BMRBj - BMREntryMaster

Found 1 Document (2.707 seconds)

BMRB: 5047

1JBI

NMR structure of the LCCL Domain and its Implications for DFNA9 Deafness Disorder

Authors

Liepinsh, E., Trexler, M., Kaikkonen, A., Weigelt, J., Banyai, L., Patthy, L., Otting, G.

Assembly

LCCL module of human coch-5b2 protein

Entity

1. LCCL module of human coch-5b2 protein (polymer, Thiol state: all disulfide bound), 100 monomers, 10600.90 Da Detail

TAPIAITCFT RGLDIRKEKA DVLCPGGCPL EEFSVYGNIV YASVSSICGA AVHRGVISNS GGPVRVYSLP GRENYSSVDA NGIQSQMLSR WSASFTVTLE

Formula weight

10600.9 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS8:SG	1:CYS24:SG
2	disulfide	sing	1:CYS28:SG	1:CYS48:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

torsion angle dynamics,simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 99.7 %, Completeness (bb): 99.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	99.7 % (656 of 658)	99.8 % (555 of 556)	99.0 % (101 of 102)
Backbone	99.3 % (298 of 300)	99.5 % (204 of 205)	98.9 % (94 of 95)
Sidechain	100.0 % (358 of 358)	100.0 % (351 of 351)	100.0 % (7 of 7)
Aromatic	100.0 % (40 of 40)	100.0 % (39 of 39)	100.0 % (1 of 1)
Methyl	100.0 % (59 of 59)	100.0 % (59 of 59)

1. Human coch-5h2 protein

TAPIAITCFT RGLDIRKEKA DVLCPGGCPL EEFSVYGNIV YASVSSICGA AVHRGVISNS GGPVRVYSLP GRENYSSVDA NGIQSQMLSR WSASFTVTLE

Show sample condition

Sample #1

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human coch-5h2 protein			1.0 mM
2	NaCl			0.1 M

Sample #2

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Human coch-5h2 protein	[U-95% 15N]		3.0 mg/mL
4	NaCl			0.1 M

Sample #3

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	Human coch-5h2 protein	[U-95% 15N]		3.3 mg/mL
6	NaCl			0.1 M

Protein Blocks Logo

Calculated from 20 models in PDB: 1JBI, Strand ID: A Detail

Release date

2001-06-05

Citation

NMR structure of the LCCL domain and implications for DFNA9 deafness disorder
Liepinsh, E., Trexler, M., Kaikkonen, A., Weigelt, J., Banyai, L., Patthy, L., Otting, G.
EMBO J. (2001), 20, 5347-5353, PubMed 11574466 , DOI 10.1093/emboj/20.19.5347 , Abstract

Entries sharing articles Swiss-Prot: 1 entries Detail

Swiss-Prot: O43405 released on 2000-05-30
Title COCH_HUMAN Entity Cochlin

Related entities 1. LCCL module of human coch-5b2 protein, : 1 : 2 : 32 entities Detail

Interaction partners 1. LCCL module of human coch-5b2 protein, : 2 interactors Detail

More details for 2 interactors identified by 1 citations

Experiments performed 7 experiments Detail

1 2D 1H-1H DQF-COSY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Varian UnityPlus (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human coch-5h2 protein			1.0 mM
2	NaCl			0.1 M

Sample #2

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Human coch-5h2 protein	[U-95% 15N]		3.0 mg/mL
4	NaCl			0.1 M

Sample #3

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	Human coch-5h2 protein	[U-95% 15N]		3.3 mg/mL
6	NaCl			0.1 M

2 2D 1H-1H HOHAHA

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Varian UnityPlus (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human coch-5h2 protein			1.0 mM
2	NaCl			0.1 M

Sample #2

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Human coch-5h2 protein	[U-95% 15N]		3.0 mg/mL
4	NaCl			0.1 M

Sample #3

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	Human coch-5h2 protein	[U-95% 15N]		3.3 mg/mL
6	NaCl			0.1 M

3 2D 1H-1H NOESY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Varian UnityPlus (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human coch-5h2 protein			1.0 mM
2	NaCl			0.1 M

Sample #2

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Human coch-5h2 protein	[U-95% 15N]		3.0 mg/mL
4	NaCl			0.1 M

Sample #3

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	Human coch-5h2 protein	[U-95% 15N]		3.3 mg/mL
6	NaCl			0.1 M

4 2D 1H-1H ROESY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Varian UnityPlus (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human coch-5h2 protein			1.0 mM
2	NaCl			0.1 M

Sample #2

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Human coch-5h2 protein	[U-95% 15N]		3.0 mg/mL
4	NaCl			0.1 M

Sample #3

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	Human coch-5h2 protein	[U-95% 15N]		3.3 mg/mL
6	NaCl			0.1 M

5 2D 1H-15N HSQC

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Varian UnityPlus (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human coch-5h2 protein			1.0 mM
2	NaCl			0.1 M

Sample #2

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Human coch-5h2 protein	[U-95% 15N]		3.0 mg/mL
4	NaCl			0.1 M

Sample #3

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	Human coch-5h2 protein	[U-95% 15N]		3.3 mg/mL
6	NaCl			0.1 M

6 3D 1H-1H-15N TOCSY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Varian UnityPlus (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human coch-5h2 protein			1.0 mM
2	NaCl			0.1 M

Sample #2

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Human coch-5h2 protein	[U-95% 15N]		3.0 mg/mL
4	NaCl			0.1 M

Sample #3

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	Human coch-5h2 protein	[U-95% 15N]		3.3 mg/mL
6	NaCl			0.1 M

7 3D 1H-1H-15N NOESY

Instrument

#1 Model Bruker DMX (500 MHz)

#2 Model Bruker DMX (600 MHz)

#3 Model Varian UnityPlus (800 MHz)

Sample #1

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Human coch-5h2 protein			1.0 mM
2	NaCl			0.1 M

Sample #2

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Human coch-5h2 protein	[U-95% 15N]		3.0 mg/mL
4	NaCl			0.1 M

Sample #3

Temperature 301 (±1) K, pH 4.9 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	Human coch-5h2 protein	[U-95% 15N]		3.3 mg/mL
6	NaCl			0.1 M

nullKeywords LCCL domain

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Found 1 Document (2.707 seconds)

BMRB: 5047

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Entries sharing articles Swiss-Prot: 1 entries Detail

Related entities 1. LCCL module of human coch-5b2 protein, : 1 : 2 : 32 entities Detail

Interaction partners 1. LCCL module of human coch-5b2 protein, : 2 interactors Detail

Experiments performed 7 experiments Detail

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