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Assignment of 1H, 13C and 15N resonances of Human Lysozyme at 4 C
Authors
Kumeta, H., Kobashigawa, Y., Miura, K., Nishimiya, Y., Oka, C., Nemoto, N., Miura, A., Nitta, K., Tsuda, S.
Assembly
human lysozyme
Entity
1. human lysozyme (polymer, Thiol state: all disulfide bound), 130 monomers, 14700.53 Da Detail

KVFERCELAR TLKRLGMDGY RGISLANWMC LAKWESGYNT RATNYNAGDR STDYGIFQIN SRYWCNDGKT PGAVNACHLS CSALLQDNIA DAVACAKRVV RDPQGIRAWV AWRNRCQNRD VRQYVQGCGV


Formula weight
14700.53 Da
Entity Connection
disulfide 4 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing1:CYS6:SG1:CYS128:SG
2disulfidesing1:CYS30:SG1:CYS116:SG
3disulfidesing1:CYS65:SG1:CYS81:SG
4disulfidesing1:CYS77:SG1:CYS95:SG

Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
distance geometry, simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 76.4 %, Completeness (bb): 98.3 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All76.4 % (1132 of 1482)79.1 % (610 of 771)70.1 % (394 of 562)85.9 % (128 of 149)
Backbone98.3 % (763 of 776)98.5 % (265 of 269)97.9 % (371 of 379)99.2 % (127 of 128)
Sidechain58.2 % (480 of 825)68.1 % (342 of 502)45.4 % (137 of 302) 4.8 % (1 of 21)
Aromatic13.6 % (18 of 132)22.7 % (15 of 66) 3.3 % (2 of 61)20.0 % (1 of 5)
Methyl64.3 % (81 of 126)96.8 % (61 of 63)31.7 % (20 of 63)

1. human lysozyme

KVFERCELAR TLKRLGMDGY RGISLANWMC LAKWESGYNT RATNYNAGDR STDYGIFQIN SRYWCNDGKT PGAVNACHLS CSALLQDNIA DAVACAKRVV RDPQGIRAWV AWRNRCQNRD VRQYVQGCGV

Sample

Temperature 277 (±1) K, pH 3.8 (±0.1)


#NameIsotope labelingTypeConcentration
1human lysozyme1.0 mM

LACS Plot; CA
Referencing offset: -0.39 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.39 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.03 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: -0.24 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 1 models in PDB: 1IY3, Strand ID: A Detail


Release date
2001-09-06
Citation
Assignments of 1H, 13C, and 15N resonances of human lysozyme at 4 degrees C
Kumeta, H., Kobashigawa, Y., Miura, K., Nishimiya, Y., Oka, C., Nemoto, N., Miura, A., Nitta, K., Tsuda, S.
J. Biomol. NMR (2002), 22, 183-184, PubMed 11883780 ,
Related entities 1. human lysozyme, : 1 : 39 : 39 : 25 : 144 entities Detail
Interaction partners 1. human lysozyme, : 41 interactors Detail
Experiments performed 7 experiments Detail
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