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Found 1 Document (2.141 seconds)

BMRB: 5013

8R1F

1H, 15N and 13C Assignments of the Catalytic Domain of E6-associated Protein (E6AP)

Authors

Wu, Y., Domaille, P.J.

Assembly

E6AP catalytic domain

Entity

1. E6AP catalytic domain (polymer, Thiol state: all free), 120 monomers, 13662.53 Da Detail

ELLICGSRNL DFQALEETTE YDGGYTRDSV LIREFWEIVH SFTDEQKRLF LQFTTGTDRA PVGGLGKLKM IIAKNGPDTE RLPTSHTCFN VLLLPEYSSK EKLKERLLKA ITYAKGFGML

Formula weight

13662.53 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.3 %, Completeness: 86.1 %, Completeness (bb): 93.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.1 % (1235 of 1434)	84.0 % (627 of 746)	86.9 % (491 of 565)	95.1 % (117 of 123)
Backbone	93.8 % (668 of 712)	94.3 % (232 of 246)	93.1 % (326 of 350)	94.8 % (110 of 116)
Sidechain	80.8 % (672 of 832)	79.0 % (395 of 500)	83.1 % (270 of 325)	100.0 % (7 of 7)
Aromatic	50.8 % (62 of 122)	52.5 % (32 of 61)	48.3 % (29 of 60)	100.0 % (1 of 1)
Methyl	89.3 % (125 of 140)	90.0 % (63 of 70)	88.6 % (62 of 70)

1. E6-associated protein

ELLICGSRNL DFQALEETTE YDGGYTRDSV LIREFWEIVH SFTDEQKRLF LQFTTGTDRA PVGGLGKLKM IIAKNGPDTE RLPTSHTCFN VLLLPEYSSK EKLKERLLKA ITYAKGFGML

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 7.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	E6-associated protein	[U-15N; U-13C]		0.9 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.05 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.06 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.05 ppm, Outliers: 2 Detail

Release date

2002-01-22

Citation

1H, 15N and 13C assignments of the catalytic domain of E6-associated protein (E6AP)
Wu, Y., Domaille, P.J.
J. Biomol. NMR (2001), 21, 285-286, PubMed 11775746 , Abstract

Related entities 1. E6AP catalytic domain, : 1 : 5 : 3 : 2 : 254 entities Detail

Interaction partners 1. E6AP catalytic domain, : 90 interactors Detail

More details for 90 interactors identified by 18 citations

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5013_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5013_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	1357		97.5 (chain: 1, length: 120)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 120, Sequence coverage: 97.5%
- Total number of assignments
  - ¹⁵N chemical shifts: 118
  - ¹H chemical shifts: 747
  - ¹³C chemical shifts: 492
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Peptide bond of PRO
- Tautomeric state of HIS
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	130	118	90.8
¹H chemical shifts	746	617	82.7
¹³C chemical shifts	565	492	87.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	116	111	95.7
¹H chemical shifts	246	234	95.1
¹³C chemical shifts	240	224	93.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	14	7	50.0
¹H chemical shifts	500	383	76.6
¹³C chemical shifts	325	268	82.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	72	65	90.3
¹³C chemical shifts	72	65	90.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹⁵N chemical shifts	1	1	100.0
¹H chemical shifts	61	30	49.2
¹³C chemical shifts	60	29	48.3

Keywords E6AP, ubiquitin ligase

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Found 1 Document (2.141 seconds)

BMRB: 5013

Sample

Chemical shift reference

Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. E6AP catalytic domain, : 1 : 5 : 3 : 2 : 254 entities Detail

Interaction partners 1. E6AP catalytic domain, : 90 interactors Detail

Experiments performed 6 experiments Detail

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Instrument

Sample

Chemical shift validation 3 contents Detail