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NMR Structure of the Second Intracellular Loop of the alpha2A adrenergic receptor: evidence for a Novel Cytoplasmic Helix
Authors
Chung, D.A., Zuiderweg, E.R.P., Fowler, C.B., Soyer, O.S., Mosberg, H.I., Neubig, R.R.
Assembly
rhodopsin
Entity
1. rhodopsin (polymer, Thiol state: all free), 34 monomers, 3850.575 Da Detail

LWSLVVLAIE RYVVVCKPMS NFRFGENHAI MGVA


Formula weight
3850.575 Da
Source organism
Homo sapiens
Exptl. method
NMR
Data set
assigned_chemical_shifts
Chem. Shift Complete
Sequence coverage: 100.0 %, Completeness: 79.7 %, Completeness (bb): 100.0 % Detail

Polymer type: polypeptide(L)

Total1H
All79.7 % (165 of 207)79.7 % (165 of 207)
Backbone100.0 % (67 of 67)100.0 % (67 of 67)
Sidechain70.0 % (98 of 140)70.0 % (98 of 140)
Aromatic22.7 % (5 of 22)22.7 % (5 of 22)
Methyl76.0 % (19 of 25)76.0 % (19 of 25)

1. rhodopsin receptor peptide

LWSLVVLAIE RYVVVCKPMS NFRFGENHAI MGVA

Sample

Temperature 303 (±1) K, pH 4.5 (±0.2)


#NameIsotope labelingTypeConcentration
1rhodopsin receptor peptide0.9 mM
2dodecyphosphocholine460 mM
3NaCl50 mM
4NaH2PO410 mM
5H2O90 %
6D2O10 %

Release date
2002-04-08
Citation
NMR structure of the second intracellular loop of the alpha 2A adrenergic receptor: evidence for a novel cytoplasmic helix
Chung, D.A., Zuiderweg, E.R.P., Fowler, C.B., Soyer, O.S., Mosberg, H.I., Neubig, R.R.
Biochemistry (2002), 41, 3596-3604, PubMed 11888275 , DOI 10.1021/bi015811+ ,
Related entities 1. rhodopsin, : 1 : 36 : 31 : 6 : 58 entities Detail
Interaction partners 1. rhodopsin, : 1 interactors Detail
Experiments performed 3 experiments Detail