BMRBj - BMREntryMaster

Found 1 Document (1.351 seconds)

BMRB: 5190

1ZN1

Backbone NMR assignments of Ribosome Recycling Factors from Escherichia coli

Authors

Yoshida, T., Kijima, H., Oka, S., Uchiyama, S., Nakano, H., Ohkubo, T., Kobayashi, Y.

Assembly

ribosome recycling factor

Entity

1. ribosome recycling factor (polymer, Thiol state: all free), 185 monomers, 20638.32 Da Detail

MISDIRKDAE VRMDKCVEAF KTQISKIRTG RASPSLLDGI VVEYYGTPTP LRQLASVTVE DSRTLKINVF DRSMSPAVEK AIMASDLGLN PNSAGSDIRV PLPPLTEERR KDLTKIVRGE AEQARVAVRN VRRDANDKVK ALLKDKEISE DDDRRSQDDV QKLTDAAIKK IEAALADKEA ELMQF

Formula weight

20638.32 Da

Source organism

Escherichia coli

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.5 %, Completeness: 39.7 %, Completeness (bb): 68.2 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	39.7 % (844 of 2124)	23.5 % (262 of 1116)	49.6 % (407 of 820)	93.1 % (175 of 188)
Backbone	68.2 % (746 of 1094)	51.6 % (190 of 368)	69.6 % (382 of 549)	98.3 % (174 of 177)
Sidechain	9.1 % (110 of 1209)	9.5 % (71 of 748)	8.7 % (39 of 450)	0.0 % (0 of 11)
Aromatic	13.0 % (6 of 46)	13.0 % (3 of 23)	13.0 % (3 of 23)
Methyl	10.7 % (24 of 224)	12.5 % (14 of 112)	8.9 % (10 of 112)

1. ribosome recycling factor

Show sample condition

Sample

Temperature 298 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	ribosome recycling factor			0.5 mM

Release date

2002-05-06

Citation

Backbone NMR assignments of ribosome recycling factors (RRFs) from Escherichia coli and Tthermotoga maritima
Yoshida, T., Kijima, H., Oka, S., Uchiyama, S., Nakano, H., Ohkubo, T., Kobayashi, Y.
J. Biomol. NMR (2002), 22, 195-196, PubMed 11883785 , DOI: , Abstract

Related entities 1. ribosome recycling factor, : 1 : 7 : 2 : 151 entities Detail

Interaction partners 1. ribosome recycling factor, : 35 interactors Detail

More details for 35 interactors identified by 3 citations

Chemical shift validation 3 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5190_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	OK

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5190_3.str

#	Content subtype	Saveframe	Status	# of rows	Experiment type	Sequence coverage (%)
1	Assigned chemical shifts	shift_set_1	OK	716		98.9 (chain: 1, length: 185)
1	Chemical shift references	chemical_shift_reference	OK	3		No information

Assigned chemical shifts

Saveframe: shift_set_1
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 185, Sequence coverage: 98.9%
- Total number of assignments
  - ¹³C chemical shifts: 366
  - ¹H chemical shifts: 175
  - ¹⁵N chemical shifts: 175
- Completeness of assignments
  - Entity_assemble_ID: 1
- Redox state of CYS
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	117	0	0.0
¹H chemical shifts	117	0	0.0

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Found 1 Document (1.351 seconds)

BMRB: 5190

Sample

Related entities 1. ribosome recycling factor, : 1 : 7 : 2 : 151 entities Detail

Interaction partners 1. ribosome recycling factor, : 35 interactors Detail

Chemical shift validation 3 contents Detail

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	820	366	44.6
¹H chemical shifts	1116	175	15.7
¹⁵N chemical shifts	205	175	85.4

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹³C chemical shifts	370	366	98.9
¹H chemical shifts	368	175	47.6
¹⁵N chemical shifts	177	175	98.9