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BMRB: 4857

1FR0

Solution structure and dynamic character of the histidine-containing phosphotransfer domain of anaerobic sensor kinase ArcB from Escherichia coli

Authors

Ikegami, T., Okada, T., Ohki, I., Hirayama, J., Mizuno, T., Shirakawa, M.

Assembly

HPt-ArcB

Entity

1. HPt-ArcB (polymer, Thiol state: not present), 125 monomers, 13996.80 Da Detail

TTEENSKSEA LLDIPMLEQY LELVGPKLIT DGLAVFEKMM PGYVSVLESN LTAQDKKGIV EEGHKIKGAA GSVGLRHLQQ LGQQIQSPDL PAWEDNVGEW IEEMKEEWRH DVEVLKAWVA KATKK

Formula weight

13996.8 Da

Source organism

Escherichia coli

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 98.4 %, Completeness: 93.2 %, Completeness (bb): 98.0 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	93.2 % (1373 of 1473)	95.1 % (731 of 769)	90.2 % (514 of 570)	95.5 % (128 of 134)
Backbone	98.0 % (725 of 740)	97.6 % (249 of 255)	98.4 % (359 of 365)	97.5 % (117 of 120)
Sidechain	89.7 % (761 of 848)	93.8 % (482 of 514)	83.7 % (268 of 320)	78.6 % (11 of 14)
Aromatic	51.2 % (44 of 86)	93.0 % (40 of 43)	0.0 % (0 of 39)	100.0 % (4 of 4)
Methyl	95.9 % (142 of 148)	95.9 % (71 of 74)	95.9 % (71 of 74)

1. Histidine-containing phosphotransfer domain of E. coli anaerobic sensor kinase ArcB

TTEENSKSEA LLDIPMLEQY LELVGPKLIT DGLAVFEKMM PGYVSVLESN LTAQDKKGIV EEGHKIKGAA GSVGLRHLQQ LGQQIQSPDL PAWEDNVGEW IEEMKEEWRH DVEVLKAWVA KATKK

Show sample condition

Sample

Temperature 310 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Histidine-containing phosphotransfer domain of E. coli anaerobic sensor kinase ArcB	[U-99% 13C; U-99% 15N]		1.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	0.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.39 ppm, Outliers: 3 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	0.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.39 ppm, Outliers: 3 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	0.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: -0.08 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	external	indirect	0.2514495
¹H	DSS	methyl protons	external	direct	0.0
¹⁵N	DSS	methyl protons	external	indirect	0.1013291

Referencing offset: 0.47 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 30 models in PDB: 1FR0, Strand ID: A Detail

Coupling constant

96 J values in 1 lists
Temperature 310 (±1) K, pH 6.5 (±0.2) Detail

Release date

2015-09-01

Citation

Solution structure and dynamic character of the histidine-containing phosphotransfer domain of anaerobic sensor kinase ArcB from Escherichia coli
Ikegami, T., Okada, T., Ohki, I., Hirayama, J., Mizuno, T., Shirakawa, M.
Biochemistry (2001), 40, 375-386, PubMed 11148031 , DOI: , Abstract

Related entities 1. HPt-ArcB, : 1 : 5 : 1 : 3 entities Detail

Interaction partners 1. HPt-ArcB, : 10 interactors Detail

More details for 10 interactors identified by 5 citations

Experiments performed 15 experiments Detail

1 HNCO