Backbone 1H and 15N Chemical Shift Assignment of c-Src SH3 in high salt solution
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 30.9 % (151 of 488) | 19.8 % (81 of 410) | 89.7 % (70 of 78) |
Backbone | 65.3 % (143 of 219) | 49.0 % (73 of 149) | 100.0 % (70 of 70) |
Sidechain | 3.0 % (8 of 269) | 3.1 % (8 of 261) | 0.0 % (0 of 8) |
Aromatic | 0.0 % (0 of 44) | 0.0 % (0 of 42) | 0.0 % (0 of 2) |
Methyl | 0.0 % (0 of 39) | 0.0 % (0 of 39) |
1. Src Homology 3 domain
AGGVTTFVAL YDYESRTETD LSFKKGERLQ IVNNTEGDWW LAHSLTTGQT GYIPSNYVAP SDSIQAEGIH RDTemperature 298 (±1) K, pH 6.5 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Src Homology 3 domain | [U-N15] | 1 mM | |
2 | sodium phosphate | 50 mM | ||
3 | sodium sulphate | 200 mM | ||
4 | DTT | 10 mM | ||
5 | pepstatin | 5 mg/mL | ||
6 | EDTA | 50 uM | ||
7 | benzamidine | 10 ug/mL | ||
8 | chloramphenicol | 50 uM |