BMRBj - BMREntryMaster

Found 1 Document (1.669 seconds)

BMRB: 5209

1BNP

1H, 13C and 15N resonance assignments for the perdeuterated 22 kD palm-thumb domain of DNA polymerase B in complex with the XRCC1 N-terminal domain

Authors

Gryk, M.R., Marintchev, A., Maciejewski, M.W., Robertson, A., Mullen, M.A., Wilson, S.H., Mullen, G.P.

Assembly

Palm-Thumb Domain of DNA Polymerase B in complex with XRCC1-NTD

Entity

1. DNA Polymerase B (polymer, Thiol state: all free), 190 monomers, 22021.77 Da Detail

MGKRIPREEM LQMQDIVLNE VKKLDPEYIA TVCGSFRRGA ESSGDMDVLL THPNFTSESS KQPKLLHRVV EQLQKVRFIT DTLSKGETKF MGVCQLPSEN DENEYPHRRI DIRLIPKDQY YCGVLYFTGS DIFNKNMRAH ALEKGFTINE YTIRPLGVTG VAGEPLPVDS EQDIFDYIQW RYREPKDRSE

2. XRCC1 N-terminal Domain (polymer, Thiol state: all free), 157 monomers, 17358.29 Da Detail

MPEIRLRHVV SCSSQDSTHC AENLLKADTY RKWRAAKAGE KTISVVLQLE KEEQIHSVDI GNDGSAFVEV LVGSSAGGAG EQDYEVLLVT SSFMSPSESR SGSNPNRVRM FGPDKLVRAA AEKRWDRVKI VCSQPYSKDS PFGLSFVRFH SPPDKDE

Total weight

39380.06 Da

Max. entity weight

22021.77 Da

Source organism

Rattus norvegicus , Homo sapiens

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 53.6 %, Completeness: 24.9 %, Completeness (bb): 38.7 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	24.9 % (1011 of 4057)	15.2 % (324 of 2128)	33.1 % (520 of 1573)	46.9 % (167 of 356)
Backbone	38.7 % (791 of 2044)	27.5 % (192 of 697)	42.6 % (434 of 1019)	50.3 % (165 of 328)
Sidechain	14.7 % (344 of 2338)	9.2 % (132 of 1431)	23.9 % (210 of 879)	7.1 % (2 of 28)
Aromatic	7.8 % (23 of 296)	13.5 % (20 of 148)	1.4 % (2 of 145)	33.3 % (1 of 3)
Methyl	34.2 % (115 of 336)	34.5 % (58 of 168)	33.9 % (57 of 168)

1. DNA Polymerase B

2. XRCC1 N-terminal Domain

MPEIRLRHVV SCSSQDSTHC AENLLKADTY RKWRAAKAGE KTISVVLQLE KEEQIHSVDI GNDGSAFVEV LVGSSAGGAG EQDYEVLLVT SSFMSPSESR SGSNPNRVRM FGPDKLVRAA AEKRWDRVKI VCSQPYSKDS PFGLSFVRFH SPPDKDE

Show sample condition

Sample

Temperature 298 (±1) K, pH 6.8 (±0.2), Details AEBSF is 4-(2-aminoethyl)-benzenesulfonyl fluoride.

#	Name	Isotope labeling	Concentration
1	DNA Polymerase B	[U-98% 13C; U-99% 15N; U-97% 2H; except V g-methyls, L d-methyls, I d-methyl, and labile protons.	700 uM
2	XRCC1 N-terminal Domain		1.1 mM
3	phosphate		46 mM
4	sodium chloride		135 mM
5	sodium azide		0.002 %
6	AEBSF		100 uM
7	dithiothreitol (DTT)		5 mM
8	H2O		90 %
9	D2O		10 %

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.53 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.53 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.42 ppm, Outliers: 2 Detail

Release date

2005-06-01

Citation

Mapping of the interaction interface of DNA polymerase beta with XRCC1
Gryk, M.R., Marintchev, A., Maciejewski, M.W., Robertson, A., Wilson, S.H., Mullen, G.P.
Structure (2002), 10, 1709-1720, PubMed 12467578 , DOI: , Abstract

Related entities 1. DNA Polymerase B, : 2 : 55 entities Detail

Related entities 2. XRCC1 N-terminal Domain, : 5 : 1 : 7 entities Detail

Interaction partners 1. DNA Polymerase B, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 7 experiments Detail

1 2D TROSY 1H-15N HSQC