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BMRB: 4979


4979
1HA8
1H and 13C chemical shift assignments for the pheromone Er-23 from Euplotes raikovi
Authors
Zahn, R., Damberger, F.F., Ortenzi, C., Luporini, P., Wuthrich, K.
Assembly
Er-23 pheromone
Entity
1. Er-23 pheromone (polymer, Thiol state: all disulfide bound), 51 monomers, 5104.517 Da Detail

GECEQCFSDG GDCTTCFNNG TGPCANCLAG YPAGCSNSDC TAFLSQCYGG C


Formula weight
5104.517 Da
Entity Connection
disulfide 5 Detail
IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing0:CYS3:SG1:CYS24:SG
2disulfidesing0:CYS6:SG1:CYS16:SG
3disulfidesing0:CYS13:SG1:CYS47:SG
4disulfidesing0:CYS27:SG1:CYS40:SG
5disulfidesing0:CYS35:SG1:CYS51:SG

Source organism
Euplotes raikovi
Exptl. method
NMR
Refine. method
TORSION ANGLE DYNAMICS
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 70.3 %, Completeness (bb): 66.0 % Detail
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Polymer type: polypeptide(L)

Total1H13C
All70.3 % (303 of 431)98.8 % (243 of 246)32.4 % (60 of 185)
Backbone66.0 % (167 of 253)99.1 % (108 of 109)41.0 % (59 of 144)
Sidechain63.6 % (140 of 220)98.5 % (135 of 137) 6.0 % (5 of 83)
Aromatic45.7 % (21 of 46)91.3 % (21 of 23) 0.0 % (0 of 23)
Methyl50.0 % (12 of 24)100.0 % (12 of 12) 0.0 % (0 of 12)

1. Er-23 pheromone

GECEQCFSDG GDCTTCFNNG TGPCANCLAG YPAGCSNSDC TAFLSQCYGG C

Show sample condition
Sample

Temperature 290 (±1) K, pH 4.6 (±0.1)


#NameIsotope labelingTypeConcentration
1Er-23 pheromone1.0 mM
2H2O90 %
3D2O10 %

Protein Blocks Logo
Calculated from 20 models in PDB: 1HA8, Strand ID: A Detail

Release date
2001-11-08
Citation 1
NMR structure of the Euplotes raikovi pheromone Er-23 and identification of its five disulfide bonds
Zahn, R., Damberger, F.F., Ortenzi, C., Luporini, P., Wuthrich, K.
J. Mol. Biol. (2001), 313, 923-931, PubMed 11700049 , DOI 10.1006/jmbi.2001.5099 ,
Show more 4 citaions
Citation 2
NMR structure of the pheromone Er-22 from Euplotes raikovi
Liu, A., Luginbuhl, P., Zerbe, O., Ortenzi, C., Luporini, P., Wuthrich, K.
J. Biomol. NMR (2001), 19, 75-78, PubMed 11246857 , DOI 10.2210/pdb1ha8/pdb ,
Citation 3
The program XEASY for computer-supported NMR spectral analysis of biological macromolecules
J. Biomol. NMR (1995), 6, 1-10, PubMed 22911575 , DOI 10.1007/BF00417486 ,
Citation 4
Torsion angle dynamics for NMR structure calculation with the new program DYANA
Guntert, P., Mumenthaler, C., Wuthrich, K.
J. Mol. Biol. (1997), 273, 283-298, PubMed 9367762 , DOI 10.1006/jmbi.1997.1284 ,
Citation 5
The new program OPAL for molecular dynamics simulations and energy refinements of biological macromolecules
Luginbuhl, P., Guntert, P., Billeter, M., Wuthrich, K.
J. Biomol. NMR (1996), 8, 136-146, PubMed 8914272 , DOI 10.1007/bf00211160 ,
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Related entities 1. Er-23 pheromone, : 1 : 1 : 6 entities Detail
More details for 8 related entities
Experiments performed 4 experiments Detail
nullKeywords cell-signaling, ciliate pheromone, disulfide bond identification, disulfide-rich, Er-23, Euplotes raikovi, GA group