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Conformational Changes in the Isolated N-terminal Domain of 5-Enolpyruvylshikimate-3-phosphate Synthase upon Shikimate-3-phosphate Binding
Authors
Stauffer, M., Young, J., Helms, G., Evans, J.N.S.
Assembly
N-terminal domain of 5-enolpyruvyl shikimate-3-phosphate synthase
Entity
1. EPSP synthase (polymer, Thiol state: all free), 240 monomers, 25626.11 Da Detail

MESLTLQPIA RVDGTINLPG SKSVSNRALL LAALAHGKTV LTNLLDSDDV RHMLNALTAL GVSYTLSADR TRCEIIGNGG PLHAEGALEL FLGNAGTAMR PLAAALCLGS NDIVLTGEPR MKERPIGHLV DALRLGGAKI TYLEQENYPP LRLQGGFTGG NVDVDGSVSS QFLTALLMTA PLAPEDTVIR IKGDLVSKPY IDITLNLMKT FGVEIENQHY QQFVVKGGQS YQSPGTYLVE


2. shikimate-3-phosphate (non-polymer), 1 monomers, 254.131 Da
Total weight
25880.24 Da
Max. entity weight
25626.11 Da
Source organism
Escherichia coli
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.9 %, Completeness: 86.7 %, Completeness (bb): 92.7 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All86.7 % (2331 of 2690)87.4 % (1203 of 1377)86.4 % (911 of 1054)83.8 % (217 of 259)
Backbone92.7 % (1289 of 1391)94.0 % (440 of 468)91.1 % (633 of 695)94.7 % (216 of 228)
Sidechain82.0 % (1241 of 1514)83.9 % (763 of 909)83.1 % (477 of 574) 3.2 % (1 of 31)
Aromatic 9.5 % (12 of 126)14.3 % (9 of 63) 4.8 % (3 of 63)
Methyl98.5 % (325 of 330)98.2 % (162 of 165)98.8 % (163 of 165)

1. EPSP synthase

MESLTLQPIA RVDGTINLPG SKSVSNRALL LAALAHGKTV LTNLLDSDDV RHMLNALTAL GVSYTLSADR TRCEIIGNGG PLHAEGALEL FLGNAGTAMR PLAAALCLGS NDIVLTGEPR MKERPIGHLV DALRLGGAKI TYLEQENYPP LRLQGGFTGG NVDVDGSVSS QFLTALLMTA PLAPEDTVIR IKGDLVSKPY IDITLNLMKT FGVEIENQHY QQFVVKGGQS YQSPGTYLVE

Sample

Temperature 295 (±1) K


#NameIsotope labelingTypeConcentration
1EPSP synthase[U-15N; U-13C; U70%-2H]1.0 mM
2shikimate-3-phosphate1.5 mM
3Tris buffer50 mM
4DTT1 mM
5NaN33 mM
6pepstatin0.0 ~ 0.0 uM
7leupeptin0.0 ~ 0.0 uM

LACS Plot; CA
Referencing offset: 0.33 ppm, Outliers: 6 Detail
LACS Plot; CB
Referencing offset: 0.33 ppm, Outliers: 6 Detail
LACS Plot; HA
Referencing offset: -0.07 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.47 ppm, Outliers: 2 Detail
Release date
2001-11-13
Citation
Sequential assignments of the isolated N-terminal domain of 5-enolpyruvylshikimate-3-phosphate synthase
Stauffer, M.E., Young, J.K., Helms, G.L., Evans, J.N.S.
J. Biomol. NMR (2001), 20, 387-388, PubMed 11563562 ,
Entries sharing articles BMRB: 1 entries Detail
  BMRB: 4854 released on 2001-11-13
    Title Chemical shift assignments for the isolated N-terminal domain of EPSP synthase
Related entities 1. EPSP synthase, : 1 : 8 : 1 : 17 : 121 entities Detail
Interaction partners 1. EPSP synthase, : 2 interactors Detail
Keywords NMR, EPSP synthase, shikimate-3-phosphate, MurA