15N]	0.5 mM
3	DMPC/DHPC		5 %
4	phosphate		10 mM
5	H2O		93 %
6	D2O		7 %

Sample #3

Temperature 308 (±1) K, pH 3.8 (±0.2), Details dimyristoyl-phosphatidlycholine (DMPC), dihexanoyl-phosphatidylcholine (DHPC) and cetyltrimethylammonium bromide (CTBA). DMPC]:[DHPC]:[CTAB] = 2.9:1.0:0.1, in 10mM phosphate buffer at pH 6.5 (93%/7% H2O/D2O) with a small amount of dioxane for 1H chemical shift referencing

#	Name	Isotope labeling	Concentration
7	hen egg white lysozyme	[U-15N]	0.5 mM
8	DMPC/DHPC/CTAB		7.5 %
9	phosphate		10 mM
10	H2O		93 %
11	D2O		7 %

2 1H-15N TOCSY-HSQC

Instrument

#1 Model GE na (750 MHz) Details home built console

#2 Model GE na (600 MHz) Details home built console

#3 Model GE na (500 MHz) Details home built console

#4 Model Bruker na (800 MHz)

#5 Model Bruker na (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 3.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hen egg white lysozyme	[U-95% 13C; U-98% 15N]		1.5 mM

Sample #2

#	Name	Isotope labeling	Concentration
2	hen egg white lysozyme	[U-15N]	0.5 mM
3	DMPC/DHPC		5 %
4	phosphate		10 mM
5	H2O		93 %
6	D2O		7 %

Sample #3

#	Name	Isotope labeling	Concentration
7	hen egg white lysozyme	[U-15N]	0.5 mM
8	DMPC/DHPC/CTAB		7.5 %
9	phosphate		10 mM
10	H2O		93 %
11	D2O		7 %

3 1H-15N HSQC-NOESY-HSQC

Instrument

#1 Model GE na (750 MHz) Details home built console

#2 Model GE na (600 MHz) Details home built console

#3 Model GE na (500 MHz) Details home built console

#4 Model Bruker na (800 MHz)

#5 Model Bruker na (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 3.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hen egg white lysozyme	[U-95% 13C; U-98% 15N]		1.5 mM

Sample #2

#	Name	Isotope labeling	Concentration
2	hen egg white lysozyme	[U-15N]	0.5 mM
3	DMPC/DHPC		5 %
4	phosphate		10 mM
5	H2O		93 %
6	D2O		7 %

Sample #3

#	Name	Isotope labeling	Concentration
7	hen egg white lysozyme	[U-15N]	0.5 mM
8	DMPC/DHPC/CTAB		7.5 %
9	phosphate		10 mM
10	H2O		93 %
11	D2O		7 %

4 HNCO

Instrument

#1 Model GE na (750 MHz) Details home built console

#2 Model GE na (600 MHz) Details home built console

#3 Model GE na (500 MHz) Details home built console

#4 Model Bruker na (800 MHz)

#5 Model Bruker na (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 3.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hen egg white lysozyme	[U-95% 13C; U-98% 15N]		1.5 mM

Sample #2

#	Name	Isotope labeling	Concentration
2	hen egg white lysozyme	[U-15N]	0.5 mM
3	DMPC/DHPC		5 %
4	phosphate		10 mM
5	H2O		93 %
6	D2O		7 %

Sample #3

#	Name	Isotope labeling	Concentration
7	hen egg white lysozyme	[U-15N]	0.5 mM
8	DMPC/DHPC/CTAB		7.5 %
9	phosphate		10 mM
10	H2O		93 %
11	D2O		7 %

5 HNCA

Instrument

#1 Model GE na (750 MHz) Details home built console

#2 Model GE na (600 MHz) Details home built console

#3 Model GE na (500 MHz) Details home built console

#4 Model Bruker na (800 MHz)

#5 Model Bruker na (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 3.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hen egg white lysozyme	[U-95% 13C; U-98% 15N]		1.5 mM

Sample #2

#	Name	Isotope labeling	Concentration
2	hen egg white lysozyme	[U-15N]	0.5 mM
3	DMPC/DHPC		5 %
4	phosphate		10 mM
5	H2O		93 %
6	D2O		7 %

Sample #3

#	Name	Isotope labeling	Concentration
7	hen egg white lysozyme	[U-15N]	0.5 mM
8	DMPC/DHPC/CTAB		7.5 %
9	phosphate		10 mM
10	H2O		93 %
11	D2O		7 %

6 CBCACONH

Instrument

#1 Model GE na (750 MHz) Details home built console

#2 Model GE na (600 MHz) Details home built console

#3 Model GE na (500 MHz) Details home built console

#4 Model Bruker na (800 MHz)

#5 Model Bruker na (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 3.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hen egg white lysozyme	[U-95% 13C; U-98% 15N]		1.5 mM

Sample #2

#	Name	Isotope labeling	Concentration
2	hen egg white lysozyme	[U-15N]	0.5 mM
3	DMPC/DHPC		5 %
4	phosphate		10 mM
5	H2O		93 %
6	D2O		7 %

Sample #3

#	Name	Isotope labeling	Concentration
7	hen egg white lysozyme	[U-15N]	0.5 mM
8	DMPC/DHPC/CTAB		7.5 %
9	phosphate		10 mM
10	H2O		93 %
11	D2O		7 %

7 CBCANH

Instrument

#1 Model GE na (750 MHz) Details home built console

#2 Model GE na (600 MHz) Details home built console

#3 Model GE na (500 MHz) Details home built console

#4 Model Bruker na (800 MHz)

#5 Model Bruker na (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 3.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hen egg white lysozyme	[U-95% 13C; U-98% 15N]		1.5 mM

Sample #2

#	Name	Isotope labeling	Concentration
2	hen egg white lysozyme	[U-15N]	0.5 mM
3	DMPC/DHPC		5 %
4	phosphate		10 mM
5	H2O		93 %
6	D2O		7 %

Sample #3

#	Name	Isotope labeling	Concentration
7	hen egg white lysozyme	[U-15N]	0.5 mM
8	DMPC/DHPC/CTAB		7.5 %
9	phosphate		10 mM
10	H2O		93 %
11	D2O		7 %

8 HCCH-TOCSY

Instrument

#1 Model GE na (750 MHz) Details home built console

#2 Model GE na (600 MHz) Details home built console

#3 Model GE na (500 MHz) Details home built console

#4 Model Bruker na (800 MHz)

#5 Model Bruker na (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 3.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hen egg white lysozyme	[U-95% 13C; U-98% 15N]		1.5 mM

Sample #2

#	Name	Isotope labeling	Concentration
2	hen egg white lysozyme	[U-15N]	0.5 mM
3	DMPC/DHPC		5 %
4	phosphate		10 mM
5	H2O		93 %
6	D2O		7 %

Sample #3

#	Name	Isotope labeling	Concentration
7	hen egg white lysozyme	[U-15N]	0.5 mM
8	DMPC/DHPC/CTAB		7.5 %
9	phosphate		10 mM
10	H2O		93 %
11	D2O		7 %

9 HCCH-NOESY

Instrument

#1 Model GE na (750 MHz) Details home built console

#2 Model GE na (600 MHz) Details home built console

#3 Model GE na (500 MHz) Details home built console

#4 Model Bruker na (800 MHz)

#5 Model Bruker na (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 3.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hen egg white lysozyme	[U-95% 13C; U-98% 15N]		1.5 mM

Sample #2

#	Name	Isotope labeling	Concentration
2	hen egg white lysozyme	[U-15N]	0.5 mM
3	DMPC/DHPC		5 %
4	phosphate		10 mM
5	H2O		93 %
6	D2O		7 %

Sample #3

#	Name	Isotope labeling	Concentration
7	hen egg white lysozyme	[U-15N]	0.5 mM
8	DMPC/DHPC/CTAB		7.5 %
9	phosphate		10 mM
10	H2O		93 %
11	D2O		7 %

10 SOFT HCCH-COSY

Instrument

#1 Model GE na (750 MHz) Details home built console

#2 Model GE na (600 MHz) Details home built console

#3 Model GE na (500 MHz) Details home built console

#4 Model Bruker na (800 MHz)

#5 Model Bruker na (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 3.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hen egg white lysozyme	[U-95% 13C; U-98% 15N]		1.5 mM

Sample #2

#	Name	Isotope labeling	Concentration
2	hen egg white lysozyme	[U-15N]	0.5 mM
3	DMPC/DHPC		5 %
4	phosphate		10 mM
5	H2O		93 %
6	D2O		7 %

Sample #3

#	Name	Isotope labeling	Concentration
7	hen egg white lysozyme	[U-15N]	0.5 mM
8	DMPC/DHPC/CTAB		7.5 %
9	phosphate		10 mM
10	H2O		93 %
11	D2O		7 %

11 SOFT HCCH-E.COSY

Instrument

#1 Model GE na (750 MHz) Details home built console

#2 Model GE na (600 MHz) Details home built console

#3 Model GE na (500 MHz) Details home built console

#4 Model Bruker na (800 MHz)

#5 Model Bruker na (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 3.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hen egg white lysozyme	[U-95% 13C; U-98% 15N]		1.5 mM

Sample #2

#	Name	Isotope labeling	Concentration
2	hen egg white lysozyme	[U-15N]	0.5 mM
3	DMPC/DHPC		5 %
4	phosphate		10 mM
5	H2O		93 %
6	D2O		7 %

Sample #3

#	Name	Isotope labeling	Concentration
7	hen egg white lysozyme	[U-15N]	0.5 mM
8	DMPC/DHPC/CTAB		7.5 %
9	phosphate		10 mM
10	H2O		93 %
11	D2O		7 %

12 HMQCJ

Instrument

#1 Model GE na (750 MHz) Details home built console

#2 Model GE na (600 MHz) Details home built console

#3 Model GE na (500 MHz) Details home built console

#4 Model Bruker na (800 MHz)

#5 Model Bruker na (600 MHz)

Sample #1

Temperature 308 (±1) K, pH 3.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hen egg white lysozyme	[U-95% 13C; U-98% 15N]		1.5 mM

Sample #2

#	Name	Isotope labeling	Concentration
2	hen egg white lysozyme	[U-15N]	0.5 mM
3	DMPC/DHPC		5 %
4	phosphate		10 mM
5	H2O		93 %
6	D2O		7 %

Sample #3

#	Name	Isotope labeling	Concentration
7	hen egg white lysozyme	[U-15N]	0.5 mM
8	DMPC/DHPC/CTAB		7.5 %
9	phosphate		10 mM
10	H2O		93 %
11	D2O		7 %

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts, RDC exptl data	bmr4831_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4831_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	511		100.0 (chain: 1, length: 129)
1	Chemical shift references	chemical_shift_reference	OK	2		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 129, Sequence coverage: 100.0%
- Total number of assignments
  - ¹³C chemical shifts: 364
  - ¹⁵N chemical shifts: 147
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	548	364	66.4
¹⁵N chemical shifts	160	147	91.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	258	254	98.4
¹⁵N chemical shifts	127	126	99.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	290	110	37.9
¹⁵N chemical shifts	33	21	63.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	61	12	19.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	59	0	0.0
¹⁵N chemical shifts	6	6	100.0

Keywords Lysozyme

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Found 1 Document (1.9 seconds)

BMRB: 4831

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. hen egg white lysozyme, : 1 : 106 : 141 entities Detail

Interaction partners 1. hen egg white lysozyme, : 7 interactors Detail

Experiments performed 12 experiments Detail

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