BMRBj - BMREntryMaster

Found 1 Document (1.555 seconds)

BMRB: 5068

1LSG

Recombinant hen lysozyme containing the extra N-terminal Met as the standard reference for the study of hen lysozyme variants

Authors

Noda, Y., Yokota, A., Horii, D., Tominaga, T., Tanisaka, Y., Tachibana, H., Segawa, S.

Assembly

hen egg white lysozyme

Entity

1. hen egg white lysozyme (polymer, Thiol state: all disulfide bound), 130 monomers, 14444.20 Da Detail

MKVFGRCELA AAMKRHGLDN YRGYSLGNWV CAAKFESNFN TQATNRNTDG STDYGILQIN SRWWCNDGRT PGSRNLCNIP CSALLSSDIT ASVNCAKKIV SDGNGMNAWV AWRNRCKGTD VQAWIRGCRL

Formula weight

14444.2 Da

Entity Connection

disulfide 4 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS128:SG
2	disulfide	sing	1:CYS31:SG	1:CYS116:SG
3	disulfide	sing	1:CYS65:SG	1:CYS81:SG
4	disulfide	sing	1:CYS77:SG	1:CYS95:SG

Source organism

Gallus gallus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 98.5 %, Completeness: 88.4 %, Completeness (bb): 97.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	88.4 % (671 of 759)	88.4 % (671 of 759)
Backbone	97.4 % (263 of 270)	97.4 % (263 of 270)
Sidechain	83.4 % (408 of 489)	83.4 % (408 of 489)
Aromatic	100.0 % (65 of 65)	100.0 % (65 of 65)
Methyl	96.6 % (57 of 59)	96.6 % (57 of 59)

1. hen egg white lysozyme

MKVFGRCELA AAMKRHGLDN YRGYSLGNWV CAAKFESNFN TQATNRNTDG STDYGILQIN SRWWCNDGRT PGSRNLCNIP CSALLSSDIT ASVNCAKKIV SDGNGMNAWV AWRNRCKGTD VQAWIRGCRL

Show sample condition

Sample

Temperature 298 (±1) K, pH 3.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hen egg white lysozyme	[U-15N]		1.0 ~ 1.2 mM

Release date

2002-02-26

Citation

NMR structural study of two-disulfide variant of hen lysozyme: 2SS[6-127, 30-115]--a disulfide intermediate with a partly unfolded structure
Noda, Y., Yokota, A., Horii, D., Tominaga, T., Tanisaka, Y., Tachibana, H., Segawa, S.
Biochemistry (2002), 41, 2130-2139, PubMed 11841203 , Abstract

Entries sharing articles BMRB: 6 entries Detail

  BMRB: 7159 released on 2008-11-11
    Title 1H chemical shift assignments of 2SS[6-127, 30-115] at pH 3.8 and 25 C
  BMRB: 7160 released on 2008-11-11
    Title 1H chemical shift assignments of metLYZ at pH 3.8 and 25 C
  BMRB: 6415 released on 2005-04-03
    Title Three-disulfide variant of hen lysozyme: C30A/C115A
  BMRB: 5803 released on 2004-09-13
    Title Three-disulfide variant of hen lysozyme: C64A/C80A
  BMRB: 5804 released on 2004-09-13
    Title Three-disulfide variant of hen lysozyme: C76A/C94A
  BMRB: 5069 released on 2002-02-26
    Title NMR Structural Study of Two-Disulfide Variant of hen Lysozyme: 2SS[6-127, 30-115]-A Disulfide Intermediate with a Partly Unfolded Structure

Related entities 1. hen egg white lysozyme, : 1 : 1 : 104 : 142 entities Detail

Interaction partners 1. hen egg white lysozyme, : 7 interactors Detail

More details for 7 interactors identified by 6 citations

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:7:CYS:SG	1:128:CYS:SG	unknown	unknown	n/a
1:31:CYS:SG	1:116:CYS:SG	unknown	unknown	n/a
1:65:CYS:SG	1:81:CYS:SG	unknown	unknown	n/a
1:77:CYS:SG	1:95:CYS:SG	unknown	unknown	n/a

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	788		98.5 (chain: 1, length: 130)
1	Chemical shift references	chemical_shift_reference	OK	1		No information

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
34	LYS	HZ1	7.33
34	LYS	HZ2	7.33
34	LYS	HZ3	7.33

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	759	669	88.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	270	265	98.1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	489	404	82.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	62	58	93.5

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	65	65	100.0

Keywords Disulfide Intermediate, hen Lysozyme, Protein Folding, Two-Disulfide Variant

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5068_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	True (see coordinates for details)
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

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Found 1 Document (1.555 seconds)

BMRB: 5068

Sample

Entries sharing articles BMRB: 6 entries Detail

Related entities 1. hen egg white lysozyme, : 1 : 1 : 104 : 142 entities Detail

Interaction partners 1. hen egg white lysozyme, : 7 interactors Detail

Experiments performed 6 experiments Detail

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Chemical shift validation 3 contents Detail