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Assignment of 1H, 13C and 15N resonances of Human Lysozyme at 4 C
Authors
Kumeta, H., Kobashigawa, Y., Miura, K., Nishimiya, Y., Oka, C., Nemoto, N., Miura, A., Nitta, K., Tsuda, S.
Assembly
human lysozyme
Entity
1. human lysozyme (polymer, Thiol state: all disulfide bound), 130 monomers, 14700.53 Da Detail

KVFERCELAR TLKRLGMDGY RGISLANWMC LAKWESGYNT RATNYNAGDR STDYGIFQIN SRYWCNDGKT PGAVNACHLS CSALLQDNIA DAVACAKRVV RDPQGIRAWV AWRNRCQNRD VRQYVQGCGV


Formula weight
14700.53 Da
Entity Connection
disulfide 4 Detail

IDTypeValue orderAtom ID 1Atom ID 2
1disulfidesing0:CYS6:SG1:CYS128:SG
2disulfidesing0:CYS30:SG1:CYS116:SG
3disulfidesing0:CYS65:SG1:CYS81:SG
4disulfidesing0:CYS77:SG1:CYS95:SG

Source organism
Homo sapiens
Exptl. method
NMR
Refine. method
distance geometry, simulated annealing
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 74.2 %, Completeness (bb): 98.4 % Detail

Polymer type: polypeptide(L)

Total1H13C15N
All74.2 % (1110 of 1496)77.6 % (598 of 771)68.3 % (384 of 562)78.5 % (128 of 163)
Backbone98.4 % (753 of 765)98.1 % (253 of 258)98.7 % (374 of 379)98.4 % (126 of 128)
Sidechain55.9 % (475 of 850)67.3 % (345 of 513)42.4 % (128 of 302) 5.7 % (2 of 35)
Aromatic 0.0 % (0 of 132) 0.0 % (0 of 66) 0.0 % (0 of 61) 0.0 % (0 of 5)
Methyl63.5 % (80 of 126)98.4 % (62 of 63)28.6 % (18 of 63)

1. human lysozyme

KVFERCELAR TLKRLGMDGY RGISLANWMC LAKWESGYNT RATNYNAGDR STDYGIFQIN SRYWCNDGKT PGAVNACHLS CSALLQDNIA DAVACAKRVV RDPQGIRAWV AWRNRCQNRD VRQYVQGCGV

Sample

Temperature 277 (±1) K, pH 3.8 (±0.1)


#NameIsotope labelingTypeConcentration
1human lysozyme1.0 mM

LACS Plot; CA
Referencing offset: -0.39 ppm, Outliers: 1 Detail
LACS Plot; CB
Referencing offset: -0.39 ppm, Outliers: 1 Detail
LACS Plot; HA
Referencing offset: -0.03 ppm, Outliers: 2 Detail
LACS Plot; CO
Referencing offset: -0.24 ppm, Outliers: 2 Detail
Protein Blocks Logo
Calculated from 1 models in PDB: 1IY3, Strand ID: A Detail


Release date
2002-05-05
Citation
Assignments of 1H, 13C, and 15N resonances of human lysozyme at 4 degrees C
Kumeta, H., Kobashigawa, Y., Miura, K., Nishimiya, Y., Oka, C., Nemoto, N., Miura, A., Nitta, K., Tsuda, S.
J. Biomol. NMR (2002), 22, 183-184, PubMed 11883780
Related entities 1. human lysozyme, : 1 : 34 : 43 : 25 : 100 entities Detail
Interaction partners 1. human lysozyme, : 43 interactors Detail
Experiments performed 7 experiments Detail