BMRBj - BMREntryMaster

Found 1 Document (0.504 seconds)

BMRB: 496

1JOK

Two-Dimensional NMR Studies of Staphylococcal Nuclease. 2. Sequence-Specific Assignments of Carbon-13 and Nitrogen-15 Signals from the Nuclease H124L-Thymidine 3',5'-Bisphosphate-Ca2+ Ternary Complex

Authors

Wang, J., Hinck, A.P., Loh, S.N., Markley, J.L.

Assembly

micrococcal nuclease

Entity

1. micrococcal nuclease (polymer), 143 monomers, 16214.55 Da Detail

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SED

Formula weight

16214.55 Da

Source organism

Staphylococcus aureus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 73.4 %, Completeness: 70.9 %, Completeness (bb): 76.6 % Detail

Polymer type: polypeptide(L)

	Total	¹⁵N
All	70.9 % (105 of 148)	70.9 % (105 of 148)
Backbone	76.6 % (105 of 137)	76.6 % (105 of 137)
Sidechain	0.0 % (0 of 11)	0.0 % (0 of 11)
Aromatic	0.0 % (0 of 1)	0.0 % (0 of 1)

1. micrococcal nuclease

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SED

Show sample condition

Sample

Temperature 318 K, pH 5.1

Release date

1995-07-30

Citation

Two-dimensional NMR studies of staphylococcal nuclease. 2. Sequence-specific assignments of carbon-13 and nitrogen-15 signals from the nuclease H124L-thymidine 3',5'-bisphosphate-Ca2+ ternary complex
Wang, J., Hinck, A.P., Loh, S.N., Markley, J.L.
Biochemistry (1990), 29, 102-113, PubMed 2322533 , DOI: , Abstract

Related entities 1. micrococcal nuclease, : 1 : 8 : 43 : 87 entities Detail

Experiments performed 1 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr496_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr496_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	chemical_shift_assignment_data_set_one	Warning	104		72.7 (chain: 1, length: 143)

Assigned chemical shifts

Saveframe: chemical_shift_assignment_data_set_one
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 143, Sequence coverage: 72.7%
- Total number of assignments
  - ¹⁵N chemical shifts: 104
- Completeness of assignments
  - Entity_assemble_ID: 1
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

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Found 1 Document (0.504 seconds)

BMRB: 496

Sample

Related entities 1. micrococcal nuclease, : 1 : 8 : 43 : 87 entities Detail

Experiments performed 1 experiments Detail

Instrument

Sample

Chemical shift validation 3 contents Detail