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Assignment of 1H, 13C and 15N resonances of Human Lysozyme at 35 C
Authors
Kumeta, H.
Assembly
human lysozyme at 35 degree C
Entity
1. human lysozyme at 35 degree C (polymer, Thiol state: all disulfide bound), 130 monomers, 14700.53 Da Detail

KVFERCELAR TLKRLGMDGY RGISLANWMC LAKWESGYNT RATNYNAGDR STDYGIFQIN SRYWCNDGKT PGAVNACHLS CSALLQDNIA DAVACAKRVV RDPQGIRAWV AWRNRCQNRD VRQYVQGCGV


Formula weight
14700.53 Da
Entity Connection
disulfide 4 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing0:CYS6:SG1:CYS128:SG
2disulfidesing0:CYS30:SG1:CYS116:SG
3disulfidesing0:CYS65:SG1:CYS81:SG
4disulfidesing0:CYS77:SG1:CYS95:SG

Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
distance geometry, simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 67.4 %, Completeness (bb): 82.5 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All67.4 % (1009 of 1496)79.2 % (611 of 771)48.4 % (272 of 562)77.3 % (126 of 163)
Backbone82.5 % (631 of 765)97.3 % (251 of 258)67.5 % (256 of 379)96.9 % (124 of 128)
Sidechain56.9 % (484 of 850)69.0 % (354 of 513)42.7 % (129 of 302) 2.9 % (1 of 35)
Aromatic 8.3 % (11 of 132)13.6 % (9 of 66) 3.3 % (2 of 61) 0.0 % (0 of 5)
Methyl64.3 % (81 of 126)100.0 % (63 of 63)28.6 % (18 of 63)

1. human lysozyme

KVFERCELAR TLKRLGMDGY RGISLANWMC LAKWESGYNT RATNYNAGDR STDYGIFQIN SRYWCNDGKT PGAVNACHLS CSALLQDNIA DAVACAKRVV RDPQGIRAWV AWRNRCQNRD VRQYVQGCGV

Sample

Temperature 308 (±1) K


#NameIsotope labelingTypeConcentration
1human lysozyme2 mM

LACS Plot; CA
Referencing offset: -0.17 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.17 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.05 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 1 models in PDB: 1IY4, Strand ID: A Detail


Release date
2001-11-11
Citation
Assignment of 1H, 13C and 15N resonances of Human Lysozyme at 35 and 4 degree C
Kumeta, H.
Related entities 1. human lysozyme at 35 degree C, : 1 : 34 : 43 : 25 : 100 entities Detail
Interaction partners 1. human lysozyme at 35 degree C, : 43 interactors Detail
Experiments performed 7 experiments Detail
Keywords human lysozyme