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BMRB: 5027

1J8K

NMR Structure of Human Fibronectin EDA

Authors

Niimi, T., Osawa, M., Yamaji, N., Yasunaga, K., Sakashita, H., Mase, T., Tanaka, A., Fujita, S.

Assembly

human fibronectin EDA

Entity

1. human fibronectin EDA (polymer, Thiol state: not present), 94 monomers, 10214.05 Da Detail

NIDRPKGLAF TDVDVDSIKI AWESPQGQVS RYRVTYSSPE DGIHELFPAP DGEEDTAELQ GLRPGSEYTV SVVALHDDME SQPLIGTQST AIPA

Formula weight

10214.05 Da

Source organism

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 96.4 %, Completeness (bb): 98.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	96.4 % (997 of 1034)	96.4 % (515 of 534)	96.3 % (392 of 407)	96.8 % (90 of 93)
Backbone	98.4 % (539 of 548)	98.9 % (185 of 187)	98.5 % (271 of 275)	96.5 % (83 of 86)
Sidechain	94.9 % (544 of 573)	95.1 % (330 of 347)	94.5 % (207 of 219)	100.0 % (7 of 7)
Aromatic	82.8 % (53 of 64)	90.6 % (29 of 32)	74.2 % (23 of 31)	100.0 % (1 of 1)
Methyl	100.0 % (102 of 102)	100.0 % (51 of 51)	100.0 % (51 of 51)

1. human fibronectin EDA

NIDRPKGLAF TDVDVDSIKI AWESPQGQVS RYRVTYSSPE DGIHELFPAP DGEEDTAELQ GLRPGSEYTV SVVALHDDME SQPLIGTQST AIPA

Show sample condition

Sample

Temperature 293 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human fibronectin EDA	[U-15N; U-13C]		2.0 mM
2	sodium phosphate			20 mM
3	Na2SO4			400 mM

LACS Plot; CA

Referencing offset: -0.42 ppm, Outliers: 1 Detail

LACS Plot; CB

Referencing offset: -0.42 ppm, Outliers: 1 Detail

LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

LACS Plot; CO

Referencing offset: 0.8 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1J8K, Strand ID: A Detail

Release date

2002-01-22

Citation

NMR structure of human fibronectin EDA
Niimi, T., Osawa, M., Yamaji, N., Yasunaga, K., Sakashita, H., Mase, T., Tanaka, A., Fujita, S.
J. Biomol. NMR (2001), 21, 281-284, PubMed 11775745 , DOI: , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: P02751 released on 1986-07-21
Title FINC_HUMAN Entity Fibronectin

Related entities 1. human fibronectin EDA, : 1 : 3 : 582 entities Detail

Interaction partners 1. human fibronectin EDA, : 90 interactors Detail

More details for 90 interactors identified by 47 citations

Experiments performed 16 experiments Detail

1 2D 1H-13C CT-HSQC

Instrument

Bruker AMX - 600 MHz

Sample

State isotropic, Temperature 293 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human fibronectin EDA	[U-15N; U-13C]		2.0 mM
2	sodium phosphate			20 mM
3	Na2SO4			400 mM

2 2D 1H-15N HSQC

Instrument

Bruker AMX - 600 MHz

Sample

State isotropic, Temperature 293 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human fibronectin EDA	[U-15N; U-13C]		2.0 mM
2	sodium phosphate			20 mM
3	Na2SO4			400 mM

3 3D HNCA

Instrument

Bruker AMX - 600 MHz

Sample

State isotropic, Temperature 293 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human fibronectin EDA	[U-15N; U-13C]		2.0 mM
2	sodium phosphate			20 mM
3	Na2SO4			400 mM

4 3D HN(CO)CA

Instrument

Bruker AMX - 600 MHz

Sample

State isotropic, Temperature 293 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human fibronectin EDA	[U-15N; U-13C]		2.0 mM
2	sodium phosphate			20 mM
3	Na2SO4			400 mM

5 3D HNCO

Instrument

Bruker AMX - 600 MHz

Sample

State isotropic, Temperature 293 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human fibronectin EDA	[U-15N; U-13C]		2.0 mM
2	sodium phosphate			20 mM
3	Na2SO4			400 mM

6 3D HN(CA)CO

Instrument

Bruker AMX - 600 MHz

Sample

State isotropic, Temperature 293 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human fibronectin EDA	[U-15N; U-13C]		2.0 mM
2	sodium phosphate			20 mM
3	Na2SO4			400 mM

7 3D HCACO

Instrument

Bruker AMX - 600 MHz

Sample

State isotropic, Temperature 293 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human fibronectin EDA	[U-15N; U-13C]		2.0 mM
2	sodium phosphate			20 mM
3	Na2SO4			400 mM

8 3D HNCACB

Instrument

Bruker AMX - 600 MHz

Sample

State isotropic, Temperature 293 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human fibronectin EDA	[U-15N; U-13C]		2.0 mM
2	sodium phosphate			20 mM
3	Na2SO4			400 mM

9 3D CBCA(CO)NH

Instrument

Bruker AMX - 600 MHz

Sample

State isotropic, Temperature 293 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human fibronectin EDA	[U-15N; U-13C]		2.0 mM
2	sodium phosphate			20 mM
3	Na2SO4			400 mM

10 3D HBHA(CBCACO)NH

Instrument

Bruker AMX - 600 MHz

Sample

State isotropic, Temperature 293 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human fibronectin EDA	[U-15N; U-13C]		2.0 mM
2	sodium phosphate			20 mM
3	Na2SO4			400 mM

11 3D H(CCO)NH

Instrument

Bruker AMX - 600 MHz

Sample

State isotropic, Temperature 293 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human fibronectin EDA	[U-15N; U-13C]		2.0 mM
2	sodium phosphate			20 mM
3	Na2SO4			400 mM

12 3D C(CO)NH

Instrument

Bruker AMX - 600 MHz

Sample

State isotropic, Temperature 293 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human fibronectin EDA	[U-15N; U-13C]		2.0 mM
2	sodium phosphate			20 mM
3	Na2SO4			400 mM

13 3D HCCH-TOCSY

Instrument

Bruker AMX - 600 MHz

Sample

State isotropic, Temperature 293 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human fibronectin EDA	[U-15N; U-13C]		2.0 mM
2	sodium phosphate			20 mM
3	Na2SO4			400 mM

14 3D 13C-edited NOESY-HSQC

Instrument

Bruker AMX - 600 MHz

Sample

State isotropic, Temperature 293 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human fibronectin EDA	[U-15N; U-13C]		2.0 mM
2	sodium phosphate			20 mM
3	Na2SO4			400 mM

15 3D 15N-edited NOESY-HSQC

Instrument

Bruker AMX - 600 MHz

Sample

State isotropic, Temperature 293 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human fibronectin EDA	[U-15N; U-13C]		2.0 mM
2	sodium phosphate			20 mM
3	Na2SO4			400 mM

16 3D HNHA

Instrument

Bruker AMX - 600 MHz

Sample

State isotropic, Temperature 293 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	human fibronectin EDA	[U-15N; U-13C]		2.0 mM
2	sodium phosphate			20 mM
3	Na2SO4			400 mM

nullKeywords Fibronectin, EDA, NMR, tertiary structure, folding