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Chemical shift assignments for the isolated N-terminal domain of EPSP synthase
Authors
Stauffer, M., Young, J., Helms, G., Evans, J.N.S.
Assembly
N-terminal domain of 5-enolpyruvyl shikimate-3-phosphate synthase
Entity
1. N-terminal domain of 5-enolpyruvyl shikimate-3-phosphate synthase (polymer, Thiol state: all free), 240 monomers, 25626.11 Da Detail

MESLTLQPIA RVDGTINLPG SKSVSNRALL LAALAHGKTV LTNLLDSDDV RHMLNALTAL GVSYTLSADR TRCEIIGNGG PLHAEGALEL FLGNAGTAMR PLAAALCLGS NDIVLTGEPR MKERPIGHLV DALRLGGAKI TYLEQENYPP LRLQGGFTGG NVDVDGSVSS QFLTALLMTA PLAPEDTVIR IKGDLVSKPY IDITLNLMKT FGVEIENQHY QQFVVKGGQS YQSPGTYLVE


Formula weight
25626.11 Da
Source organism
Escherichia coli
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.3 %, Completeness: 82.1 %, Completeness (bb): 87.9 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All82.1 % (2209 of 2690)81.6 % (1124 of 1377)84.0 % (885 of 1054)77.2 % (200 of 259)
Backbone87.9 % (1222 of 1391)87.2 % (408 of 468)88.3 % (614 of 695)87.7 % (200 of 228)
Sidechain77.6 % (1175 of 1514)78.4 % (713 of 909)80.5 % (462 of 574) 0.0 % (0 of 31)
Aromatic 6.3 % (8 of 126) 6.3 % (4 of 63) 6.3 % (4 of 63)
Methyl95.2 % (314 of 330)94.5 % (156 of 165)95.8 % (158 of 165)

1. N-terminal EPSP synthase

MESLTLQPIA RVDGTINLPG SKSVSNRALL LAALAHGKTV LTNLLDSDDV RHMLNALTAL GVSYTLSADR TRCEIIGNGG PLHAEGALEL FLGNAGTAMR PLAAALCLGS NDIVLTGEPR MKERPIGHLV DALRLGGAKI TYLEQENYPP LRLQGGFTGG NVDVDGSVSS QFLTALLMTA PLAPEDTVIR IKGDLVSKPY IDITLNLMKT FGVEIENQHY QQFVVKGGQS YQSPGTYLVE

Sample

Temperature 295 (±1) K


#NameIsotope labelingTypeConcentration
1N-terminal EPSP synthase[U-15N; U-13C; U-70% 2H]1.0 mM
2Tris50 mM
3DTT1 mM

LACS Plot; CA
Referencing offset: -0.1 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: -0.1 ppm, Outliers: 3 Detail
LACS Plot; HA
Referencing offset: -0.07 ppm, Outliers: 3 Detail
LACS Plot; CO
Referencing offset: 0.29 ppm, Outliers: 7 Detail
Release date
2001-11-13
Citation
Sequential assignments of the isolated N-terminal domain of 5-enolpyruvylshikimate-3-phosphate synthase
Stauffer, M.E., Young, J.K., Helms, G.L., Evans, J.N.S.
J. Biomol. NMR (2001), 20, 387-388, PubMed 11563562 ,
Related entities 1. N-terminal domain of 5-enolpyruvyl shikimate-3-phosphate synthase, : 1 : 8 : 1 : 17 : 121 entities Detail
Interaction partners 1. N-terminal domain of 5-enolpyruvyl shikimate-3-phosphate synthase, : 2 interactors Detail
Experiments performed 7 experiments Detail
Keywords NMR, EPSP synthase, shikimate-3-phosphate, MurA