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Found 1 Document (1.591 seconds)

BMRB: 5100

1J2O

1H, 15N and 13C Assignments of FLIN2, an Intramolecular LMO2:ldb1 Complex

Authors

Matthews, J.M., Visvader, J.E., Mackay, J.P.

Assembly

Intramolecular complex of LMO2(LIM1) and ldb1(LID)

Entity

1. Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2 (polymer, Thiol state: free and other bound), 114 monomers, 12589.02 Da Detail

GSLLTCGGCQ QNIGDRYFLK AIDQYWHEDC LSCDLCGCRL GEVGRRLYYK LGRKLCRRDY LRLGGSGGHM GSGGDVMVVG EPTLMGGEFG DEDERLITRL ENTQFDAANG IDDE

2. ZN (non-polymer), 65.409 × 2 Da

Total weight

12719.838 Da

Max. entity weight

12589.02 Da

Entity Connection

metal coordination 8 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	metal coordination	sing	1:CYS6:SG	2:ZN1:ZN
2	metal coordination	sing	1:CYS9:SG	2:ZN1:ZN
3	metal coordination	sing	1:HIS27:ND1	2:ZN1:ZN
4	metal coordination	sing	1:CYS30:SG	2:ZN1:ZN
5	metal coordination	sing	1:CYS33:SG	2:ZN1:ZN
6	metal coordination	sing	1:CYS36:SG	2:ZN1:ZN
7	metal coordination	sing	1:CYS56:SG	2:ZN1:ZN
8	metal coordination	sing	1:ASP59:OD1	2:ZN1:ZN

Source organism

Mus musculus

Exptl. method

NMR

Refine. method

distance geometry, simulated annealing, molecular dynamics, automated noe assignment of ambiguous NOES

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 97.4 %, Completeness: 80.5 %, Completeness (bb): 94.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	80.5 % (1011 of 1256)	81.0 % (530 of 654)	76.3 % (367 of 481)	94.2 % (114 of 121)
Backbone	94.6 % (645 of 682)	94.7 % (234 of 247)	94.4 % (304 of 322)	94.7 % (107 of 113)
Sidechain	68.4 % (457 of 668)	72.7 % (296 of 407)	60.9 % (154 of 253)	87.5 % (7 of 8)
Aromatic	11.1 % (10 of 90)	22.2 % (10 of 45)	0.0 % (0 of 44)	0.0 % (0 of 1)
Methyl	62.7 % (64 of 102)	76.5 % (39 of 51)	49.0 % (25 of 51)

1. Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2

GSLLTCGGCQ QNIGDRYFLK AIDQYWHEDC LSCDLCGCRL GEVGRRLYYK LGRKLCRRDY LRLGGSGGHM GSGGDVMVVG EPTLMGGEFG DEDERLITRL ENTQFDAANG IDDE

Show sample condition

Sample #1

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 15N]		0.3 ~ 0.5 mM
4	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
5	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 13C; U-90% 15N]		0.3 ~ 0.5 mM
6	ZINC (II) ION			0.6 ~ 1.0 mM

Chem. Shift Complete2

Sequence coverage: 100.0 %, Completeness: 69.1 %, Completeness (bb): 70.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	69.1 % (1737 of 2512)	89.2 % (1167 of 1308)	44.5 % (428 of 962)	58.7 % (142 of 242)
Backbone	70.7 % (964 of 1364)	97.0 % (479 of 494)	54.7 % (352 of 644)	58.8 % (133 of 226)
Sidechain	65.7 % (878 of 1336)	84.5 % (688 of 814)	35.8 % (181 of 506)	56.3 % (9 of 16)
Aromatic	29.4 % (53 of 180)	56.7 % (51 of 90)	2.3 % (2 of 88)	0.0 % (0 of 2)
Methyl	58.8 % (120 of 204)	86.3 % (88 of 102)	31.4 % (32 of 102)

1. Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2

GSLLTCGGCQ QNIGDRYFLK AIDQYWHEDC LSCDLCGCRL GEVGRRLYYK LGRKLCRRDY LRLGGSGGHM GSGGDVMVVG EPTLMGGEFG DEDERLITRL ENTQFDAANG IDDE

Show sample condition

Sample

Temperature 298 (±0.5) K, pH 5.6 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2			0.3 ~ 0.5 mM
2	ZINC (II) ION			0.6 ~ 1.0 mM

Protein Blocks Logo

Calculated from 20 models in PDB: 1J2O, Strand ID: A Detail

Release date

2002-04-04

Citation 1

1H, 15N and 13C assignments of FLIN2, an intramolecular LMO2:ldb1 complex
Matthews, J.M., Visvader, J.E., Mackay, J.P.
J. Biomol. NMR (2001), 21, 385-386, PubMed 11824760 , DOI: , Abstract

Show more 1 citaion

Citation 2

Design, production and characterization of FLIN2 and FLIN4: the engineering of intramolecular ldb1:LMO complexes
Deane, J.E., Sum, E., Mackay, J.P., Lindeman, G.J., Visvader, J.E., Matthews, J.M.
Protein Eng. (2001), 14, 493-499, PubMed 11522923 , DOI: , Abstract

Hide non-primary 1 citaion

Related entities 1. Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2, : 1 : 13 entities Detail

Experiments performed 14 experiments Detail

1 1H-1H TOCSY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
1	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2			0.3 ~ 0.5 mM
2	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
3	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 15N]		0.3 ~ 0.5 mM
4	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #3

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
5	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 13C; U-90% 15N]		0.3 ~ 0.5 mM
6	ZINC (II) ION			0.6 ~ 1.0 mM

2 1H-1H COSY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
1	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2			0.3 ~ 0.5 mM
2	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
3	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 15N]		0.3 ~ 0.5 mM
4	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #3

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
5	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 13C; U-90% 15N]		0.3 ~ 0.5 mM
6	ZINC (II) ION			0.6 ~ 1.0 mM

3 1H-1H NOESY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
1	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2			0.3 ~ 0.5 mM
2	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
3	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 15N]		0.3 ~ 0.5 mM
4	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #3

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
5	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 13C; U-90% 15N]		0.3 ~ 0.5 mM
6	ZINC (II) ION			0.6 ~ 1.0 mM

4 HNCA

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
1	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2			0.3 ~ 0.5 mM
2	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
3	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 15N]		0.3 ~ 0.5 mM
4	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #3

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
5	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 13C; U-90% 15N]		0.3 ~ 0.5 mM
6	ZINC (II) ION			0.6 ~ 1.0 mM

5 HN(CO)CA

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
1	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2			0.3 ~ 0.5 mM
2	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
3	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 15N]		0.3 ~ 0.5 mM
4	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #3

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
5	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 13C; U-90% 15N]		0.3 ~ 0.5 mM
6	ZINC (II) ION			0.6 ~ 1.0 mM

6 HNCACB

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
1	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2			0.3 ~ 0.5 mM
2	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
3	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 15N]		0.3 ~ 0.5 mM
4	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #3

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
5	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 13C; U-90% 15N]		0.3 ~ 0.5 mM
6	ZINC (II) ION			0.6 ~ 1.0 mM

7 CBCA(CO)NH

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
1	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2			0.3 ~ 0.5 mM
2	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
3	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 15N]		0.3 ~ 0.5 mM
4	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #3

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
5	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 13C; U-90% 15N]		0.3 ~ 0.5 mM
6	ZINC (II) ION			0.6 ~ 1.0 mM

8 CC(CO)NH TOCSY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
1	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2			0.3 ~ 0.5 mM
2	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
3	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 15N]		0.3 ~ 0.5 mM
4	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #3

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
5	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 13C; U-90% 15N]		0.3 ~ 0.5 mM
6	ZINC (II) ION			0.6 ~ 1.0 mM

9 HNCO

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
1	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2			0.3 ~ 0.5 mM
2	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
3	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 15N]		0.3 ~ 0.5 mM
4	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #3

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
5	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 13C; U-90% 15N]		0.3 ~ 0.5 mM
6	ZINC (II) ION			0.6 ~ 1.0 mM

10 HN(CA)CO

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
1	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2			0.3 ~ 0.5 mM
2	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
3	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 15N]		0.3 ~ 0.5 mM
4	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #3

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
5	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 13C; U-90% 15N]		0.3 ~ 0.5 mM
6	ZINC (II) ION			0.6 ~ 1.0 mM

11 HNHA

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
1	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2			0.3 ~ 0.5 mM
2	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
3	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 15N]		0.3 ~ 0.5 mM
4	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #3

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
5	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 13C; U-90% 15N]		0.3 ~ 0.5 mM
6	ZINC (II) ION			0.6 ~ 1.0 mM

12 1H-15N TOCSY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
1	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2			0.3 ~ 0.5 mM
2	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
3	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 15N]		0.3 ~ 0.5 mM
4	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #3

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
5	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 13C; U-90% 15N]		0.3 ~ 0.5 mM
6	ZINC (II) ION			0.6 ~ 1.0 mM

13 1H-15N NOESY

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
1	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2			0.3 ~ 0.5 mM
2	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
3	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 15N]		0.3 ~ 0.5 mM
4	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #3

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
5	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 13C; U-90% 15N]		0.3 ~ 0.5 mM
6	ZINC (II) ION			0.6 ~ 1.0 mM

14 1H-15N HSQC

Instrument

Model Bruker DMX (600 MHz)

Sample #1

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
1	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2			0.3 ~ 0.5 mM
2	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #2

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
3	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 15N]		0.3 ~ 0.5 mM
4	ZINC (II) ION			0.6 ~ 1.0 mM

Sample #3

Temperature 298 (±0.5) K

#	Name	Isotope labeling	Type	Concentration
5	Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2	[U-95% 13C; U-90% 15N]		0.3 ~ 0.5 mM
6	ZINC (II) ION			0.6 ~ 1.0 mM

nullKeywords FLIN2, ldb1, LMO2

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Found 1 Document (1.591 seconds)

BMRB: 5100

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Related entities 1. Fusion of the LIM interaction domain of ldb1 and the N-terminal LIM domain of LMO2, : 1 : 13 entities Detail

Experiments performed 14 experiments Detail

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