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Found 1 Document (1.61 seconds)

BMRB: 5094

1K19

Chemosensory Protein from Moth Mamestra brassicae. Expression and Secondary Structure from 1H and 15N NMR

Authors

Campanacci, V., Mosbah, A., Bornet, O., Wechselberger, R., Jacquin-Joly, E., Cambillau, C., Darbon, H., Tegoni, M.

Assembly

chemosensory protein

Entity

1. chemosensory protein (polymer, Thiol state: all disulfide bound), 112 monomers, 13072.66 Da Detail

EDKYTDKYDN INLDEILANK RLLVAYVNCV MERGKCSPEG KELKEHLQDA IENGCKKCTE NQEKGAYRVI EHLIKNEIEI WRELTAKYDP TGNWRKKYED RAKAAGIVIP EE

Formula weight

13072.66 Da

Entity Connection

disulfide 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS29:SG	1:CYS36:SG
2	disulfide	sing	1:CYS55:SG	1:CYS58:SG

Source organism

Mamestra brassicae

Exptl. method

NMR

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 97.3 %, Completeness: 89.7 %, Completeness (bb): 96.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	89.7 % (747 of 833)	88.9 % (633 of 712)	94.2 % (114 of 121)
Backbone	96.4 % (324 of 336)	96.5 % (219 of 227)	96.3 % (105 of 109)
Sidechain	85.1 % (423 of 497)	85.4 % (414 of 485)	75.0 % (9 of 12)
Aromatic	97.6 % (41 of 42)	100.0 % (40 of 40)	50.0 % (1 of 2)
Methyl	89.3 % (50 of 56)	89.3 % (50 of 56)

1. chemosensory protein

EDKYTDKYDN INLDEILANK RLLVAYVNCV MERGKCSPEG KELKEHLQDA IENGCKKCTE NQEKGAYRVI EHLIKNEIEI WRELTAKYDP TGNWRKKYED RAKAAGIVIP EE

Show sample condition

Sample

Pressure 1 atm, Temperature 296 K, pH 6.9

#	Name	Concentration
1	chemosensory protein	2 mM
2	sodium phosphate buffer	2 mM
3	H2O	90 %
4	D2O	10 %

Protein Blocks Logo

Calculated from 20 models in PDB: 1K19, Strand ID: A Detail

Coupling constant

96 J values in 1 lists
Pressure 1 atm, Temperature 296 K, pH 6.9 Detail

Release date

2001-08-01

Citation

Chemosensory protein from the moth Mamestra brassicae. Expression and secondary structure from 1H and 15N NMR
Campanacci, V., Mosbah, A., Bornet, O., Wechselberger, R., Jacquin-Joly, E., Cambillau, C., Darbon, H., Tegoni, M.
Eur. J. Biochem. (2001), 268, 4731-4739, PubMed 11532009 , DOI: , Abstract

Related entities 1. chemosensory protein, : 1 : 4 : 11 entities Detail

Experiments performed 6 experiments Detail

1 2D NOESY

Instrument

Model Varian INOVA (750 MHz)

Sample #1

Pressure 1 atm, Temperature 296 K, pH 6.9

#	Name	Concentration
1	chemosensory protein	2 mM
2	sodium phosphate buffer	2 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 296 K, pH 6.9

#	Name	Isotope labeling	Concentration
5	chemosensory protein	[U-15N]	0.5 mM
6	sodium phosphate buffer		0.5 mM
7	H2O		90 %
8	D2O		10 %

2 DQF-COSY

Instrument

Model Varian INOVA (750 MHz)

Sample #1

Pressure 1 atm, Temperature 296 K, pH 6.9

#	Name	Concentration
1	chemosensory protein	2 mM
2	sodium phosphate buffer	2 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 296 K, pH 6.9

#	Name	Isotope labeling	Concentration
5	chemosensory protein	[U-15N]	0.5 mM
6	sodium phosphate buffer		0.5 mM
7	H2O		90 %
8	D2O		10 %

3 clean-TOCSY

Instrument

Model Varian INOVA (750 MHz)

Sample #1

Pressure 1 atm, Temperature 296 K, pH 6.9

#	Name	Concentration
1	chemosensory protein	2 mM
2	sodium phosphate buffer	2 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 296 K, pH 6.9

#	Name	Isotope labeling	Concentration
5	chemosensory protein	[U-15N]	0.5 mM
6	sodium phosphate buffer		0.5 mM
7	H2O		90 %
8	D2O		10 %

4 3D 15N-1H-HSQC NOESY

Instrument

Model Varian INOVA (750 MHz)

Sample #1

Pressure 1 atm, Temperature 296 K, pH 6.9

#	Name	Concentration
1	chemosensory protein	2 mM
2	sodium phosphate buffer	2 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 296 K, pH 6.9

#	Name	Isotope labeling	Concentration
5	chemosensory protein	[U-15N]	0.5 mM
6	sodium phosphate buffer		0.5 mM
7	H2O		90 %
8	D2O		10 %

5 3D 15N-1H-HSQC TOCSY

Instrument

Model Varian INOVA (750 MHz)

Sample #1

Pressure 1 atm, Temperature 296 K, pH 6.9

#	Name	Concentration
1	chemosensory protein	2 mM
2	sodium phosphate buffer	2 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 296 K, pH 6.9

#	Name	Isotope labeling	Concentration
5	chemosensory protein	[U-15N]	0.5 mM
6	sodium phosphate buffer		0.5 mM
7	H2O		90 %
8	D2O		10 %

6 2D 15N-1H-HSQC

Instrument

Model Varian INOVA (750 MHz)

Sample #1

Pressure 1 atm, Temperature 296 K, pH 6.9

#	Name	Concentration
1	chemosensory protein	2 mM
2	sodium phosphate buffer	2 mM
3	H2O	90 %
4	D2O	10 %

Sample #2

Pressure 1 atm, Temperature 296 K, pH 6.9

#	Name	Isotope labeling	Concentration
5	chemosensory protein	[U-15N]	0.5 mM
6	sodium phosphate buffer		0.5 mM
7	H2O		90 %
8	D2O		10 %

nullKeywords NMR, chemosensory protein, membrane, moth, pheromone

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Found 1 Document (1.61 seconds)

BMRB: 5094

Sample

Related entities 1. chemosensory protein, : 1 : 4 : 11 entities Detail

Experiments performed 6 experiments Detail

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