BMRBj - BMREntryMaster

Found 1 Document (1.404 seconds)

BMRB: 5069

1LSG

NMR Structural Study of Two-Disulfide Variant of hen Lysozyme: 2SS[6-127, 30-115]-A Disulfide Intermediate with a Partly Unfolded Structure

Authors

Noda, Y., Yokota, A., Horii, D., Tominaga, T., Tanisaka, Y., Tachibana, H., Segawa, S.

Assembly

hen egg white lysozyme

Entity

1. hen egg white lysozyme (polymer, Thiol state: all disulfide bound), 130 monomers, 14315.94 Da Detail

MKVFGRCELA AAMKRHGLDN YRGYSLGNWV CAAKFESNFN TQATNRNTDG STDYGILQIN SRWWANDGRT PGSRNLANIP ASALLSSDIT ASVNAAKKIV SDGNGMNAWV AWRNRCKGTD VQAWIRGCRL

Formula weight

14315.94 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS7:SG	1:CYS128:SG
2	disulfide	sing	1:CYS31:SG	1:CYS116:SG

Source organism

Gallus gallus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 86.9 %, Completeness: 67.2 %, Completeness (bb): 84.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H
All	67.2 % (507 of 755)	67.2 % (507 of 755)
Backbone	84.4 % (228 of 270)	84.4 % (228 of 270)
Sidechain	57.5 % (279 of 485)	57.5 % (279 of 485)
Aromatic	75.4 % (49 of 65)	75.4 % (49 of 65)
Methyl	85.7 % (54 of 63)	85.7 % (54 of 63)

1. hen egg white lysozyme variant

MKVFGRCELA AAMKRHGLDN YRGYSLGNWV CAAKFESNFN TQATNRNTDG STDYGILQIN SRWWANDGRT PGSRNLANIP ASALLSSDIT ASVNAAKKIV SDGNGMNAWV AWRNRCKGTD VQAWIRGCRL

Show sample condition

Sample

Temperature 298 (±1) K, pH 3.8 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	hen egg white lysozyme variant	[U-15N]		1.0 ~ 1.2 mM

Release date

2002-02-26

Citation

NMR structural study of two-disulfide variant of hen lysozyme: 2SS[6-127, 30-115]--a disulfide intermediate with a partly unfolded structure
Noda, Y., Yokota, A., Horii, D., Tominaga, T., Tanisaka, Y., Tachibana, H., Segawa, S.
Biochemistry (2002), 41, 2130-2139, PubMed 11841203 , DOI 10.1021/bi0113418 , Abstract

Entries sharing articles BMRB: 6 entries Detail

  BMRB: 7159 released on 2008-11-11
    Title 1H chemical shift assignments of 2SS[6-127, 30-115] at pH 3.8 and 25 C
  BMRB: 7160 released on 2008-11-11
    Title 1H chemical shift assignments of metLYZ at pH 3.8 and 25 C
  BMRB: 6415 released on 2005-04-03
    Title Three-disulfide variant of hen lysozyme: C30A/C115A
  BMRB: 5803 released on 2004-09-13
    Title Three-disulfide variant of hen lysozyme: C64A/C80A
  BMRB: 5804 released on 2004-09-13
    Title Three-disulfide variant of hen lysozyme: C76A/C94A
  BMRB: 5068 released on 2002-02-26
    Title Recombinant hen lysozyme containing the extra N-terminal Met as the standard reference for the study of hen lysozyme variants

Related entities 1. hen egg white lysozyme, : 1 : 105 : 141 entities Detail

Interaction partners 1. hen egg white lysozyme, : 7 interactors Detail

More details for 7 interactors identified by 6 citations

Experiments performed 6 experiments Detail

Chemical shift validation 3 contents Detail

Ptnr_site_1	Ptnr_site_2	Redox_state_prediction_1	Redox_state_prediction_2	Distance (Å)
1:7:CYS:SG	1:128:CYS:SG	unknown	unknown	n/a
1:31:CYS:SG	1:116:CYS:SG	unknown	unknown	n/a

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	Warning	593		84.6 (chain: 1, length: 130)
1	Chemical shift references	chemical_shift_reference	OK	1		No information

Comp_index_ID	Comp_ID	Atom_ID	CS value (ppm)
16	HIS	HD1	7.58

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	755	488	64.6

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	270	222	82.2

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	485	266	54.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	66	52	78.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	65	49	75.4

Keywords Disulfide Intermediate, hen Lysozyme, Protein Folding, Two-Disulfide Variant

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5069_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	True (see coordinates for details)
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

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Found 1 Document (1.404 seconds)

BMRB: 5069

Sample

Entries sharing articles BMRB: 6 entries Detail

Related entities 1. hen egg white lysozyme, : 1 : 105 : 141 entities Detail

Interaction partners 1. hen egg white lysozyme, : 7 interactors Detail

Experiments performed 6 experiments Detail

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Chemical shift validation 3 contents Detail