BMRBj - BMREntryMaster

Found 1 Document (1.449 seconds)

BMRB: 5226

1H0X

Letter to the Editor: 1H, 15N and 13C resonance assignments and secondary structure determination of the Ssh10b from Hyperthermphilic Archaeon Sulfolobus shibatae

Authors

Tong, Y., Cui, Q., Feng, Y., Huang, L., Wang, J.

Assembly

Ssh10b dimer

Entity

1. Ssh10b dimer (polymer, Thiol state: not present), 97 monomers, 10585.31 × 2 Da Detail

MSSGTPTPSN VVLIGKKPVM NYVLAALTLL NQGVSEIVIK ARGRAISKAV DTVEIVRNRF LPDKIEIKEI RVGSQVVTSQ DGRQSRVSTI EIAIRKK

Total weight

21170.62 Da

Max. entity weight

10585.31 Da

Source organism

Saccharolobus shibatae

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete

Sequence coverage: 99.0 %, Completeness: 95.6 %, Completeness (bb): 97.9 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	95.6 % (1078 of 1128)	94.7 % (558 of 589)	96.3 % (422 of 438)	97.0 % (98 of 101)
Backbone	97.9 % (562 of 574)	97.4 % (191 of 196)	98.6 % (281 of 285)	96.8 % (90 of 93)
Sidechain	94.0 % (606 of 645)	93.4 % (367 of 393)	94.7 % (231 of 244)	100.0 % (8 of 8)
Aromatic	22.2 % (4 of 18)	22.2 % (2 of 9)	22.2 % (2 of 9)
Methyl	100.0 % (144 of 144)	100.0 % (72 of 72)	100.0 % (72 of 72)

1. Ssh10b

MSSGTPTPSN VVLIGKKPVM NYVLAALTLL NQGVSEIVIK ARGRAISKAV DTVEIVRNRF LPDKIEIKEI RVGSQVVTSQ DGRQSRVSTI EIAIRKK

Show sample condition

Sample

Temperature 310 (±0.1) K, pH 5.0 (±0.1)

#	Name	Isotope labeling	Type	Concentration
1	Ssh10b	[U-95% 13C; U-98% 15N]		1.5 mM

LACS Plot; CA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.26 ppm, Outliers: 1 Detail

LACS Plot; CB

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.26 ppm, Outliers: 1 Detail

LACS Plot; HA

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: -0.17 ppm, Outliers: 1 Detail

LACS Plot; CO

Atom type	Mol. common name	Atom group	Ref. method	Ref. type	Shift ratio
¹H	DSS	methyl protons	internal	direct	1.0
¹⁵N	DSS	methyl protons	null	indirect	0.1013291
¹³C	DSS	methyl protons	null	indirect	0.2514495

Referencing offset: 0.36 ppm, Outliers: 1 Detail

Release date

2002-12-26

Citation 1

1H, 15N, and 13C resonance assignments and secondary structure of the Ssh10b from hyperthermophilic archaeon Sulfolobus shibatae
Tong, Y., Cui, Q., Feng, Y., Huang, L., Wang, J.
J. Biomol. NMR (2002), 22, 385-386, PubMed 12018492 , Abstract

Show more 1 citaion

Citation 2

An abundant DNA binding protein from the hyperthermophilic archaeon Sulfolobus shibatae affects DNA supercoiling in a temperature-dependent fashion
Xue, H., Guo, R., Wen, Y., Liu, D., Huang, L.
J. Bacteriol. (2000), 182, 3929-3933, PubMed 10869069 , DOI: , Abstract

Hide non-primary 1 citaion

Entries sharing articles BMRB: 1, Swiss-Prot: 1 entries Detail

  BMRB: 6019 released on 2004-06-24
    Title Solution structure of Archaeon DNA-binding protein ssh10b P62A mutant
  Swiss-Prot: P60848 released on 2004-04-13
    Title ALBA1_SULSH Entity DNA/RNA-binding protein Alba 1

Related entities 1. Ssh10b dimer, : 1 : 4 : 1 : 43 entities Detail

Experiments performed 13 experiments Detail

1 HNCA