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Characterization of the Structure and Dynamics of Amyloidogenic Variants of Human Lysozyme by NMR Spectroscopy
Authors
Chamberlain, A.K., Receveur, V., Spencer, A., Redfield, C., Dobson, C.M.
Assembly
Human Lysozyme D67H
Entity
1. Human Lysozyme D67H (polymer, Thiol state: not reported), 130 monomers, 14722.58 Da Detail

KVFERCELAR TLKRLGMDGY RGISLANWMC LAKWESGYNT RATNYNAGDR STDYGIFQIN SRYWCNHGKT PGAVNACHLS CSALLQDNIA DAVACAKRVV RDPQGIRAWV AWRNRCQNRD VRQYVQGCGV


Formula weight
14722.58 Da
Source organism
Homo sapiens
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 96.2 %, Completeness: 28.5 %, Completeness (bb): 63.2 % Detail

Polymer type: polypeptide(L)

Total1H15N
All28.5 % (267 of 936)18.4 % (142 of 773)76.7 % (125 of 163)
Backbone63.2 % (244 of 386)46.1 % (119 of 258)97.7 % (125 of 128)
Sidechain 4.2 % (23 of 550) 4.5 % (23 of 515) 0.0 % (0 of 35)
Aromatic 0.0 % (0 of 73) 0.0 % (0 of 68) 0.0 % (0 of 5)
Methyl 3.2 % (2 of 63) 3.2 % (2 of 63)

1. lysozyme

KVFERCELAR TLKRLGMDGY RGISLANWMC LAKWESGYNT RATNYNAGDR STDYGIFQIN SRYWCNHGKT PGAVNACHLS CSALLQDNIA DAVACAKRVV RDPQGIRAWV AWRNRCQNRD VRQYVQGCGV

Sample

Temperature 310 (±1) K, pH 5.0 (±0.2)


#NameIsotope labelingTypeConcentration
1lysozyme0.8 ~ 1.2 mM
2HCl0.0 ~ 0.0 mM
3H2O100 %

Release date
2002-01-24
Citation
Characterization of the structure and dynamics of amyloidogenic variants of human lysozyme by NMR spectroscopy
Chamberlain, A.K., Receveur, V., Spencer, A., Redfield, C., Dobson, C.M.
Protein Sci. (2001), 10, 2525-2530, PubMed 11714920 , DOI 10.1110/ps.28101 ,
Related entities 1. Human Lysozyme D67H, : 1 : 62 : 140 entities Detail
Interaction partners 1. Human Lysozyme D67H, : 43 interactors Detail
Experiments performed 3 experiments Detail
Keywords amyloid fibrils, backbone dynamics, lysozyme, mutant, NMR