BMRB: 5071

Complex of the C-Domain of Troponin C with Residues 1-40 of Troponin I

Authors

Mercier, P., Spyracopoulos, L., Sykes, B.D.

Assembly

troponin C, skeletal muscle

Entity

1. TROPONIN C (polymer, Thiol state: all free), 77 monomers, 8874.749 Da Detail

MMEDAKGKSE EELANCFRIF DKNADGFIDI EELGEILRAT GEHVIEEDIE DLMKDSDKNN DGRIDFDEFL KMMEGVQ

2. TROPONIN I (polymer, Thiol state: not present), 40 monomers, 4678.206 Da Detail

GDEEKRNRAI TARRQHLKSV MLQIAATELE KEEGRREAEK

3. CA (non-polymer), 40.078 × 2 Da

Total weight

13633.111 Da

Max. entity weight

8874.749 Da

Source organism

Gallus gallus

Exptl. method

NMR

Refine. method

simmulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 65.0 %, Completeness: 51.5 %, Completeness (bb): 54.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H	13C	15N
All	51.5 % (702 of 1362)	54.6 % (394 of 721)	45.3 % (234 of 516)	59.2 % (74 of 125)
Backbone	54.0 % (379 of 702)	64.5 % (156 of 242)	43.4 % (149 of 343)	63.2 % (74 of 117)
Sidechain	51.0 % (392 of 769)	49.7 % (238 of 479)	54.6 % (154 of 282)	0.0 % (0 of 8)
Aromatic	5.2 % (3 of 58)	6.9 % (2 of 29)	3.4 % (1 of 29)
Methyl	63.5 % (66 of 104)	63.5 % (33 of 52)	63.5 % (33 of 52)

1. TROPONIN C

MMEDAKGKSE EELANCFRIF DKNADGFIDI EELGEILRAT GEHVIEEDIE DLMKDSDKNN DGRIDFDEFL KMMEGVQ

2. TROPONIN I

GDEEKRNRAI TARRQHLKSV MLQIAATELE KEEGRREAEK

Show sample condition

Sample

Pressure 1 atm, Temperature 303 K, pH 6.8

#	Name	Isotope labeling	Type	Concentration
1	TROPONIN C	[U-15N; U-13C]		1.3 mM
2	TROPONIN I			1.9 mM
3	KCl			100 mM
4	Imidazole			10 mM
5	DSS			1 mM
6	H2O			90 %
7	D2O			10 %

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LACS Plot; CA

Referencing offset: 0.42 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; CB

Referencing offset: 0.42 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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LACS Plot; HA

Referencing offset: 0.01 ppm, Outliers: 1 Detail

Show chemical shift referencing parameters

Chemical shift reference

Atom type	Mol. common name	Atom group	Value	Ref. method	Ref. type	Shift ratio
1H	DSS	methyl protons	0.0 ppm	null	direct	1.0
15N	DSS	methyl protons	0.0 ppm	null	indirect	0.1013291
13C	DSS	methyl protons	0.0 ppm	null	indirect	0.2514495

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Protein Blocks Logo

Calculated from 30 models in PDB: 1JC2, Strand ID: A Detail

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Release date

2001-11-13

Citation

Structure, dynamics, and thermodynamics of the structural domain of troponin C in complex with the regulatory peptide 1-40 of troponin I
Mercier, P., Spyracopoulos, L., Sykes, B.D.
Biochemistry (2001), 40, 10063-10077, PubMed 11513585 , DOI: , Abstract

Related entities 1. TROPONIN C, : 1 : 367 entities Detail

Related entities 2. TROPONIN I, : 2 : 2 : 13 entities Detail

Interaction partners 1. TROPONIN C, : 2 : 1 interactors Detail

Interaction partners 2. TROPONIN I, : 1 interactors Detail

Experiments performed 8 experiments Detail