1H and 13CA chemical shift assignments of the polypeptide mPrP(143-158)
Polymer type: polypeptide(L)
Total | 1H | 13C | |
---|---|---|---|
All | 65.8 % (129 of 196) | 98.3 % (113 of 115) | 19.8 % (16 of 81) |
Backbone | 58.2 % (46 of 79) | 96.8 % (30 of 31) | 33.3 % (16 of 48) |
Sidechain | 62.4 % (83 of 133) | 98.8 % (83 of 84) | 0.0 % (0 of 49) |
Aromatic | 51.2 % (22 of 43) | 100.0 % (22 of 22) | 0.0 % (0 of 21) |
1. mPrP(143-158)
NDWEDRYYRE NMYRYPTemperature 293 K, pH 4.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | mPrP(143-158) | 1.0 mM | ||
2 | H2O | 90 % | ||
3 | D2O | 10 % |
Polymer type: polypeptide(L)
Total | 1H | 13C | |
---|---|---|---|
All | 46.2 % (181 of 392) | 70.4 % (162 of 230) | 11.7 % (19 of 162) |
Backbone | 39.9 % (63 of 158) | 71.0 % (44 of 62) | 19.8 % (19 of 96) |
Sidechain | 44.7 % (119 of 266) | 70.2 % (118 of 168) | 1.0 % (1 of 98) |
Aromatic | 34.9 % (30 of 86) | 68.2 % (30 of 44) | 0.0 % (0 of 42) |
1. mPrP(143-158)
NDWEDRYYRE NMYRYPTemperature 293 K, pH 4.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | mPrP(143-158) | 1.0 mM | ||
2 | H2O | 90 % | ||
3 | D2O | 10 % |
Polymer type: polypeptide(L)
Total | 1H | 13C | |
---|---|---|---|
All | 53.4 % (314 of 588) | 79.7 % (275 of 345) | 16.0 % (39 of 243) |
Backbone | 46.8 % (111 of 237) | 79.6 % (74 of 93) | 25.7 % (37 of 144) |
Sidechain | 51.4 % (205 of 399) | 79.8 % (201 of 252) | 2.7 % (4 of 147) |
Aromatic | 40.3 % (52 of 129) | 78.8 % (52 of 66) | 0.0 % (0 of 63) |
1. mPrP(143-158)
NDWEDRYYRE NMYRYPTemperature 293 K, pH 4.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | mPrP(143-158) | 1.0 mM | ||
5 | H2O | 90 % | ||
6 | TFE | [U-2H] | 10 % |
Polymer type: polypeptide(L)
Total | 1H | 13C | |
---|---|---|---|
All | 46.9 % (368 of 784) | 70.0 % (322 of 460) | 14.2 % (46 of 324) |
Backbone | 40.5 % (128 of 316) | 69.4 % (86 of 124) | 21.9 % (42 of 192) |
Sidechain | 45.9 % (244 of 532) | 70.2 % (236 of 336) | 4.1 % (8 of 196) |
Aromatic | 37.2 % (64 of 172) | 72.7 % (64 of 88) | 0.0 % (0 of 84) |
1. mPrP(143-158)
NDWEDRYYRE NMYRYPTemperature 293 K, pH 4.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
4 | mPrP(143-158) | 1.0 mM | ||
5 | H2O | 90 % | ||
6 | TFE | [U-2H] | 10 % |
Polymer type: polypeptide(L)
Total | 1H | 13C | |
---|---|---|---|
All | 51.1 % (501 of 980) | 75.7 % (435 of 575) | 16.3 % (66 of 405) |
Backbone | 44.8 % (177 of 395) | 74.8 % (116 of 155) | 25.4 % (61 of 240) |
Sidechain | 49.6 % (330 of 665) | 76.0 % (319 of 420) | 4.5 % (11 of 245) |
Aromatic | 40.0 % (86 of 215) | 78.2 % (86 of 110) | 0.0 % (0 of 105) |
1. mPrP(143-158)
NDWEDRYYRE NMYRYPTemperature 293 K, pH 4.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | mPrP(143-158) | 1.0 mM | ||
8 | H2O | 75 % | ||
9 | TFE | [U-2H] | 25 % |
Polymer type: polypeptide(L)
Total | 1H | 13C | |
---|---|---|---|
All | 46.5 % (547 of 1176) | 69.4 % (479 of 690) | 14.0 % (68 of 486) |
Backbone | 40.1 % (190 of 474) | 68.3 % (127 of 186) | 21.9 % (63 of 288) |
Sidechain | 45.6 % (364 of 798) | 69.8 % (352 of 504) | 4.1 % (12 of 294) |
Aromatic | 36.4 % (94 of 258) | 71.2 % (94 of 132) | 0.0 % (0 of 126) |
1. mPrP(143-158)
NDWEDRYYRE NMYRYPTemperature 293 K, pH 4.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
7 | mPrP(143-158) | 1.0 mM | ||
8 | H2O | 75 % | ||
9 | TFE | [U-2H] | 25 % |
Polymer type: polypeptide(L)
Total | 1H | 13C | |
---|---|---|---|
All | 49.3 % (676 of 1372) | 73.5 % (592 of 805) | 14.8 % (84 of 567) |
Backbone | 42.7 % (236 of 553) | 72.4 % (157 of 217) | 23.5 % (79 of 336) |
Sidechain | 48.0 % (447 of 931) | 74.0 % (435 of 588) | 3.5 % (12 of 343) |
Aromatic | 38.5 % (116 of 301) | 75.3 % (116 of 154) | 0.0 % (0 of 147) |
1. mPrP(143-158)
NDWEDRYYRE NMYRYPTemperature 293 K, pH 4.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | mPrP(143-158) | 1.0 mM | ||
11 | H2O | 50 % | ||
12 | TFE | [U-2H] | 50 % |
Polymer type: polypeptide(L)
Total | 1H | 13C | |
---|---|---|---|
All | 46.4 % (728 of 1568) | 69.2 % (637 of 920) | 14.0 % (91 of 648) |
Backbone | 40.0 % (253 of 632) | 67.7 % (168 of 248) | 22.1 % (85 of 384) |
Sidechain | 45.5 % (484 of 1064) | 69.8 % (469 of 672) | 3.8 % (15 of 392) |
Aromatic | 36.3 % (125 of 344) | 70.5 % (124 of 176) | 0.6 % (1 of 168) |
1. mPrP(143-158)
NDWEDRYYRE NMYRYPTemperature 293 K, pH 4.5
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
10 | mPrP(143-158) | 1.0 mM | ||
11 | H2O | 50 % | ||
12 | TFE | [U-2H] | 50 % |