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Structure and Properties of a Dimeric N-terminal Fragment of Human Ubiquitin
Authors
Bolton, D., Evans, P.A., Stott, K., Broadhurst, R.W.
Assembly
ubiquitin fragment
Entity
1. ubiquitin fragment (polymer, Thiol state: not present), 53 monomers, 5884.703 × 2 Da Detail

GSMQIFVKTL TGKTITLEVE PSDTIENVKA KIQDKEGIPP DQQRLIFAGK QLE


Total weight
11769.406 Da
Max. entity weight
5884.703 Da
Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
SIMULATED ANNEALING
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 86.3 %, Completeness (bb): 80.2 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All86.3 % (548 of 635)96.4 % (322 of 334)72.5 % (177 of 244)86.0 % (49 of 57)
Backbone80.2 % (247 of 308)99.0 % (102 of 103)61.9 % (96 of 155)98.0 % (49 of 50)
Sidechain92.3 % (347 of 376)95.2 % (220 of 231)92.0 % (127 of 138) 0.0 % (0 of 7)
Aromatic90.0 % (18 of 20)90.0 % (9 of 10)90.0 % (9 of 10)
Methyl100.0 % (66 of 66)100.0 % (33 of 33)100.0 % (33 of 33)

1. Ubiquitin

GSMQIFVKTL TGKTITLEVE PSDTIENVKA KIQDKEGIPP DQQRLIFAGK QLE

Sample #1

Pressure 1 (±0.1) atm, Temperature 298 (±1) K, pH 7.0 (±0.2)


#NameIsotope labelingTypeConcentration
1Ubiquitin[U-100% 13C; U-100% 15N]1.0 mM
2sodium sulphate0.8 M
Sample #2

Pressure 1 (±0.1) atm, Temperature 298 (±1) K, pH 7.0 (±0.2)


#NameIsotope labelingTypeConcentration
3Ubiquitin1.0 mM
4sodium sulphate0.8 M

LACS Plot; CA
Referencing offset: -0.1 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.1 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.06 ppm, Outliers: 1 Detail
Protein Blocks Logo
Calculated from 16 models in PDB: 1GJZ, Strand ID: A, B Detail


Release date
2002-01-24
Citation
Structure and properties of a dimeric N-terminal fragment of human ubiquitin
Bolton, D., Evans, P.A., Stott, K., Broadhurst, R.W.
J. Mol. Biol. (2001), 314, 773-787, PubMed 11733996 , DOI 10.1006/jmbi.2001.5181 ,
Related entities 1. ubiquitin fragment, : 1 : 21 : 238 : 132 entities Detail
Interaction partners 1. ubiquitin fragment, : 5 interactors Detail
Experiments performed 7 experiments Detail
Keywords ubiquitin, dimer, protein dissection, structural specificity, NMR spectroscopy