Search

Characterization of the Structure and Dynamics of Amyloidogenic Variants of Human Lysozyme by NMR Spectroscopy
Authors
Chamberlain, A.K., Receveur, V., Spencer, A., Redfield, C., Dobson, C.M.
Assembly
Human Lysozyme I56T
Entity
1. Human Lysozyme I56T (polymer, Thiol state: not reported), 130 monomers, 14688.47 Da Detail

KVFERCELAR TLKRLGMDGY RGISLANWMC LAKWESGYNT RATNYNAGDR STDYGTFQIN SRYWCNDGKT PGAVNACHLS CSALLQDNIA DAVACAKRVV RDPQGIRAWV AWRNRCQNRD VRQYVQGCGV


Formula weight
14688.47 Da
Source organism
Homo sapiens
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 97.7 %, Completeness: 30.0 %, Completeness (bb): 66.5 % Detail

Polymer type: polypeptide(L)

Total1H15N
All30.0 % (275 of 917)19.1 % (147 of 768)85.9 % (128 of 149)
Backbone66.5 % (264 of 397)50.9 % (137 of 269)99.2 % (127 of 128)
Sidechain 2.1 % (11 of 520) 2.0 % (10 of 499) 4.8 % (1 of 21)
Aromatic 0.0 % (0 of 71) 0.0 % (0 of 66) 0.0 % (0 of 5)
Methyl 1.6 % (1 of 62) 1.6 % (1 of 62)

1. lysozyme

KVFERCELAR TLKRLGMDGY RGISLANWMC LAKWESGYNT RATNYNAGDR STDYGTFQIN SRYWCNDGKT PGAVNACHLS CSALLQDNIA DAVACAKRVV RDPQGIRAWV AWRNRCQNRD VRQYVQGCGV

Sample

Temperature 310 (±1) K, pH 5.0 (±0.2)


#NameIsotope labelingTypeConcentration
1lysozyme0.8 ~ 1.2 mM
2HCl0.0 ~ 0.0 mM
3H2O100 %

Release date
2002-01-24
Citation
Characterization of the structure and dynamics of amyloidogenic variants of human lysozyme by NMR spectroscopy
Chamberlain, A.K., Receveur, V., Spencer, A., Redfield, C., Dobson, C.M.
Protein Sci. (2001), 10, 2525-2530, PubMed 11714920 , DOI 10.1110/ps.28101 ,
Entries sharing articles BMRB: 2 entries Detail
  BMRB: 5123 released on 2002-01-24
    Title Characterization of the Structure and Dynamics of Amyloidogenic Variants of Human Lysozyme by NMR Spectroscopy
  BMRB: 5125 released on 2002-01-24
    Title Characterization of the Structure and Dynamics of Amyloidogenic Variants of Human Lysozyme by NMR Spectroscopy
Related entities 1. Human Lysozyme I56T, : 1 : 2 : 64 : 181 entities Detail
Interaction partners 1. Human Lysozyme I56T, : 41 interactors Detail
Experiments performed 2 experiments Detail
Chemical shift validation 3 contents Detail
Keywords amyloid fibrils, backbone dynamics, lysozyme, mutant, NMR