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BMRB: 5055

1IMU

Solution Structure of HI0257, a Bacterial Binding Protein

Authors

Parsons, L., Eisenstein, E., Orban, J.

Assembly

HYPOTHETICAL PROTEIN HI0257

Entity

1. HYPOTHETICAL PROTEIN HI0257 (polymer, Thiol state: not present), 110 monomers, 12471.96 Da Detail

GSHMTLNITS KQMDITPAIR EHLEERLAKL GKWQTQLISP HFVLNKVPNG FSVEASIGTP LGNLLASATS DDMYKAINEV EEKLERQLNK LQHKSESRRA DERLKDSFEN

Formula weight

12471.96 Da

Source organism

Haemophilus influenzae

Exptl. method

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.2 %, Completeness: 81.8 %, Completeness (bb): 95.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	81.8 % (1062 of 1299)	81.3 % (555 of 683)	79.3 % (394 of 497)	95.0 % (113 of 119)
Backbone	95.4 % (622 of 652)	96.8 % (214 of 221)	93.8 % (305 of 325)	97.2 % (103 of 106)
Sidechain	72.1 % (542 of 752)	74.0 % (342 of 462)	68.6 % (190 of 277)	76.9 % (10 of 13)
Aromatic	31.8 % (21 of 66)	60.6 % (20 of 33)	0.0 % (0 of 32)	100.0 % (1 of 1)
Methyl	92.4 % (109 of 118)	94.9 % (56 of 59)	89.8 % (53 of 59)

1. HI0257

GSHMTLNITS KQMDITPAIR EHLEERLAKL GKWQTQLISP HFVLNKVPNG FSVEASIGTP LGNLLASATS DDMYKAINEV EEKLERQLNK LQHKSESRRA DERLKDSFEN

Show sample condition

Sample

Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HI0257	[U-13C; U-15N]		1.3 mM
2	NaPO4			50 mM
3	NaCl			100 mM
4	dtt			3 mM

LACS Plot; CA

Referencing offset: -0.62 ppm, Outliers: 2 Detail

LACS Plot; CB

Referencing offset: -0.62 ppm, Outliers: 2 Detail

LACS Plot; HA

Referencing offset: -0.08 ppm, Outliers: 2 Detail

LACS Plot; CO

Referencing offset: -0.1 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1IMU, Strand ID: A Detail

Release date

2001-10-15

Citation

Solution structure of HI0257, a bacterial ribosome binding protein
Parsons, L., Eisenstein, E., Orban, J.
Biochemistry (2001), 40, 10979-10986, PubMed 11551193 , DOI 10.1021/bi011077i , Abstract

Related entities 1. HYPOTHETICAL PROTEIN HI0257, : 1 : 1 : 61 entities Detail

Experiments performed 11 experiments Detail

1 3D 13C-separated NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HI0257	[U-13C; U-15N]		1.3 mM
2	NaPO4			50 mM
3	NaCl			100 mM
4	dtt			3 mM

2 3D 15N-separated NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HI0257	[U-13C; U-15N]		1.3 mM
2	NaPO4			50 mM
3	NaCl			100 mM
4	dtt			3 mM

3 HNHA

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HI0257	[U-13C; U-15N]		1.3 mM
2	NaPO4			50 mM
3	NaCl			100 mM
4	dtt			3 mM

4 3D HNCACB

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HI0257	[U-13C; U-15N]		1.3 mM
2	NaPO4			50 mM
3	NaCl			100 mM
4	dtt			3 mM

5 3D CBCACONH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HI0257	[U-13C; U-15N]		1.3 mM
2	NaPO4			50 mM
3	NaCl			100 mM
4	dtt			3 mM

6 3D HNCO

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HI0257	[U-13C; U-15N]		1.3 mM
2	NaPO4			50 mM
3	NaCl			100 mM
4	dtt			3 mM

7 3D HBHACONH

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HI0257	[U-13C; U-15N]		1.3 mM
2	NaPO4			50 mM
3	NaCl			100 mM
4	dtt			3 mM

8 3D 15N TOCSY-HSQC

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HI0257	[U-13C; U-15N]		1.3 mM
2	NaPO4			50 mM
3	NaCl			100 mM
4	dtt			3 mM

9 3D HCCH-TOCSY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HI0257	[U-13C; U-15N]		1.3 mM
2	NaPO4			50 mM
3	NaCl			100 mM
4	dtt			3 mM

10 2D TOCSY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HI0257	[U-13C; U-15N]		1.3 mM
2	NaPO4			50 mM
3	NaCl			100 mM
4	dtt			3 mM

11 2D NOESY

Instrument

Bruker DMX - 600 MHz

Sample

State isotropic, Temperature 298 K, pH 7.0

#	Name	Isotope labeling	Type	Concentration
1	HI0257	[U-13C; U-15N]		1.3 mM
2	NaPO4			50 mM
3	NaCl			100 mM
4	dtt			3 mM

nullKeywords DSRNA binding protein, NMR, proteome, solution structure, ribosome