BMRB: 4916

RECEPTOR-BOUND CONFORMATION OF PACAP21

Authors

Inooka, H., Ohtaki, T., Kitahara, O., Ikegami, T., Endo, S., Kitada, C., Ogi, K., Onda, H., Fujino, M., Shirakawa, M.

Assembly

PITUITARY ADENYLATE CYCLASE ACTIVATING POLYPEPTIDE WITH N-TERMINAL 21 RESIDUES WITH AN AMIDATED C-TERMINUS

Entity

1. Human peptide hormone (polymer, Thiol state: not present), 22 monomers, 2515.824 Da Detail

HSDGIFTDSY SRYRKQMAVK KX

2. G-protein coupled receptor (GPCR) (polymer), na Da

Formula weight

2515.824 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Iterative relaxation matrix analysis (IRMA) and simulated annealing (SA)

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 95.5 %, Completeness: 97.1 %, Completeness (bb): 95.3 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	97.1 % (132 of 136)	97.1 % (132 of 136)
Backbone	95.3 % (41 of 43)	95.3 % (41 of 43)
Sidechain	97.8 % (91 of 93)	97.8 % (91 of 93)
Aromatic	100.0 % (15 of 15)	100.0 % (15 of 15)
Methyl	100.0 % (6 of 6)	100.0 % (6 of 6)

1. Human peptide hormone

HSDGIFTDSY SRYRKQMAVK KX

Show sample condition

Sample

Pressure 1 atm, Temperature 298 (±0.1) K, pH 6.3 (±0.05)

#	Name	Isotope labeling	Type	Concentration
1	Human peptide hormone			1.7 mM
2	G-protein coupled receptor (GPCR)			0.0 ~ 0.0 mM

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Protein Blocks Logo

Calculated from 25 models in PDB: 1GEA, Strand ID: A Detail

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Release date

2008-07-15

Citation 1

Conformation of a peptide ligand bound to its G-protein coupled receptor
Ogi, K., Inooka, H., Ohtaki, T., Kitahara, O., Ikegami, T., Endo, S., Kitada, C., Onda, H., Fujino, M., Shirakawa, M.
Nat. Struct. Biol. (2001), 8, 161-165, PubMed 11175907 , DOI 10.1038/84159 , Abstract

Show more 1 citaion

Citation 2

X-PLOR, version 3.1: A system for X-ray crystallography and NMR
Brunger, A.T.

Hide non-primary 1 citaion

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Human peptide hormone, : 1 : 16 : 45 entities Detail

Interaction partners 1. Human peptide hormone, : 4 interactors Detail

Experiments performed 1 experiments Detail