BMRB: 5169

NMR solution structure of the human beta2-microglobulin

Authors

Verdone, G., Corazza, A., Viglino, P., Pettirossi, F., Giorgetti, S., Mangione, P., Andreola, A., Stoppini, M., Bellotti, V., Esposito, G.

Assembly

beta2-microglobulin

Entity

1. beta2-microglobulin (polymer, Thiol state: all disulfide bound), 100 monomers, 11862.21 Da Detail

MIQRTPKIQV YSRHPAENGK SNFLNCYVSG FHPSDIEVDL LKNGERIEKV EHSDLSFSKD WSFYLLYYTE FTPTEKDEYA CRVNHVTLSQ PKIVKWDRDM

Formula weight

11862.21 Da

Entity Connection

disulfide 1 Detail

---

ID	Type	Value order	Atom ID 1	Atom ID 2
1	disulfide	sing	1:CYS26:SG	1:CYS81:SG

Source organism

Homo sapiens

Exptl. method

NMR

Refine. method

simulated annealing, restrained molecular dynamics, restrained molecular mechanics

Data set

assigned_chemical_shifts, coupling_constants

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 86.1 %, Completeness (bb): 98.5 % Detail

Download

• NMR-STAR v3.1 (partial)
• BMRB/XML (partial)
• CSV
• TSV

Polymer type: polypeptide(L)

	Total	1H
All	86.1 % (550 of 639)	86.1 % (550 of 639)
Backbone	98.5 % (195 of 198)	98.5 % (195 of 198)
Sidechain	80.5 % (355 of 441)	80.5 % (355 of 441)
Aromatic	66.7 % (46 of 69)	66.7 % (46 of 69)
Methyl	95.6 % (43 of 45)	95.6 % (43 of 45)

1. beta2-microglobulin

MIQRTPKIQV YSRHPAENGK SNFLNCYVSG FHPSDIEVDL LKNGERIEKV EHSDLSFSKD WSFYLLYYTE FTPTEKDEYA CRVNHVTLSQ PKIVKWDRDM

Show sample condition

Sample

Pressure 1 atm, Temperature 310 K, pH 6.4

#	Name	Isotope labeling	Type	Concentration
1	beta2-microglobulin			0.68 mM
2	phosphate buffer			70 mM
3	NaCl			100 mM
4	H2O			90 %
5	D2O			10 %

Hide sample codition

Protein Blocks Logo

Calculated from 20 models in PDB: 1JNJ, Strand ID: A Detail

---

Coupling constant

66 J values in 1 lists
Pressure 1 atm, Temperature 310 K, pH 6.4 Detail

---

Pressure 1 atm, Temperature 310 K, pH 6.4

List #1 coupling_constant_set_1

Release date

2002-05-05

Citation

The solution structure of human beta2-microglobulin reveals the prodromes of its amyloid transition
Verdone, G., Corazza, A., Viglino, P., Pettirossi, F., Giorgetti, S., Mangione, P., Andreola, A., Stoppini, M., Bellotti, V., Esposito, G.
Protein Sci. (2002), 11, 487-499, PubMed 11847272 , DOI 10.1110/ps.29002 , Abstract

Entries sharing articles BMRB: 3 entries Detail

Related entities 1. beta2-microglobulin, : 1 : 99 : 4 : 184 entities Detail

Interaction partners 1. beta2-microglobulin, : 121 interactors Detail

Experiments performed 2 experiments Detail