BMRB: 4946

Solution Structures of C-1027 Apoprotein and its Complex with the Aromatized Chromophore

Authors

Tanaka, T., Fukuda-Ishisaka, S., Hirama, M., Otani, T.

Assembly

C-1027 apoprotein

Entity

1. C-1027 apoprotein (polymer, Thiol state: all disulfide bound), 110 monomers, 10501.31 Da Detail

APAFSVSPAS GLSDGQSVSV SVSGAAAGET YYIAQCAPVG GQDACNPATA TSFTTDASGA ASFSFVVRKS YTGSTPEGTP VGSVDCATAA CNLGAGNSGL DLGHVALTFG

Formula weight

10501.31 Da

Entity Connection

disulfide 2 Detail

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ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS36:SG	1:CYS45:SG
2	disulfide	sing	1:CYS86:SG	1:CYS91:SG

Source organism

Streptomyces globisporus

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.4 %, Completeness (bb): 99.6 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	99.4 % (521 of 524)	99.4 % (521 of 524)
Backbone	99.6 % (228 of 229)	99.6 % (228 of 229)
Sidechain	99.3 % (293 of 295)	99.3 % (293 of 295)
Aromatic	100.0 % (39 of 39)	100.0 % (39 of 39)
Methyl	100.0 % (61 of 61)	100.0 % (61 of 61)

1. C-1027 apoprotein

APAFSVSPAS GLSDGQSVSV SVSGAAAGET YYIAQCAPVG GQDACNPATA TSFTTDASGA ASFSFVVRKS YTGSTPEGTP VGSVDCATAA CNLGAGNSGL DLGHVALTFG

Show sample condition

Sample

Temperature 303 (±1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	C-1027 apoprotein			6.6 ~ 7.6 mM

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Protein Blocks Logo

Calculated from 30 models in PDB: 1HZK, Strand ID: A Detail

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Release date

2001-08-21

Citation

Solution structures of C-1027 apoprotein and its complex with the aromatized chromophore
Tanaka, T., Fukuda-Ishisaka, S., Hirama, M., Otani, T.
J. Mol. Biol. (2001), 309, 267-283, PubMed 11491295 , DOI 10.1006/jmbi.2001.4621 , Abstract

Related entities 1. C-1027 apoprotein, : 1 : 3 : 1 : 18 entities Detail

Experiments performed 3 experiments Detail