BMRBj - BMREntryMaster

Found 1 Document (1.996 seconds)

BMRB: 5213

1K0V

Copper Trafficking: The Solution Structure of Bacillus subtilis CopZ

Authors

Banci, L., Bertini, I., Del Conte, R., Markey, J., Ruiz-Duenas, J.F.

Assembly

CopZ from Bacillus subtilis

Entity

1. CopZ from Bacillus subtilis (polymer, Thiol state: all other bound), 73 monomers, 7793.614 Da Detail

MEQKTLQVEG MSCQHCVKAV ETSVGELDGV SAVHVNLEAG KVDVSFDADK VSVKDIADAI EDQGYDVAKI EGR

2. COPPER (I) ION (non-polymer), 63.546 Da

Total weight

7857.1597 Da

Max. entity weight

7793.614 Da

Entity Connection

na 2 Detail

ID	Type	Value order	Atom ID 1	Atom ID 2
1	na	sing	1:CYS13:SG	2:CU11:CU
2	na	sing	1:CYS16:SG	2:CU11:CU

Source organism

Bacillus subtilis

Exptl. method

NMR

Refine. method

simulated annealing

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 100.0 %, Completeness: 92.7 %, Completeness (bb): 99.1 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹⁵N
All	92.7 % (444 of 479)	92.5 % (371 of 401)	93.6 % (73 of 78)
Backbone	99.1 % (223 of 225)	99.3 % (151 of 152)	98.6 % (72 of 73)
Sidechain	87.0 % (221 of 254)	88.4 % (220 of 249)	20.0 % (1 of 5)
Aromatic	61.5 % (8 of 13)	61.5 % (8 of 13)
Methyl	93.3 % (42 of 45)	93.3 % (42 of 45)

1. CopZ from Bacillus subtilis

MEQKTLQVEG MSCQHCVKAV ETSVGELDGV SAVHVNLEAG KVDVSFDADK VSVKDIADAI EDQGYDVAKI EGR

Show sample condition

Sample

Pressure 1 atm, Temperature 300 (±0.5) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	CopZ from Bacillus subtilis	[U-99% 15N]	2 mM
2	COPPER (I) ION		2 mM
3	phosphate buffer		100 mM
4	DTT		trace mM
5	D2O		10 %
6	H2O		90 %

Protein Blocks Logo

Calculated from 30 models in PDB: 1K0V, Strand ID: A Detail

Release date

2001-11-19

Citation

Copper trafficking: the solution structure of Bacillus subtilis CopZ
Banci, L., Bertini, I., Del Conte, R., Markey, J., Ruiz-Duenas, F.J.
Biochemistry (2001), 40, 15660-15668, PubMed 11747441 , DOI 10.2210/pdb1k0v/pdb , Abstract

Related entities 1. CopZ from Bacillus subtilis, : 1 : 1 : 3 : 355 entities Detail

Interaction partners 1. CopZ from Bacillus subtilis, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 6 experiments Detail

1 3D TOSCY-HMQC

Instrument

Model Bruker AVANCE (700 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.5) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	CopZ from Bacillus subtilis	[U-99% 15N]	2 mM
2	COPPER (I) ION		2 mM
3	phosphate buffer		100 mM
4	DTT		trace mM
5	D2O		10 %
6	H2O		90 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.5) K, pH 7.0 (±0.01)

#	Name	Concentration
7	CopZ from Bacillus subtilis	2 mM
8	COPPER (I) ION	2 mM
9	phosphate buffer	100 mM
10	DTT	trace mM
11	D2O	10 %
12	H2O	90 %

2 3D NOESY-HSQC

Instrument

Model Bruker AVANCE (700 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.5) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	CopZ from Bacillus subtilis	[U-99% 15N]	2 mM
2	COPPER (I) ION		2 mM
3	phosphate buffer		100 mM
4	DTT		trace mM
5	D2O		10 %
6	H2O		90 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.5) K, pH 7.0 (±0.01)

#	Name	Concentration
7	CopZ from Bacillus subtilis	2 mM
8	COPPER (I) ION	2 mM
9	phosphate buffer	100 mM
10	DTT	trace mM
11	D2O	10 %
12	H2O	90 %

3 3D HNHA

Instrument

Model Bruker AVANCE (700 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.5) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	CopZ from Bacillus subtilis	[U-99% 15N]	2 mM
2	COPPER (I) ION		2 mM
3	phosphate buffer		100 mM
4	DTT		trace mM
5	D2O		10 %
6	H2O		90 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.5) K, pH 7.0 (±0.01)

#	Name	Concentration
7	CopZ from Bacillus subtilis	2 mM
8	COPPER (I) ION	2 mM
9	phosphate buffer	100 mM
10	DTT	trace mM
11	D2O	10 %
12	H2O	90 %

4 2D TOSCY

Instrument

Model Bruker AVANCE (700 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.5) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	CopZ from Bacillus subtilis	[U-99% 15N]	2 mM
2	COPPER (I) ION		2 mM
3	phosphate buffer		100 mM
4	DTT		trace mM
5	D2O		10 %
6	H2O		90 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.5) K, pH 7.0 (±0.01)

#	Name	Concentration
7	CopZ from Bacillus subtilis	2 mM
8	COPPER (I) ION	2 mM
9	phosphate buffer	100 mM
10	DTT	trace mM
11	D2O	10 %
12	H2O	90 %

5 2D NOESY

Instrument

Model Bruker AVANCE (700 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.5) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	CopZ from Bacillus subtilis	[U-99% 15N]	2 mM
2	COPPER (I) ION		2 mM
3	phosphate buffer		100 mM
4	DTT		trace mM
5	D2O		10 %
6	H2O		90 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.5) K, pH 7.0 (±0.01)

#	Name	Concentration
7	CopZ from Bacillus subtilis	2 mM
8	COPPER (I) ION	2 mM
9	phosphate buffer	100 mM
10	DTT	trace mM
11	D2O	10 %
12	H2O	90 %

6 2D HSQC

Instrument

Model Bruker AVANCE (700 MHz)

Sample #1

Pressure 1 atm, Temperature 300 (±0.5) K, pH 7.0 (±0.01)

#	Name	Isotope labeling	Concentration
1	CopZ from Bacillus subtilis	[U-99% 15N]	2 mM
2	COPPER (I) ION		2 mM
3	phosphate buffer		100 mM
4	DTT		trace mM
5	D2O		10 %
6	H2O		90 %

Sample #2

Pressure 1 atm, Temperature 300 (±0.5) K, pH 7.0 (±0.01)

#	Name	Concentration
7	CopZ from Bacillus subtilis	2 mM
8	COPPER (I) ION	2 mM
9	phosphate buffer	100 mM
10	DTT	trace mM
11	D2O	10 %
12	H2O	90 %

nullKeywords beta-alpha-beta-beta-alpha-beta

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Found 1 Document (1.996 seconds)

BMRB: 5213

Sample

Related entities 1. CopZ from Bacillus subtilis, : 1 : 1 : 3 : 355 entities Detail

Interaction partners 1. CopZ from Bacillus subtilis, : 1 interactors Detail

Experiments performed 6 experiments Detail

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