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Two-Dimensional NMR Studies of Staphylococcal Nuclease. 2. Sequence-Specific Assignments of Carbon-13 and Nitrogen-15 Signals from the Nuclease H124L-Thymidine 3',5'-Bisphosphate-Ca2+ Ternary Complex
Authors
Wang, J., Hinck, A.P., Loh, S.N., Markley, J.L.
Assembly
micrococcal nuclease
Entity
1. micrococcal nuclease (polymer), 143 monomers, 16214.55 Da Detail

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SED


Formula weight
16214.55 Da
Source organism
Staphylococcus aureus
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 65.7 %, Completeness: 25.9 %, Completeness (bb): 32.4 % Detail

Polymer type: polypeptide(L)

Total13C
All25.9 % (173 of 669)25.9 % (173 of 669)
Backbone32.4 % (136 of 420)32.4 % (136 of 420)
Sidechain20.6 % (79 of 383)20.6 % (79 of 383)
Aromatic 0.0 % (0 of 54) 0.0 % (0 of 54)
Methyl49.3 % (37 of 75)49.3 % (37 of 75)

1. micrococcal nuclease

ATSTKKLHKE PATLIKAIDG DTVKLMYKGQ PMTFRLLLVD TPETKHPKKG VEKYGPEASA FTKKMVENAK KIEVEFDKGQ RTDKYGRGLA YIYADGKMVN EALVRQGLAK VAYVYKPNNT HEQLLRKSEA QAKKEKLNIW SED

Sample

Temperature 318 K, pH 5.5



LACS Plot; CA
Referencing offset: 2.03 ppm, Outliers: 3 Detail
LACS Plot; CB
Referencing offset: 2.03 ppm, Outliers: 3 Detail
Release date
1995-07-30
Citation
Two-dimensional NMR studies of staphylococcal nuclease. 2. Sequence-specific assignments of carbon-13 and nitrogen-15 signals from the nuclease H124L-thymidine 3',5'-bisphosphate-Ca2+ ternary complex
Wang, J., Hinck, A.P., Loh, S.N., Markley, J.L.
Biochemistry (1990), 29, 102-113, PubMed 2322533 , DOI 10.1021/bi00453a012 ,
Related entities 1. micrococcal nuclease, : 1 : 7 : 43 : 73 entities Detail
Experiments performed 1 experiments Detail