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1H and 15N Chemical Shift Assignments for a Cys58Ser mutant of TFF1
Authors
Williams, M.A., Polshakov, V.I., Feeney, J.
Assembly
Human Trefoil Factor Family 1 protein
Entity
1. Human Trefoil Factor Family 1 protein (polymer, Thiol state: all disulfide bound), 60 monomers, 6658.265 Da Detail

EAQTETCTVA PRERQNCGFP GVTPSQCANK GCCFDDTVRG VPWCFYPNTI DVPPEEESEF


Formula weight
6658.265 Da
Entity Connection
disulfide 3 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing0:CYS7:SG1:CYS33:SG
2disulfidesing0:CYS17:SG1:CYS32:SG
3disulfidesing0:CYS27:SG1:CYS44:SG

Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
SIMULATED ANNEALING
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 91.6 %, Completeness (bb): 91.2 % Detail

Polymer type: polypeptide(L)

Total1H15N
All91.6 % (371 of 405)90.4 % (312 of 345)98.3 % (59 of 60)
Backbone91.2 % (155 of 170)88.0 % (103 of 117)98.1 % (52 of 53)
Sidechain91.9 % (216 of 235)91.7 % (209 of 228)100.0 % (7 of 7)
Aromatic90.3 % (28 of 31)90.0 % (27 of 30)100.0 % (1 of 1)
Methyl95.2 % (20 of 21)95.2 % (20 of 21)

1. Trefoil Factor Family 1

EAQTETCTVA PRERQNCGFP GVTPSQCANK GCCFDDTVRG VPWCFYPNTI DVPPEEESEF

Sample #1

Temperature 298 (±1) K, pH 5.8 (±0.2)


#NameIsotope labelingTypeConcentration
1Trefoil Factor Family 11.8 mM
2sodium phosphate5 mM
Sample #2

Temperature 298 (±1) K, pH 5.8 (±0.2)


#NameIsotope labelingTypeConcentration
3Trefoil Factor Family 1[U-95% 15N]1.3 mM
4sodium phosphate5 mM

Protein Blocks Logo
Calculated from 19 models in PDB: 1PS2, Strand ID: A Detail


Release date
2001-02-14
Citation 1
NMR-based structural studies of the pNR-2/pS2 single domain trefoil peptide. Similarities to porcine spasmolytic peptide and evidence for a monomeric structure
Polshakov, V.I., Thomas, F.A., Westley, B.R., Chadwick, M.P., May, F.E.B., Carr, M.D., Feeney, J.
Eur. J. Biochem. (1995), 233, 847-855, PubMed 8521850 , DOI: ,
Citation 2
High-resolution solution structure of human pNR-2/pS2: a single trefoil motif protein
J. Mol. Biol. (1997), 267, 418-432, PubMed 9096235 , DOI 10.1006/jmbi.1997.0896 ,
Related entities 1. Human Trefoil Factor Family 1 protein, : 1 : 3 : 39 entities Detail
Interaction partners 1. Human Trefoil Factor Family 1 protein, : 16 interactors Detail
Experiments performed 8 experiments Detail
nullKeywords trefoil, ps2, pNR-2, TFF1