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Backbone 1H, 15N, 13C and side chain 13C assignment of YUH1-Ub in a 35 kDa complex
Authors
Rajesh, S.
Assembly
YUH1
Entity
1. Yuh1 (polymer, Thiol state: all other bound), 236 monomers, 26385.03 Da Detail

MSGENRAVVP IESNPEVFTN FAHKLGLKNE WAYFDIYSLT EPELLAFLPR PVKAIVLLFP INEDRKSSTS QQITSSYDVI WFKQSVKNAC GLYAILHSLS NNQSLLEPGS DLDNFLKSQS DTSSSKNRFD DVTTDQFVLN VIKENVQTFS TGQSEAPEAT ADTNLHYITY VEENGGIFEL DGRNLSGPLY LGKSDPTATD LIEQELVRVR VASYMENANE EDVLNFAMLG LGPNWE


2. Ubiquitin (polymer, Thiol state: not present), 76 monomers, 8550.705 Da Detail

MQIFVKTLTG KTITLEVEPS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGX


Total weight
34935.734 Da
Max. entity weight
26385.03 Da
Entity Connection
thioester 1 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1thioestersing1:CYS90:SG2:GLZ76:O

Source organism
Saccharomyces cerevisiae
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete1
Sequence coverage: 77.9 %, Completeness: 43.3 %, Completeness (bb): 65.2 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All43.3 % (1579 of 3647)23.3 % (439 of 1886)64.4 % (910 of 1414)66.3 % (230 of 347)
Backbone65.2 % (1187 of 1821)43.9 % (267 of 608)75.8 % (694 of 916)76.1 % (226 of 297)
Sidechain29.2 % (620 of 2120)13.5 % (173 of 1278)55.9 % (443 of 792) 8.0 % (4 of 50)
Aromatic 0.8 % (2 of 264) 1.5 % (2 of 132) 0.0 % (0 of 129) 0.0 % (0 of 3)
Methyl38.5 % (140 of 364)16.5 % (30 of 182)60.4 % (110 of 182)

1. Yuh1

MSGENRAVVP IESNPEVFTN FAHKLGLKNE WAYFDIYSLT EPELLAFLPR PVKAIVLLFP INEDRKSSTS QQITSSYDVI WFKQSVKNAC GLYAILHSLS NNQSLLEPGS DLDNFLKSQS DTSSSKNRFD DVTTDQFVLN VIKENVQTFS TGQSEAPEAT ADTNLHYITY VEENGGIFEL DGRNLSGPLY LGKSDPTATD LIEQELVRVR VASYMENANE EDVLNFAMLG LGPNWE

2. Ubiquitin

MQIFVKTLTG KTITLEVEPS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGX

Sample

Temperature 303 (±1) K, pH 6.0 (±0.2)


#NameIsotope labelingTypeConcentration
1Yuh1[U-95% 2H; U-13C; U-15N]2 mM
2Ubiquitin[U-95% 2H; U-13C; U-15N]2 mM

Chem. Shift Complete2
Sequence coverage: 35.6 %, Completeness: 32.2 %, Completeness (bb): 47.2 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All32.2 % (2346 of 7294)18.1 % (683 of 3772)47.0 % (1329 of 2828)48.1 % (334 of 694)
Backbone47.2 % (1720 of 3642)32.5 % (395 of 1216)54.3 % (995 of 1832)55.6 % (330 of 594)
Sidechain22.6 % (957 of 4240)11.3 % (290 of 2556)41.9 % (663 of 1584) 4.0 % (4 of 100)
Aromatic 0.4 % (2 of 528) 0.8 % (2 of 264) 0.0 % (0 of 258) 0.0 % (0 of 6)
Methyl30.8 % (224 of 728)14.8 % (54 of 364)46.7 % (170 of 364)

1. Yuh1

MSGENRAVVP IESNPEVFTN FAHKLGLKNE WAYFDIYSLT EPELLAFLPR PVKAIVLLFP INEDRKSSTS QQITSSYDVI WFKQSVKNAC GLYAILHSLS NNQSLLEPGS DLDNFLKSQS DTSSSKNRFD DVTTDQFVLN VIKENVQTFS TGQSEAPEAT ADTNLHYITY VEENGGIFEL DGRNLSGPLY LGKSDPTATD LIEQELVRVR VASYMENANE EDVLNFAMLG LGPNWE

2. Ubiquitin

MQIFVKTLTG KTITLEVEPS DTIDNVKSKI QDKEGIPPDQ QRLIFAGKQL EDGRTLSDYN IQKESTLHLV LRLRGX

Sample

Temperature 303 (±1) K, pH 6.0 (±0.2)


#NameIsotope labelingTypeConcentration
1Yuh1[U-95% 2H; U-13C; U-15N]2 mM
2Ubiquitin[U-95% 2H; U-13C; U-15N]2 mM

LACS Plot; CA
Referencing offset: 0.85 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: 0.85 ppm, Outliers: 1 Detail
LACS Plot; CO
Referencing offset: 0.44 ppm, Outliers: 1 Detail
Release date
2008-07-16
Citation
Backbone 1H,13C,15N and side chain 13C assignment of YUH1-Ub in a 35 kDa covalent complex
Rajesh, S.
J. Biomol. NMR
Related entities 1. Yuh1, : 1 : 1 : 31 entities Detail
Experiments performed 9 experiments Detail