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BMRB: 5049

1N6V

Backbone 1H, 13C, and 15N data for the extracellular domain of human IFNAR2

Authors

Chill, J.H., Nivasch, R., Levy, R., Albeck, S., Schreiber, G., Anglister, J.

Assembly

Extracellular domain of subunit 2 of the human receptor for type I interferons

Entity

1. Extracellular domain of subunit 2 of the human receptor for type I interferons (polymer, Thiol state: all disulfide bound), 212 monomers, 24299.20 Da Detail

SYDSPDYTDE SCTFKISLRN FRSILSWELK NHSIVPTHYT LLYTIMSKPE DLKVVKNCAN TTRSFCDLTD EWRSTHEAYV TVLEGFSGNT TLFSCSHNFW LAIDMSFEPP EFEIVGFTNH INVMVKFPSI VEEELQFDLS LVIEEQSEGI VKKHKPEIKG NMSGNFTYII DKLIPNTNYC VSVYLEHSDE QAVIKSPLKC TLLPPGQESE FS

Formula weight

24299.2 Da

Entity Connection

disulfide 3 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS12:SG	1:CYS95:SG
2	disulfide	sing	1:CYS58:SG	1:CYS66:SG
3	disulfide	sing	1:CYS180:SG	1:CYS200:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.2 %, Completeness: 55.6 %, Completeness (bb): 91.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	55.6 % (1402 of 2521)	47.1 % (613 of 1302)	60.4 % (603 of 999)	84.5 % (186 of 220)
Backbone	91.7 % (1146 of 1250)	91.2 % (383 of 420)	91.9 % (578 of 629)	92.0 % (185 of 201)
Sidechain	30.5 % (450 of 1476)	26.1 % (230 of 882)	38.1 % (219 of 575)	5.3 % (1 of 19)
Aromatic	6.2 % (16 of 258)	7.0 % (9 of 129)	5.6 % (7 of 126)	0.0 % (0 of 3)
Methyl	8.3 % (19 of 228)	6.1 % (7 of 114)	10.5 % (12 of 114)

1. Extracellular domain of subunit 2 of the Human receptor for type I interferons

Show sample condition

Sample

Temperature 308 (±1) K, pH 8.0 (±0.2)

#	Name	Isotope labeling	Concentration
1	Extracellular domain of subunit 2 of the Human receptor for type I interferons	[U-97% 13C; U-97% 15N]	0.25 ~ 0.4 mM
2	NaN3		0.02 %
3	NaCl		0.0 ~ 1.0 mM
4	Tris	[U-2H]	25 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.1 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: -0.03 ppm, Outliers: 5 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	null	indirect	0.2514495
¹H	TSP	methyl protons	external	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291

Referencing offset: 0.72 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 22 models in PDB: 1N6U, Strand ID: A Detail

Release date

2001-06-10

Citation

The human interferon receptor: NMR-based modeling, mapping of the IFN-alpha 2 binding site, and observed ligand-induced tightening
Chill, J.H., Nivasch, R., Levy, R., Albeck, S., Schreiber, G., Anglister, J.
Biochemistry (2002), 41, 3575-3585, PubMed 11888273 , DOI: , Abstract

Related entities 1. Extracellular domain of subunit 2 of the human receptor for type I interferons, : 1 : 4 : 5 : 10 entities Detail

Interaction partners 1. Extracellular domain of subunit 2 of the human receptor for type I interferons, : 14 interactors Detail

More details for 14 interactors identified by 7 citations

Experiments performed 9 experiments Detail

1 HNCO