BMRB: 5144

NMR Structure of the Second Intracellular Loop of the alpha2A adrenergic receptor: evidence for a Novel Cytoplasmic Helix

Authors

Chung, D.A., Zuiderweg, E.R.P., Fowler, C.B., Soyer, O.S., Mosberg, H.I., Neubig, R.R.

Assembly

rhodopsin

Entity

1. rhodopsin (polymer, Thiol state: all free), 34 monomers, 3850.575 Da Detail

LWSLVVLAIE RYVVVCKPMS NFRFGENHAI MGVA

Formula weight

3850.575 Da

Source organism

Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 78.7 %, Completeness (bb): 100.0 % Detail

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Polymer type: polypeptide(L)

	Total	1H
All	78.7 % (163 of 207)	78.7 % (163 of 207)
Backbone	100.0 % (69 of 69)	100.0 % (69 of 69)
Sidechain	68.1 % (94 of 138)	68.1 % (94 of 138)
Aromatic	22.7 % (5 of 22)	22.7 % (5 of 22)
Methyl	68.0 % (17 of 25)	68.0 % (17 of 25)

1. rhodopsin receptor peptide

LWSLVVLAIE RYVVVCKPMS NFRFGENHAI MGVA

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Sample

Temperature 303 (±1) K, pH 4.5 (±0.2)

#	Name	Isotope labeling	Type	Concentration
1	rhodopsin receptor peptide			0.9 mM
2	dodecyphosphocholine			460 mM
3	NaCl			50 mM
4	NaH2PO4			10 mM
5	H2O			90 %
6	D2O			10 %

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Release date

2002-04-08

Citation

NMR structure of the second intracellular loop of the alpha 2A adrenergic receptor: evidence for a novel cytoplasmic helix
Chung, D.A., Zuiderweg, E.R.P., Fowler, C.B., Soyer, O.S., Mosberg, H.I., Neubig, R.R.
Biochemistry (2002), 41, 3596-3604, PubMed 11888275 , DOI: , Abstract

Related entities 1. rhodopsin, : 1 : 38 : 32 : 7 : 94 entities Detail

Interaction partners 1. rhodopsin, : 1 interactors Detail

Experiments performed 3 experiments Detail

Chemical shift validation 3 contents Detail