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BMRB: 4961

1H, 15N and 13C assignments of the N-terminal domain of Yersinia outer protein H in its apo form and in complex with a phosphotyrosine peptide representing the putative binding site on host protein target p130Cas

Authors

Khandelwal, P., Keliikuli, K., Smith, C.L., Saper, M.A., Zuiderweg, E.R.P.

Assembly

YopH complex

Entity

1. Yersinia outer protein H (polymer, Thiol state: all free), 136 monomers, 14859.41 Da Detail

MNLSLSDLHR QVSRLVQQES GDCTGKLRGN VAANKETTFQ GLTIASGARE SEKVFAQTVL SHVANVVLTQ EDTAKLLQST VKHNLNNYDL RSVGNGNSVL VSLRSDQMTL QDAKVLLEAA LRQESGARGS HHHHHH

2. DEpYDDPF (polymer, Thiol state: not present), 7 monomers, 979.8333 Da Detail

DEXDDPF

Total weight

15839.243 Da

Max. entity weight

14859.41 Da

Source organism

Yersinia pestis

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 88.8 %, Completeness: 70.8 %, Completeness (bb): 85.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	70.8 % (1118 of 1578)	62.5 % (509 of 814)	80.5 % (486 of 604)	76.9 % (123 of 160)
Backbone	85.6 % (728 of 850)	84.2 % (246 of 292)	86.3 % (360 of 417)	86.5 % (122 of 141)
Sidechain	58.1 % (500 of 861)	50.0 % (261 of 522)	74.7 % (239 of 320)	0.0 % (0 of 19)
Aromatic	10.8 % (8 of 74)	10.8 % (4 of 37)	10.8 % (4 of 37)
Methyl	86.3 % (145 of 168)	92.9 % (78 of 84)	79.8 % (67 of 84)

1. Yersinia outer protein H

MNLSLSDLHR QVSRLVQQES GDCTGKLRGN VAANKETTFQ GLTIASGARE SEKVFAQTVL SHVANVVLTQ EDTAKLLQST VKHNLNNYDL RSVGNGNSVL VSLRSDQMTL QDAKVLLEAA LRQESGARGS HHHHHH

2. DEpYDDPF

DEXDDPF

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Yersinia outer protein H	[U-13C; U-15N]		0.6 ~ 0.9 mM
2	DEpYDDPF			0.6 ~ 0.9 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.43 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.43 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.11 ppm, Outliers: 2 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.55 ppm, Outliers: 1 Detail

Release date

2001-03-31

Citation

Letter to the Editor: 1H, 15N and 13C assignments of the N-terminal domain of Yersinia outer protein H in its apo form and in complex with a phosphotyrosine peptide
Khandelwal, P., Keliikuli, K., Smith, C.L., Saper, M.A., Zuiderweg, E.R.P.
J. Biomol. NMR (2001), 21, 69-70, PubMed , DOI:

Related entities 1. Yersinia outer protein H, : 1 : 2 entities Detail

Experiments performed 10 experiments Detail

1 HNCA