1H and 15N Chemical Shift Assignments for a Cys58Ser mutant of TFF1
ID | Type | Value order | Atom ID 1 | Atom ID 2 |
---|---|---|---|---|
1 | disulfide | sing | 0:CYS7:SG | 1:CYS33:SG |
2 | disulfide | sing | 0:CYS17:SG | 1:CYS32:SG |
3 | disulfide | sing | 0:CYS27:SG | 1:CYS44:SG |
Polymer type: polypeptide(L)
Total | 1H | 15N | |
---|---|---|---|
All | 91.6 % (371 of 405) | 90.4 % (312 of 345) | 98.3 % (59 of 60) |
Backbone | 91.2 % (155 of 170) | 88.0 % (103 of 117) | 98.1 % (52 of 53) |
Sidechain | 91.9 % (216 of 235) | 91.7 % (209 of 228) | 100.0 % (7 of 7) |
Aromatic | 90.3 % (28 of 31) | 90.0 % (27 of 30) | 100.0 % (1 of 1) |
Methyl | 95.2 % (20 of 21) | 95.2 % (20 of 21) |
1. Trefoil Factor Family 1
EAQTETCTVA PRERQNCGFP GVTPSQCANK GCCFDDTVRG VPWCFYPNTI DVPPEEESEFTemperature 298 (±1) K, pH 5.8 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | Trefoil Factor Family 1 | 1.8 mM | ||
2 | sodium phosphate | 5 mM |
Temperature 298 (±1) K, pH 5.8 (±0.2)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
3 | Trefoil Factor Family 1 | [U-95% 15N] | 1.3 mM | |
4 | sodium phosphate | 5 mM |