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Backbone 1H,13C and 15N chemical shift assignment for the maltotriose-bound state of 2H,13C,15N-labeled maltodextrin-binding protein
Authors
Evenas, J., Tugarinov, V., Skrynnikov, N.R., Goto, N.K., Muhandiram, R., Kay, L.E.
Assembly
maltodextrin-binding protein
Entity
1. maltodextrin-binding protein (polymer, Thiol state: not present), 370 monomers, 40694.71 Da Detail

KTEEGKLVIW INGDKGYNGL AEVGKKFEKD TGIKVTVEHP DKLEEKFPQV AATGDGPDII FWAHDRFGGY AQSGLLAEIT PDKAFQDKLY PFTWDAVRYN GKLIAYPIAV EALSLIYNKD LLPNPPKTWE EIPALDKELK AKGKSALMFN LQEPYFTWPL IAADGGYAFK YENGKYDIKD VGVDNAGAKA GLTFLVDLIK NKHMNADTDY SIAEAAFNKG ETAMTINGPW AWSNIDTSKV NYGVTVLPTF KGQPSKPFVG VLSAGINAAS PNKELAKEFL ENYLLTDEGL EAVNKDKPLG AVALKSYEEE LAKDPRIAAT MENAQKGEIM PNIPQMSAFW YAVRTAVINA ASGRQTVDEA LKDAQTRITK


2. MLR (non-polymer), 504.437 Da
Total weight
41199.15 Da
Max. entity weight
40694.71 Da
Source organism
Escherichia coli
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 98.6 %, Completeness: 45.8 %, Completeness (bb): 81.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All45.8 % (1980 of 4322)23.8 % (532 of 2237)65.4 % (1106 of 1692)87.0 % (342 of 393)
Backbone81.0 % (1741 of 2149)49.8 % (358 of 719)96.4 % (1042 of 1081)97.7 % (341 of 349)
Sidechain22.8 % (573 of 2514)11.4 % (173 of 1518)41.9 % (399 of 952) 2.3 % (1 of 44)
Aromatic 6.6 % (25 of 378) 7.9 % (15 of 189) 5.0 % (9 of 181)12.5 % (1 of 8)
Methyl22.7 % (95 of 418)12.9 % (27 of 209)32.5 % (68 of 209)

1. maltodextrin-binding protein

KTEEGKLVIW INGDKGYNGL AEVGKKFEKD TGIKVTVEHP DKLEEKFPQV AATGDGPDII FWAHDRFGGY AQSGLLAEIT PDKAFQDKLY PFTWDAVRYN GKLIAYPIAV EALSLIYNKD LLPNPPKTWE EIPALDKELK AKGKSALMFN LQEPYFTWPL IAADGGYAFK YENGKYDIKD VGVDNAGAKA GLTFLVDLIK NKHMNADTDY SIAEAAFNKG ETAMTINGPW AWSNIDTSKV NYGVTVLPTF KGQPSKPFVG VLSAGINAAS PNKELAKEFL ENYLLTDEGL EAVNKDKPLG AVALKSYEEE LAKDPRIAAT MENAQKGEIM PNIPQMSAFW YAVRTAVINA ASGRQTVDEA LKDAQTRITK

Sample

Temperature 310 (±0.5) K, pH 7.2 (±0.1)


#NameIsotope labelingTypeConcentration
1maltodextrin-binding protein[U-2H; U-15N; U-13C]1.3 mM
2maltotriose1.3 mM

Release date
2001-09-11
Citation
Ligand-induced structural changes to maltodextrin-binding protein as studied by solution NMR spectroscopy
Evenas, J., Tugarinov, V., Skrynnikov, N.R., Goto, N.K., Muhandiram, R., Kay, L.E.
J. Mol. Biol. (2001), 309, 961-974, PubMed 11399072 , DOI 10.1006/jmbi.2001.4695 ,
Related entities 1. maltodextrin-binding protein, : 1 : 4 : 93 : 12 entities Detail
Interaction partners 1. maltodextrin-binding protein, : 1 interactors Detail
Experiments performed 3 experiments Detail
Keywords chemical shift assignment, dipolar couplings, maltodextrin binding protein, protein domains, solution conformation