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Assignment of 1H, 13C and 15N resonances of Human Lysozyme at 35 C
Authors
Kumeta, H.
Assembly
human lysozyme at 35 degree C
Entity
1. human lysozyme at 35 degree C (polymer, Thiol state: all disulfide bound), 130 monomers, 14700.53 Da Detail

KVFERCELAR TLKRLGMDGY RGISLANWMC LAKWESGYNT RATNYNAGDR STDYGIFQIN SRYWCNDGKT PGAVNACHLS CSALLQDNIA DAVACAKRVV RDPQGIRAWV AWRNRCQNRD VRQYVQGCGV


Formula weight
14700.53 Da
Entity Connection
disulfide 4 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS6:SG1:CYS128:SG
2disulfidesing1:CYS30:SG1:CYS116:SG
3disulfidesing1:CYS65:SG1:CYS81:SG
4disulfidesing1:CYS77:SG1:CYS95:SG

Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
distance geometry, simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 69.4 %, Completeness (bb): 84.0 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All69.4 % (1028 of 1482)80.5 % (621 of 771)50.0 % (281 of 562)84.6 % (126 of 149)
Backbone84.0 % (652 of 776)98.9 % (266 of 269)68.6 % (260 of 379)98.4 % (126 of 128)
Sidechain60.0 % (495 of 825)70.7 % (355 of 502)46.4 % (140 of 302) 0.0 % (0 of 21)
Aromatic 6.1 % (8 of 132) 6.1 % (4 of 66) 6.6 % (4 of 61) 0.0 % (0 of 5)
Methyl66.7 % (84 of 126)100.0 % (63 of 63)33.3 % (21 of 63)

1. human lysozyme

KVFERCELAR TLKRLGMDGY RGISLANWMC LAKWESGYNT RATNYNAGDR STDYGIFQIN SRYWCNDGKT PGAVNACHLS CSALLQDNIA DAVACAKRVV RDPQGIRAWV AWRNRCQNRD VRQYVQGCGV

Sample

Temperature 308 (±1) K


#NameIsotope labelingTypeConcentration
1human lysozyme2 mM

LACS Plot; CA
Referencing offset: -0.17 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.17 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.05 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 1 models in PDB: 1IY4, Strand ID: A Detail


Release date
2001-09-05
Citation
Assignment of 1H, 13C and 15N resonances of Human Lysozyme at 35 and 4 degree C
Kumeta, H.
Related entities 1. human lysozyme at 35 degree C, : 1 : 39 : 39 : 25 : 144 entities Detail
Interaction partners 1. human lysozyme at 35 degree C, : 41 interactors Detail
Experiments performed 7 experiments Detail
nullKeywords human lysozyme