BMRBj - BMREntryMaster

Found 1 Document (1.771 seconds)

BMRB: 4941

1JJR

The Three-dimensional Structure of the C-terminal DNA-binding Domain of Human Ku70

Authors

Zhang, Z., Zhu, L., Lin, D., Chen, F., Chen, D.J., Chen, Y.

Assembly

Ku70 DNA binding domain at the C-terminus

Entity

1. Ku70 DNA binding domain at the C-terminus (polymer, Thiol state: all free), 97 monomers, 10795.12 Da Detail

MGSSHHHHHH SSGLVPRGSH MASPEGKVTK RKHDNEGSGS KRPKVEYSEE ELKTHISKGT LGKFTVPMLK EACRAYGLKS GLKKQELLEA LTKHFQD

Formula weight

10795.12 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 91.8 %, Completeness: 79.9 %, Completeness (bb): 74.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.9 % (897 of 1123)	88.2 % (523 of 593)	66.8 % (290 of 434)	87.5 % (84 of 96)
Backbone	74.2 % (426 of 574)	88.5 % (177 of 200)	59.8 % (168 of 281)	87.1 % (81 of 93)
Sidechain	86.2 % (548 of 636)	88.0 % (346 of 393)	82.9 % (199 of 240)	100.0 % (3 of 3)
Aromatic	42.1 % (32 of 76)	52.6 % (20 of 38)	31.6 % (12 of 38)
Methyl	97.3 % (72 of 74)	97.3 % (36 of 37)	97.3 % (36 of 37)

1. Ku70

MGSSHHHHHH SSGLVPRGSH MASPEGKVTK RKHDNEGSGS KRPKVEYSEE ELKTHISKGT LGKFTVPMLK EACRAYGLKS GLKKQELLEA LTKHFQD

Show sample condition

Sample #1

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	Ku70	[U-15N]	0.8 ~ 1.5 mM
2	phosphate buffer		100 mM
3	NaN3		0.03 %
4	DTT		5 mM

Sample #2

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	Ku70	[U-13C; U-15N]	0.8 ~ 1.5 mM
6	phosphate buffer		100 mM
7	NaN3		0.03 %
8	DTT		5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
²H	DSS	methyl protons	null	indirect	0.1535061
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.3 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
²H	DSS	methyl protons	null	indirect	0.1535061
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.3 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
²H	DSS	methyl protons	null	indirect	0.1535061
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.11 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1JJR, Strand ID: A Detail

Release date

2002-01-22

Citation

The three-dimensional structure of the C-terminal DNA-binding domain of human Ku70
Zhang, Z., Zhu, L., Lin, D., Chen, F., Chen, D.J., Chen, Y.
J. Biol. Chem. (2001), 276, 38231-38236, PubMed 11457852 , DOI 10.1074/jbc.M105238200 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P12956 released on 1990-01-01
Title XRCC6_HUMAN Entity X-ray repair cross-complementing protein 6

Related entities 1. Ku70 DNA binding domain at the C-terminus, : 1 : 9 entities Detail

Experiments performed 11 experiments Detail

1 HNCACB

Instrument

Model Varian Unity-plus (500 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	Ku70	[U-15N]	0.8 ~ 1.5 mM
2	phosphate buffer		100 mM
3	NaN3		0.03 %
4	DTT		5 mM

Sample #2

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	Ku70	[U-13C; U-15N]	0.8 ~ 1.5 mM
6	phosphate buffer		100 mM
7	NaN3		0.03 %
8	DTT		5 mM

2 C(CO)NH

Instrument

Model Varian Unity-plus (500 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	Ku70	[U-15N]	0.8 ~ 1.5 mM
2	phosphate buffer		100 mM
3	NaN3		0.03 %
4	DTT		5 mM

Sample #2

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	Ku70	[U-13C; U-15N]	0.8 ~ 1.5 mM
6	phosphate buffer		100 mM
7	NaN3		0.03 %
8	DTT		5 mM

3 CBCA(CO)NH

Instrument

Model Varian Unity-plus (500 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	Ku70	[U-15N]	0.8 ~ 1.5 mM
2	phosphate buffer		100 mM
3	NaN3		0.03 %
4	DTT		5 mM

Sample #2

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	Ku70	[U-13C; U-15N]	0.8 ~ 1.5 mM
6	phosphate buffer		100 mM
7	NaN3		0.03 %
8	DTT		5 mM

4 HCCH_TOCSY

Instrument

Model Varian Unity-plus (500 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	Ku70	[U-15N]	0.8 ~ 1.5 mM
2	phosphate buffer		100 mM
3	NaN3		0.03 %
4	DTT		5 mM

Sample #2

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	Ku70	[U-13C; U-15N]	0.8 ~ 1.5 mM
6	phosphate buffer		100 mM
7	NaN3		0.03 %
8	DTT		5 mM

5 15N-edited

Instrument

Model Varian Unity-plus (500 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	Ku70	[U-15N]	0.8 ~ 1.5 mM
2	phosphate buffer		100 mM
3	NaN3		0.03 %
4	DTT		5 mM

Sample #2

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	Ku70	[U-13C; U-15N]	0.8 ~ 1.5 mM
6	phosphate buffer		100 mM
7	NaN3		0.03 %
8	DTT		5 mM

6 TOCSY-HSQC

Instrument

Model Varian Unity-plus (500 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	Ku70	[U-15N]	0.8 ~ 1.5 mM
2	phosphate buffer		100 mM
3	NaN3		0.03 %
4	DTT		5 mM

Sample #2

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	Ku70	[U-13C; U-15N]	0.8 ~ 1.5 mM
6	phosphate buffer		100 mM
7	NaN3		0.03 %
8	DTT		5 mM

7 DQFCOSY

Instrument

Model Varian Unity-plus (500 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	Ku70	[U-15N]	0.8 ~ 1.5 mM
2	phosphate buffer		100 mM
3	NaN3		0.03 %
4	DTT		5 mM

Sample #2

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	Ku70	[U-13C; U-15N]	0.8 ~ 1.5 mM
6	phosphate buffer		100 mM
7	NaN3		0.03 %
8	DTT		5 mM

8 13C-edited

Instrument

Model Varian Unity-plus (500 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	Ku70	[U-15N]	0.8 ~ 1.5 mM
2	phosphate buffer		100 mM
3	NaN3		0.03 %
4	DTT		5 mM

Sample #2

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	Ku70	[U-13C; U-15N]	0.8 ~ 1.5 mM
6	phosphate buffer		100 mM
7	NaN3		0.03 %
8	DTT		5 mM

9 NOESY

Instrument

Model Varian Unity-plus (500 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	Ku70	[U-15N]	0.8 ~ 1.5 mM
2	phosphate buffer		100 mM
3	NaN3		0.03 %
4	DTT		5 mM

Sample #2

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	Ku70	[U-13C; U-15N]	0.8 ~ 1.5 mM
6	phosphate buffer		100 mM
7	NaN3		0.03 %
8	DTT		5 mM

10 H(CCO)NH

Instrument

Model Varian Unity-plus (500 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	Ku70	[U-15N]	0.8 ~ 1.5 mM
2	phosphate buffer		100 mM
3	NaN3		0.03 %
4	DTT		5 mM

Sample #2

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	Ku70	[U-13C; U-15N]	0.8 ~ 1.5 mM
6	phosphate buffer		100 mM
7	NaN3		0.03 %
8	DTT		5 mM

11 HBHA(CO)NH

Instrument

Model Varian Unity-plus (500 MHz)

Sample #1

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
1	Ku70	[U-15N]	0.8 ~ 1.5 mM
2	phosphate buffer		100 mM
3	NaN3		0.03 %
4	DTT		5 mM

Sample #2

Temperature 293 (±0.1) K, pH 6.0 (±0.05)

#	Name	Isotope labeling	Concentration
5	Ku70	[U-13C; U-15N]	0.8 ~ 1.5 mM
6	phosphate buffer		100 mM
7	NaN3		0.03 %
8	DTT		5 mM

nullKeywords DNA binding domain, Ku70, NMR assignment

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Found 1 Document (1.771 seconds)

BMRB: 4941

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. Ku70 DNA binding domain at the C-terminus, : 1 : 9 entities Detail

Experiments performed 11 experiments Detail

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