BMRBj - BMREntryMaster

	Total	¹H	¹³C	¹⁵N
All	58.7 % (595 of 1013)	61.3 % (322 of 525)	54.0 % (207 of 383)	62.9 % (66 of 105)
Backbone	57.6 % (312 of 542)	66.5 % (123 of 185)	48.1 % (129 of 268)	67.4 % (60 of 89)
Sidechain	61.2 % (341 of 557)	58.5 % (199 of 340)	67.7 % (136 of 201)	37.5 % (6 of 16)
Aromatic	72.4 % (42 of 58)	72.4 % (21 of 29)	70.4 % (19 of 27)	100.0 % (2 of 2)
Methyl	64.4 % (58 of 90)	64.4 % (29 of 45)	64.4 % (29 of 45)

1. Survival of Motor Neurons protein

GAMKKNTAAS LQQWKVGDKC SAIWSEDGCI YPATIASIDF KRETCVVVYT GYGNREEQNL SDLLSPICEV ANNIEQNAQE NENESQVSTD E

Show sample condition

Sample #1

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
1	Survival of Motor Neurons protein	[U-100% 15N]	1.0 mM
2	sodium chloride		20 mM
3	phosphate buffer		30 mM
4	H2O		95 %
5	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
6	Survival of Motor Neurons protein	[U-100% 13C; U-100% 15N]	1.0 mM
7	sodium chloride		20 mM
8	phosphate buffer		30 mM
9	H2O		95 %
10	D2O		5 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.81 ppm	internal	direct	0.0
¹⁵N	DSS	protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 4 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.81 ppm	internal	direct	0.0
¹⁵N	DSS	protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: 0.16 ppm, Outliers: 4 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
¹³C	DSS	protons	0.0 ppm	null	indirect	0.2514495
¹H	H2O	protons	4.81 ppm	internal	direct	0.0
¹⁵N	DSS	protons	0.0 ppm	null	indirect	0.1013291

Referencing offset: -0.09 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 10 models in PDB: 1G5V, Strand ID: A Detail

Release date

2001-05-02

Citation 1

SMN tudor domain structure and its interaction with the Sm proteins
Selenko, P., Sprangers, R., Stier, G., Buehler, D., Fischer, U., Sattler, M.
Nat. Struct. Biol. (2001), 8, 27-31, PubMed 11135666 , DOI 10.1038/83014 , Abstract

Show more 3 citaions

Citation 2

NMRPipe: a multidimensional spectral processing system based on UNIX pipes
Delaglio, F., Grzesiek, S., Vuister, G.W., Zhu, G., Pfeifer, J., Bax, A.
J. Biomol. NMR (1995), 6, 277-293, PubMed 8520220 , DOI 10.1007/bf00197809 , Abstract

Citation 3

The program XEASY for computer-supported NMR spectral analysis of biological macromolecules
Bartels, C., Xia, T., Billeter, M., Guntert, P., Wuthrich, K.
J. Biomol. NMR (1995), 6, 1-10, PubMed , DOI:

Citation 4

Crystallography & NMR system: A new software suite for macromolecular structure determination
Brunger, A.T., Adams, P.D., Clore, G.M., DeLano, W.L., Gros, P., Grosse-Kunstleve, R.W., Jiang, J.S., Kuszewski, J., Nilges, M., Pannu, N.S., Read, R.J., Rice, L.M., Simonson, T., Warren, G.L.
Acta Crystallogr. D. Biol. Crystallogr. (1998), 54, 905-921, PubMed 9757107 , DOI 10.1107/s0907444998003254 , Abstract

Hide non-primary 3 citaions

Related entities 1. SMN tudor domain, : 1 : 16 entities Detail

Interaction partners 1. SMN tudor domain, : 161 interactors Detail

More details for 161 interactors identified by 41 citations

Experiments performed 9 experiments Detail

1 HNCA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
1	Survival of Motor Neurons protein	[U-100% 15N]	1.0 mM
2	sodium chloride		20 mM
3	phosphate buffer		30 mM
4	H2O		95 %
5	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
6	Survival of Motor Neurons protein	[U-100% 13C; U-100% 15N]	1.0 mM
7	sodium chloride		20 mM
8	phosphate buffer		30 mM
9	H2O		95 %
10	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
11	Survival of Motor Neurons protein	[U-100% 13C; U-100% 15N]	1.0 mM
12	sodium chloride		20 mM
13	phosphate buffer		30 mM
14	D2O		100 %

2 HN(CO)CA

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
1	Survival of Motor Neurons protein	[U-100% 15N]	1.0 mM
2	sodium chloride		20 mM
3	phosphate buffer		30 mM
4	H2O		95 %
5	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
6	Survival of Motor Neurons protein	[U-100% 13C; U-100% 15N]	1.0 mM
7	sodium chloride		20 mM
8	phosphate buffer		30 mM
9	H2O		95 %
10	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
11	Survival of Motor Neurons protein	[U-100% 13C; U-100% 15N]	1.0 mM
12	sodium chloride		20 mM
13	phosphate buffer		30 mM
14	D2O		100 %

3 CBCANH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
1	Survival of Motor Neurons protein	[U-100% 15N]	1.0 mM
2	sodium chloride		20 mM
3	phosphate buffer		30 mM
4	H2O		95 %
5	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
6	Survival of Motor Neurons protein	[U-100% 13C; U-100% 15N]	1.0 mM
7	sodium chloride		20 mM
8	phosphate buffer		30 mM
9	H2O		95 %
10	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
11	Survival of Motor Neurons protein	[U-100% 13C; U-100% 15N]	1.0 mM
12	sodium chloride		20 mM
13	phosphate buffer		30 mM
14	D2O		100 %

4 CBCA(CO)NH

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
1	Survival of Motor Neurons protein	[U-100% 15N]	1.0 mM
2	sodium chloride		20 mM
3	phosphate buffer		30 mM
4	H2O		95 %
5	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
6	Survival of Motor Neurons protein	[U-100% 13C; U-100% 15N]	1.0 mM
7	sodium chloride		20 mM
8	phosphate buffer		30 mM
9	H2O		95 %
10	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
11	Survival of Motor Neurons protein	[U-100% 13C; U-100% 15N]	1.0 mM
12	sodium chloride		20 mM
13	phosphate buffer		30 mM
14	D2O		100 %

5 H(C)CH TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
1	Survival of Motor Neurons protein	[U-100% 15N]	1.0 mM
2	sodium chloride		20 mM
3	phosphate buffer		30 mM
4	H2O		95 %
5	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
6	Survival of Motor Neurons protein	[U-100% 13C; U-100% 15N]	1.0 mM
7	sodium chloride		20 mM
8	phosphate buffer		30 mM
9	H2O		95 %
10	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
11	Survival of Motor Neurons protein	[U-100% 13C; U-100% 15N]	1.0 mM
12	sodium chloride		20 mM
13	phosphate buffer		30 mM
14	D2O		100 %

6 CCH TOCSY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
1	Survival of Motor Neurons protein	[U-100% 15N]	1.0 mM
2	sodium chloride		20 mM
3	phosphate buffer		30 mM
4	H2O		95 %
5	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
6	Survival of Motor Neurons protein	[U-100% 13C; U-100% 15N]	1.0 mM
7	sodium chloride		20 mM
8	phosphate buffer		30 mM
9	H2O		95 %
10	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
11	Survival of Motor Neurons protein	[U-100% 13C; U-100% 15N]	1.0 mM
12	sodium chloride		20 mM
13	phosphate buffer		30 mM
14	D2O		100 %

7 15N edited NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
1	Survival of Motor Neurons protein	[U-100% 15N]	1.0 mM
2	sodium chloride		20 mM
3	phosphate buffer		30 mM
4	H2O		95 %
5	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
6	Survival of Motor Neurons protein	[U-100% 13C; U-100% 15N]	1.0 mM
7	sodium chloride		20 mM
8	phosphate buffer		30 mM
9	H2O		95 %
10	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
11	Survival of Motor Neurons protein	[U-100% 13C; U-100% 15N]	1.0 mM
12	sodium chloride		20 mM
13	phosphate buffer		30 mM
14	D2O		100 %

8 13C edited NOESY

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
1	Survival of Motor Neurons protein	[U-100% 15N]	1.0 mM
2	sodium chloride		20 mM
3	phosphate buffer		30 mM
4	H2O		95 %
5	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
6	Survival of Motor Neurons protein	[U-100% 13C; U-100% 15N]	1.0 mM
7	sodium chloride		20 mM
8	phosphate buffer		30 mM
9	H2O		95 %
10	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
11	Survival of Motor Neurons protein	[U-100% 13C; U-100% 15N]	1.0 mM
12	sodium chloride		20 mM
13	phosphate buffer		30 mM
14	D2O		100 %

9 IPAP (dipolar couplings)

Instrument

#1 Model Bruker DRX (500 MHz)

#2 Model Bruker DRX (600 MHz)

Sample #1

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
1	Survival of Motor Neurons protein	[U-100% 15N]	1.0 mM
2	sodium chloride		20 mM
3	phosphate buffer		30 mM
4	H2O		95 %
5	D2O		5 %

Sample #2

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
6	Survival of Motor Neurons protein	[U-100% 13C; U-100% 15N]	1.0 mM
7	sodium chloride		20 mM
8	phosphate buffer		30 mM
9	H2O		95 %
10	D2O		5 %

Sample #3

Pressure 1 atm, Temperature 295 (±2) K, pH 6.3 (±0)

#	Name	Isotope labeling	Concentration
11	Survival of Motor Neurons protein	[U-100% 13C; U-100% 15N]	1.0 mM
12	sodium chloride		20 mM
13	phosphate buffer		30 mM
14	D2O		100 %

nullKeywords SMA, Sm interacting protein, SMN, splicing, software, spectrum analysis, Structure analysis, Structure calculation

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Found 1 Document (4.409 seconds)

BMRB: 4899

Sample #1

Sample #2

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. SMN tudor domain, : 1 : 16 entities Detail

Interaction partners 1. SMN tudor domain, : 161 interactors Detail

Experiments performed 9 experiments Detail

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