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Found 1 Document (4.634 seconds)

BMRB: 5159

1XKE

Sequence-specific resonance assignment of the second Ran-binding domain of human RanBP2

Authors

Doker, R., Geyer, P., Zhao, X., Kremer, W., Villa Braslavsky, C., Kuhlmann, J., Kalbitzer, H.

Assembly

Ran-binding domain 2 of RanBP2

Entity

1. Ran-binding domain 2 of RanBP2 (polymer, Thiol state: all free), 130 monomers, 15115.28 Da Detail

GSGEEDEKVL YSQRVKLFRF DAEVSQWKER GLGNLKILKN EVNGKLRMLM RREQVLKVCA NHWITTTMNL KPLSGSDRAW MWLASDFSDG DAKLEQLAAK FKTPELAEEF KQKFEECQRL LLDIPLQTPK

Formula weight

15115.28 Da

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

Simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.2 %, Completeness: 79.3 %, Completeness (bb): 96.6 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	79.3 % (1269 of 1601)	78.5 % (664 of 846)	77.3 % (474 of 613)	92.3 % (131 of 142)
Backbone	96.6 % (746 of 772)	96.6 % (254 of 263)	96.9 % (371 of 383)	96.0 % (121 of 126)
Sidechain	67.6 % (644 of 952)	70.3 % (410 of 583)	63.5 % (224 of 353)	62.5 % (10 of 16)
Aromatic	0.0 % (0 of 120)	0.0 % (0 of 60)	0.0 % (0 of 56)	0.0 % (0 of 4)
Methyl	72.4 % (97 of 134)	74.6 % (50 of 67)	70.1 % (47 of 67)

1. Ran-binding domain 2 of Ran-binding protein 2

GSGEEDEKVL YSQRVKLFRF DAEVSQWKER GLGNLKILKN EVNGKLRMLM RREQVLKVCA NHWITTTMNL KPLSGSDRAW MWLASDFSDG DAKLEQLAAK FKTPELAEEF KQKFEECQRL LLDIPLQTPK

Show sample condition

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.8 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.7 mM
3	D2O			100 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 5 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.02 ppm, Outliers: 5 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: -0.19 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹³C	DSS	methyl protons	internal	indirect	0.2514495
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	internal	indirect	0.1013291

Referencing offset: 0.18 ppm, Outliers: 1 Detail

Protein Blocks Logo

Calculated from 20 models in PDB: 1XKE, Strand ID: A Detail

Release date

2002-09-29

Citation 1

Sequence-specific resonance assignment of the second Ran-binding domain of human RanBP2
Doker, R., Zhao, X., Kremer, W., Villa Braslavsky, C., Kuhlmann, J., Kalbitzer, H.R.
J. Biomol. NMR (2002), 22, 185-186, PubMed 11883781 , Abstract

Show more 4 citaions

Citation 2

A giant nucleopore protein that binds Ran/TC4
Yokoyama, N., Hayashi, N., Seki, T., Pante, N., Ohba, T., Nishii, K., Kuma, K., Hayashida, T., Miyata, T., Aebi, U.
Nature (1995), 376, 184-188, PubMed 7603572 , DOI 10.1038/376184a0 , Abstract

Citation 3

Nup358, a cytoplasmically exposed nucleoporin with peptide repeats, Ran-GTP binding sites, zinc fingers, a cyclophilin A homologous domain, and a leucine-rich region
Wu, J., Matunis, M.J., Kraemer, D., Blobel, G., Coutavas, E.
J. Biol. Chem. (1995), 270, 14209-14213, PubMed 7775481 , DOI: , Abstract

Citation 4

Structure of a Ran-binding domain complexed with Ran bound to a GTP analogue: implications for nuclear transport
Vetter, I.R., Nowak, C., Nishimoto, T., Kuhlmann, J., Wittinghofer, A.
Nature (1999), 398, 39-46, PubMed 10078529 , DOI 10.1038/17969 , Abstract

Citation 5

Solution structure of the Ran-binding domain 2 of RanBP2 and its interaction with the C terminus of Ran
Geyer, J.P., Doker, R., Kremer, W., Zhao, X., Kuhlmann, J., Kalbitzer, H.R.
J. Mol. Biol. (2005), 348, 711-725, PubMed 15826666 , DOI 10.1016/j.jmb.2005.02.033 , Abstract

Hide non-primary 4 citaions

Related entities 1. Ran-binding domain 2 of RanBP2, : 1 : 204 entities Detail

Interaction partners 1. Ran-binding domain 2 of RanBP2, : 67 interactors Detail

More details for 67 interactors identified by 31 citations

Experiments performed 9 experiments Detail

1 HNCA

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.8 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.7 mM
3	D2O			100 %

Sample #3

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Ran-binding domain 2 of Ran-binding protein 2	[U-15N]		1.1 mM

2 HN(CO)CA

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.8 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.7 mM
3	D2O			100 %

Sample #3

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Ran-binding domain 2 of Ran-binding protein 2	[U-15N]		1.1 mM

3 CBCA(CO)NH

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.8 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.7 mM
3	D2O			100 %

Sample #3

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Ran-binding domain 2 of Ran-binding protein 2	[U-15N]		1.1 mM

4 CBCANH

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.8 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.7 mM
3	D2O			100 %

Sample #3

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Ran-binding domain 2 of Ran-binding protein 2	[U-15N]		1.1 mM

5 HBHA(CO)NH

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.8 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.7 mM
3	D2O			100 %

Sample #3

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Ran-binding domain 2 of Ran-binding protein 2	[U-15N]		1.1 mM

6 HNCO

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.8 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.7 mM
3	D2O			100 %

Sample #3

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Ran-binding domain 2 of Ran-binding protein 2	[U-15N]		1.1 mM

7 1H-15N NOESY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.8 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.7 mM
3	D2O			100 %

Sample #3

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Ran-binding domain 2 of Ran-binding protein 2	[U-15N]		1.1 mM

8 1H-15N TOCSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.8 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.7 mM
3	D2O			100 %

Sample #3

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Ran-binding domain 2 of Ran-binding protein 2	[U-15N]		1.1 mM

9 HCCH-TOCSY

Instrument

#1 Model Bruker DRX (600 MHz)

#2 Model Bruker DRX (800 MHz)

Sample #1

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.8 mM

Sample #2

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
2	Ran-binding domain 2 of Ran-binding protein 2	[U-13C; U-15N]		0.7 mM
3	D2O			100 %

Sample #3

Temperature 298 (±1) K, pH 6.5 (±0.1)

#	Name	Isotope labeling	Type	Concentration
4	Ran-binding domain 2 of Ran-binding protein 2	[U-15N]		1.1 mM

nullKeywords Nup358, Ran-binding domain, RanBP2

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Found 1 Document (4.634 seconds)

BMRB: 5159

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Chemical shift reference

Related entities 1. Ran-binding domain 2 of RanBP2, : 1 : 204 entities Detail

Interaction partners 1. Ran-binding domain 2 of RanBP2, : 67 interactors Detail

Experiments performed 9 experiments Detail

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