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1H NMR Study on the Binding of Pin1 Trp-trp domain with Phosphothreonine Peptides
Authors
Wintjens, R., Wieruszeski, J., Drobecq, H., Rousselot-Pailley, P., Lippens, G., Landrieu, I.
Assembly
Pin1 WW domain
Entity
1. Pin1 WW domain (polymer, Thiol state: not present), 39 monomers, 4455.842 Da Detail

KLPPGWEKRM SRSSGRVYYF NHITNASQWE RPSGNSSSG


Formula weight
4455.842 Da
Source organism
Homo sapiens
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 99.6 %, Completeness (bb): 98.7 % Detail

Polymer type: polypeptide(L)

Total1H
All99.6 % (242 of 243)99.6 % (242 of 243)
Backbone98.7 % (78 of 79)98.7 % (78 of 79)
Sidechain100.0 % (164 of 164)100.0 % (164 of 164)
Aromatic100.0 % (27 of 27)100.0 % (27 of 27)
Methyl100.0 % (8 of 8)100.0 % (8 of 8)

1. Pin1 WW domain

KLPPGWEKRM SRSSGRVYYF NHITNASQWE RPSGNSSSG

Sample

Temperature 285 (±1) K


#NameIsotope labelingTypeConcentration
1Pin1 WW domain1.0 mM

Release date
2001-07-10
Citation
1H NMR study on the binding of Pin1 Trp-Trp domain with phosphothreonine peptides
Wintjens, R., Wieruszeski, J., Drobecq, H., Rousselot-Pailley, P., Buee, L., Lippens, G., Landrieu, I.
J. Biol. Chem. (2001), 276, 25150-25156, PubMed 11313338 , DOI 10.1074/jbc.M010327200 ,
Related entities 1. Pin1 WW domain, : 1 : 12 : 1 : 50 : 212 entities Detail
Interaction partners 1. Pin1 WW domain, : 141 interactors Detail
Experiments performed 2 experiments Detail
Chemical shift validation 3 contents Detail