BMRBj - BMREntryMaster

Found 1 Document (1.703 seconds)

BMRB: 5101

1GJZ

Structure and Properties of a Dimeric N-terminal Fragment of Human Ubiquitin

Authors

Bolton, D., Evans, P.A., Stott, K., Broadhurst, R.W.

Assembly

ubiquitin fragment

Entity

1. ubiquitin fragment (polymer, Thiol state: not present), 53 monomers, 5884.703 × 2 Da Detail

GSMQIFVKTL TGKTITLEVE PSDTIENVKA KIQDKEGIPP DQQRLIFAGK QLE

Total weight

11769.406 Da

Max. entity weight

5884.703 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

SIMULATED ANNEALING

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 86.6 %, Completeness (bb): 80.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	86.6 % (549 of 634)	96.4 % (322 of 334)	73.0 % (178 of 244)	87.5 % (49 of 56)
Backbone	80.1 % (250 of 312)	98.1 % (105 of 107)	62.6 % (97 of 155)	96.0 % (48 of 50)
Sidechain	90.0 % (334 of 371)	93.4 % (212 of 227)	88.4 % (122 of 138)	0.0 % (0 of 6)
Aromatic	90.0 % (18 of 20)	90.0 % (9 of 10)	90.0 % (9 of 10)
Methyl	93.9 % (62 of 66)	93.9 % (31 of 33)	93.9 % (31 of 33)

1. Ubiquitin

GSMQIFVKTL TGKTITLEVE PSDTIENVKA KIQDKEGIPP DQQRLIFAGK QLE

Show sample condition

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin	[U-100% 13C; U-100% 15N]		1.0 mM
2	sodium sulphate			0.8 M

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin			1.0 mM
4	sodium sulphate			0.8 M

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.1 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.06 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 16 models in PDB: 1GJZ, Strand ID: A, B Detail

Release date

2002-01-24

Citation

Structure and properties of a dimeric N-terminal fragment of human ubiquitin
Bolton, D., Evans, P.A., Stott, K., Broadhurst, R.W.
J. Mol. Biol. (2001), 314, 773-787, PubMed 11733996 , DOI 10.1006/jmbi.2001.5181 , Abstract

Related entities 1. ubiquitin fragment, : 1 : 21 : 386 : 305 entities Detail

Interaction partners 1. ubiquitin fragment, : 5 interactors Detail

More details for 5 interactors identified by 4 citations

Experiments performed 7 experiments Detail

1 3D 1H-1H-15N NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin	[U-100% 13C; U-100% 15N]		1.0 mM
2	sodium sulphate			0.8 M

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin			1.0 mM
4	sodium sulphate			0.8 M

2 3D 1H-1H-15N TOCSY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin	[U-100% 13C; U-100% 15N]		1.0 mM
2	sodium sulphate			0.8 M

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin			1.0 mM
4	sodium sulphate			0.8 M

3 3D 13C-1H-1H NOESY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin	[U-100% 13C; U-100% 15N]		1.0 mM
2	sodium sulphate			0.8 M

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin			1.0 mM
4	sodium sulphate			0.8 M

4 3D 1H-13C-1H HCCH-TOCSY

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin	[U-100% 13C; U-100% 15N]		1.0 mM
2	sodium sulphate			0.8 M

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin			1.0 mM
4	sodium sulphate			0.8 M

5 3D CBCA(CO)NH

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin	[U-100% 13C; U-100% 15N]		1.0 mM
2	sodium sulphate			0.8 M

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin			1.0 mM
4	sodium sulphate			0.8 M

6 3D HNCA

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin	[U-100% 13C; U-100% 15N]		1.0 mM
2	sodium sulphate			0.8 M

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin			1.0 mM
4	sodium sulphate			0.8 M

7 3D HN(CO)CA

Instrument

Model Bruker DRX (500 MHz)

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	Ubiquitin	[U-100% 13C; U-100% 15N]		1.0 mM
2	sodium sulphate			0.8 M

Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1) K, pH 7.0 (±0.2)

#	Name	Isotope labeling	Type	Concentration
3	Ubiquitin			1.0 mM
4	sodium sulphate			0.8 M

nullKeywords ubiquitin, dimer, protein dissection, structural specificity, NMR spectroscopy

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Found 1 Document (1.703 seconds)

BMRB: 5101

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Chemical shift reference

Chemical shift reference

Chemical shift reference

Related entities 1. ubiquitin fragment, : 1 : 21 : 386 : 305 entities Detail

Interaction partners 1. ubiquitin fragment, : 5 interactors Detail

Experiments performed 7 experiments Detail

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