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Characterization of the Structure and Dynamics of Amyloidogenic Variants of Human Lysozyme by NMR Spectroscopy
Authors
Chamberlain, A.K., Receveur, V., Spencer, A., Redfield, C., Dobson, C.M.
Assembly
Human Lysozyme D67H
Entity
1. Human Lysozyme D67H (polymer, Thiol state: not reported), 130 monomers, 14722.58 Da Detail

KVFERCELAR TLKRLGMDGY RGISLANWMC LAKWESGYNT RATNYNAGDR STDYGIFQIN SRYWCNHGKT PGAVNACHLS CSALLQDNIA DAVACAKRVV RDPQGIRAWV AWRNRCQNRD VRQYVQGCGV


Formula weight
14722.58 Da
Source organism
Homo sapiens
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 95.4 %, Completeness: 30.0 %, Completeness (bb): 64.7 % Detail

Polymer type: polypeptide(L)

Total1H15N
All30.0 % (277 of 922)19.8 % (153 of 773)83.2 % (124 of 149)
Backbone64.7 % (257 of 397)49.4 % (133 of 269)96.9 % (124 of 128)
Sidechain 4.2 % (22 of 525) 4.4 % (22 of 504) 0.0 % (0 of 21)
Aromatic 0.0 % (0 of 73) 0.0 % (0 of 68) 0.0 % (0 of 5)
Methyl 3.2 % (2 of 63) 3.2 % (2 of 63)

1. lysozyme

KVFERCELAR TLKRLGMDGY RGISLANWMC LAKWESGYNT RATNYNAGDR STDYGIFQIN SRYWCNHGKT PGAVNACHLS CSALLQDNIA DAVACAKRVV RDPQGIRAWV AWRNRCQNRD VRQYVQGCGV

Sample

Temperature 310 (±1) K, pH 5.0 (±0.2)


#NameIsotope labelingTypeConcentration
1lysozyme0.8 ~ 1.2 mM
2HCl0.0 ~ 0.0 mM
3H2O100 %

Release date
2002-01-24
Citation
Characterization of the structure and dynamics of amyloidogenic variants of human lysozyme by NMR spectroscopy
Chamberlain, A.K., Receveur, V., Spencer, A., Redfield, C., Dobson, C.M.
Protein Sci. (2001), 10, 2525-2530, PubMed 11714920 , DOI 10.1110/ps.28101 ,
Entries sharing articles BMRB: 2 entries Detail
  BMRB: 5123 released on 2002-01-24
    Title Characterization of the Structure and Dynamics of Amyloidogenic Variants of Human Lysozyme by NMR Spectroscopy
  BMRB: 5124 released on 2002-01-24
    Title Characterization of the Structure and Dynamics of Amyloidogenic Variants of Human Lysozyme by NMR Spectroscopy
Related entities 1. Human Lysozyme D67H, : 1 : 63 : 184 entities Detail
Interaction partners 1. Human Lysozyme D67H, : 41 interactors Detail
Experiments performed 3 experiments Detail
Chemical shift validation 3 contents Detail
Keywords amyloid fibrils, backbone dynamics, lysozyme, mutant, NMR