BMRBj - BMREntryMaster

Found 1 Document (1.297 seconds)

BMRB: 5208

3UXN

1H, 13C and 15N resonance assignments for the perdeuterated 22 kD palm-thumb domain of DNA polymerase B

Authors

Gryk, M.R., Maciejewski, M.W., Robertson, A., Mullen, M.A., Wilson, S.H., Mullen, G.P.

Assembly

Palm-Thumb Domain of DNA Polymerase B

Entity

1. Palm-Thumb Domain of DNA Polymerase B (polymer, Thiol state: all free), 190 monomers, 22021.77 Da Detail

MGKRIPREEM LQMQDIVLNE VKKLDPEYIA TVCGSFRRGA ESSGDMDVLL THPNFTSESS KQPKLLHRVV EQLQKVRFIT DTLSKGETKF MGVCQLPSEN DENEYPHRRI DIRLIPKDQY YCGVLYFTGS DIFNKNMRAH ALEKGFTINE YTIRPLGVTG VAGEPLPVDS EQDIFDYIQW RYREPKDRSE

Formula weight

22021.77 Da

Source organism

Rattus norvegicus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 97.9 %, Completeness: 52.0 %, Completeness (bb): 81.4 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	52.0 % (1183 of 2277)	30.2 % (362 of 1199)	73.7 % (650 of 882)	87.2 % (171 of 196)
Backbone	81.4 % (910 of 1118)	53.5 % (204 of 381)	96.1 % (536 of 558)	95.0 % (170 of 179)
Sidechain	33.1 % (443 of 1337)	19.3 % (158 of 818)	56.6 % (284 of 502)	5.9 % (1 of 17)
Aromatic	7.6 % (13 of 172)	5.8 % (5 of 86)	9.4 % (8 of 85)	0.0 % (0 of 1)
Methyl	73.7 % (137 of 186)	72.0 % (67 of 93)	75.3 % (70 of 93)

1. DNA Polymerase B

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.8 (±0.2), Details AEBSF is 4-(2-aminoethyl)-benzenesulfonyl fluoride.

#	Name	Isotope labeling	Concentration
1	DNA Polymerase B	U-98% 13C; U-99% 15N; U-97% 2H; except V g-methyls, L d-methyls, I d-methyl, and labile protons.	1.0 mM
2	phosphate		46 mM
3	sodium chloride		135 mM
4	sodium azide		0.002 %
5	AEBSF		100 uM
6	dithiothreitol (DTT)		1 mM
7	H2O		90 %
8	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.41 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.41 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.2 ppm, Outliers: 3 Detail

Protein Blocks Logo

Calculated from 1 models in PDB: 1BPD, Strand ID: A Detail

Release date

2002-05-06

Citation

1H, 13C and 15N resonance assignments for the perdeuterated 22 kD palm-thumb domain of DNA polymerase beta
Gryk, M.R., Maciejewski, M.W., Robertson, A., Mullen, M.A., Wilson, S.H., Mullen, G.P.
J. Biomol. NMR (2002), 22, 197-198, PubMed 11883786 , Abstract

Related entities 1. Palm-Thumb Domain of DNA Polymerase B, : 1 : 26 : 130 entities Detail

Interaction partners 1. Palm-Thumb Domain of DNA Polymerase B, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 9 experiments Detail

1 2D TROSY 1H-15N HSQC