NMR Mapping and Secondary Structure Determination of the Major Acetylcholine Receptor alpha-Subunit Determinant Interacting with alpha-Bungarotoxin
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 1:CYS3:SG | 1:CYS23:SG |
2 | disulfide | sing | 1:CYS29:SG | 1:CYS33:SG |
3 | disulfide | sing | 1:CYS48:SG | 1:CYS59:SG |
4 | disulfide | sing | 1:CYS60:SG | 1:CYS65:SG |
5 | disulfide | sing | 2:CYS13:SG | 2:CYS14:SG |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 55.0 % (318 of 578) | 55.0 % (318 of 578) |
Backbone | 75.1 % (145 of 193) | 75.1 % (145 of 193) |
Sidechain | 44.9 % (173 of 385) | 44.9 % (173 of 385) |
Aromatic | 2.0 % (1 of 49) | 2.0 % (1 of 49) |
Methyl | 74.4 % (29 of 39) | 74.4 % (29 of 39) |
1. alpha-bungarotoxin
IVCHTTATSP ISAVTCPPGE NLCYRKMWCD AFCSSRGKVV ELGCAATCPS KKPYEEVTCC STDKCNPHPK RQPG2. acetylcholine receptor peptide
EERGWKHWVY YTCCPDTPYL DITEETemperature 310 (±0) K, pH 4 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | alpha-bungarotoxin | 2 mM | ||
2 | acetylcholine receptor peptide | 2 mM |
Polymer type: polypeptide(L)
Total | 1H | |
---|---|---|
All | 36.9 % (426 of 1156) | 36.9 % (426 of 1156) |
Backbone | 50.0 % (193 of 386) | 50.0 % (193 of 386) |
Sidechain | 30.3 % (233 of 770) | 30.3 % (233 of 770) |
Aromatic | 1.0 % (1 of 98) | 1.0 % (1 of 98) |
Methyl | 47.4 % (37 of 78) | 47.4 % (37 of 78) |
1. alpha-bungarotoxin
IVCHTTATSP ISAVTCPPGE NLCYRKMWCD AFCSSRGKVV ELGCAATCPS KKPYEEVTCC STDKCNPHPK RQPG2. acetylcholine receptor peptide
EERGWKHWVY YTCCPDTPYL DITEETemperature 310 (±0) K, pH 4 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | alpha-bungarotoxin | 2 mM | ||
2 | acetylcholine receptor peptide | 2 mM |