BMRBj - BMREntryMaster

Found 1 Document (0.885 seconds)

BMRB: 4885

1HEI

Backbone 1H, 15N and 13C Resonance Assignments of the NTPase Subdomain of the Hepatitis C Virus NS3 RNA Helicase

Authors

Liu, D., Wyss, D.F.

Assembly

HCV NS3 RNA helicase

Entity

1. HCV NS3 RNA helicase (polymer, Thiol state: not reported), 144 monomers, 14603.27 Da Detail

SPVFTDNSSP PAVPQSFQVA HLHAPTGSGK STKVPAAYAA QGYKVLVLNP SVAATLGFGA YMSKAHGVDP NIRTGVRTIT TGSPITYSTY GKFLADGGCS GGAYDIIICD ECHSTDATSI LGIGTVLDQA ETAGARLVVL ATAT

Formula weight

14603.27 Da

Source organism

Hepatitis C virus subtype 1a

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.0 %, Completeness: 44.5 %, Completeness (bb): 73.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	44.5 % (669 of 1505)	23.1 % (175 of 757)	61.2 % (371 of 606)	86.6 % (123 of 142)
Backbone	73.8 % (624 of 846)	46.4 % (137 of 295)	87.5 % (364 of 416)	91.1 % (123 of 135)
Sidechain	20.2 % (159 of 787)	8.2 % (38 of 462)	38.1 % (121 of 318)	0.0 % (0 of 7)
Aromatic	0.0 % (0 of 104)	0.0 % (0 of 52)	0.0 % (0 of 52)
Methyl	16.1 % (30 of 186)	10.8 % (10 of 93)	21.5 % (20 of 93)

1. HCV helicase

SPVFTDNSSP PAVPQSFQVA HLHAPTGSGK STKVPAAYAA QGYKVLVLNP SVAATLGFGA YMSKAHGVDP NIRTGVRTIT TGSPITYSTY GKFLADGGCS GGAYDIIICD ECHSTDATSI LGIGTVLDQA ETAGARLVVL ATAT

Show sample condition

Sample

Temperature 293 (±0.5) K, pH 7.4 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	HCV helicase	[U-15N; U-13C; U-2H]		0.075 ~ 0.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	external	direct	0.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.85 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	external	direct	0.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.85 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	TSP	methyl protons	external	direct	0.0
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

Release date

2001-11-13

Citation

Backbone 1H, 15N and 13C resonance assignments of the NTPase subdomain of the hepatitis C virus NS3 RNA helicase
Liu, D., Wyss, D.
J. Biomol. NMR (2001), 19, 283-284, PubMed 11330818 , Abstract

Related entities 1. HCV NS3 RNA helicase, : 1 : 6 : 21 : 1 : 164 entities Detail

Interaction partners 1. HCV NS3 RNA helicase, : 10 interactors Detail

More details for 10 interactors identified by 7 citations

Experiments performed 7 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr4885_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr4885_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_1	OK	594		89.6 (chain: 1, length: 144)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 144, Sequence coverage: 89.6%
- Total number of assignments
  - ¹³C chemical shifts: 356
  - ¹⁵N chemical shifts: 119
  - ¹H chemical shifts: 119
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	606	356	58.7
¹⁵N chemical shifts	145	119	82.1
¹H chemical shifts	757	119	15.7

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	288	244	84.7
¹⁵N chemical shifts	135	119	88.1
¹H chemical shifts	295	119	40.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	318	112	35.2
¹⁵N chemical shifts	10	0	0.0
¹H chemical shifts	462	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	94	19	20.2
¹H chemical shifts	94	0	0.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	52	0	0.0
¹H chemical shifts	52	0	0.0

Keywords HCV, NMR, helicase

Search

Query history

Resources

Search for primary databases

Search for subsidiary databases

Display

Hits per page

Statistics

Query Changes will take effect after "OK" button is selected.

Maximum hits

Default conjunction operator

Omni-box

Search filter

Sequence search (BLAST)

E-value

Query sequence coverage

Miscellaneous

The number of previewed publications

Completeness of assigned chemical shift

Sequence chart about top aligned regions

Sequence chart about interacting regions

Hit context with highlight

Link destinations for BMRB, PDB and EMDB

Found 1 Document (0.885 seconds)

BMRB: 4885

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. HCV NS3 RNA helicase, : 1 : 6 : 21 : 1 : 164 entities Detail

Interaction partners 1. HCV NS3 RNA helicase, : 10 interactors Detail

Experiments performed 7 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail