BMRB: 4889

Backbone 1H and 15N Chemical Shift Assignment of c-Src SH3 complexed with RLP2 ligand in high salt solution

Authors

Wang, C., Pawley, N.H., Nicholson, L.K.

Assembly

c-Src SH3 RLP2 peptide complex

Entity

1. Src Homology 3 domain (polymer, Thiol state: not present), 72 monomers, 7979.576 Da Detail

AGGVTTFVAL YDYESRTETD LSFKKGERLQ IVNNTEGDWW LAHSLTTGQT GYIPSNYVAP SDSIQAEGIH RD

2. RLP2 (polymer, Thiol state: not present), 9 monomers, 1082.298 Da Detail

RALPPLPRY

Total weight

9061.874 Da

Max. entity weight

7979.576 Da

Source organism

Gallus gallus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.4 %, Completeness: 25.8 %, Completeness (bb): 59.2 % Detail

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Polymer type: polypeptide(L)

	Total	1H	15N
All	25.8 % (144 of 558)	15.6 % (74 of 474)	83.3 % (70 of 84)
Backbone	59.2 % (142 of 240)	43.9 % (72 of 164)	92.1 % (70 of 76)
Sidechain	0.6 % (2 of 318)	0.6 % (2 of 310)	0.0 % (0 of 8)
Aromatic	0.0 % (0 of 48)	0.0 % (0 of 46)	0.0 % (0 of 2)
Methyl	2.3 % (1 of 44)	2.3 % (1 of 44)

1. Src Homology 3 domain

AGGVTTFVAL YDYESRTETD LSFKKGERLQ IVNNTEGDWW LAHSLTTGQT GYIPSNYVAP SDSIQAEGIH RD

2. RLP2

RALPPLPRY

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Src Homology 3 domain	[U-15N]		1 mM
2	RLP2			4 eq
3	sodium phosphate			50 mM
4	sodium sulphate			200 mM
5	DTT			10 mM
6	pepstatin			5 mg/mL
7	EDTA			50 uM
8	benzamidine			10 ug/mL
9	chloramphenicol			50 uM

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Release date

2002-01-22

Citation

The role of backbone motions in ligand binding to the c-Src SH3 domain
Wang, C., Pawley, N.H., Nicholson, L.K.
J. Mol. Biol. (2001), 313, 873-887, PubMed 11697910 , DOI 10.1006/jmbi.2001.5083 , Abstract

Entries sharing articles BMRB: 1 entries Detail

Related entities 1. Src Homology 3 domain, : 7 : 157 entities Detail

Experiments performed 3 experiments Detail

Chemical shift validation 4 contents Detail