BMRBj - BMREntryMaster

Found 1 Document (0.431 seconds)

BMRB: 495

1JOK

Two-Dimensional NMR Studies of Staphylococcal Nuclease. 1. Sequence-Specific Assignments of Hydrogen-1 Signals and Solution Structure of the Nuclease H124L-Thymidine 3',5'-Bisphosphate-Ca2+ Ternary Complex

Authors

Wang, J., LeMaster, D.M., Markley, J.L.

Assembly

micrococcal nuclease

Entity

1. micrococcal nuclease (polymer), 143 monomers, 16214.55 Da Detail

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SED

Formula weight

16214.55 Da

Source organism

Staphylococcus aureus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.8 %, Completeness: 51.1 %, Completeness (bb): 50.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	51.1 % (466 of 912)	51.1 % (466 of 912)
Backbone	50.2 % (145 of 289)	50.2 % (145 of 289)
Sidechain	51.5 % (321 of 623)	51.5 % (321 of 623)
Aromatic	90.9 % (50 of 55)	90.9 % (50 of 55)
Methyl	69.3 % (52 of 75)	69.3 % (52 of 75)

1. micrococcal nuclease

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SED

Show sample condition

Sample

Temperature 318 K, pH 5.5

Release date

1995-07-30

Citation

Two-dimensional NMR studies of staphylococcal nuclease. 1. Sequence-specific assignments of hydrogen-1 signals and solution structure of the nuclease H124L-thymidine 3',5'-bisphosphate-Ca2+ ternary complex
Wang, J., LeMaster, D.M., Markley, J.L.
Biochemistry (1990), 29, 88-101, PubMed 2108720 , DOI: , Abstract

Related entities 1. micrococcal nuclease, : 1 : 8 : 43 : 87 entities Detail

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr495_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr495_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_assignment_data_set_one	Warning	538		88.1 (chain: 1, length: 143)

Assigned chemical shifts

Saveframe: chemical_shift_assignment_data_set_one
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 143, Sequence coverage: 88.1%
- Total number of assignments
  - ¹H chemical shifts: 538
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

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Found 1 Document (0.431 seconds)

BMRB: 495

Sample

Related entities 1. micrococcal nuclease, : 1 : 8 : 43 : 87 entities Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail