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BMRB: 5228

Letter to the Editor: Assignment of a 15 kDa protein complex formed between the p160 coactivator ACTR and the CREB binding protein

Authors

Demarest, S.J., Chung, J.H., Dyson, H.J., Wright, P.E.

Assembly

ACTR in complex with the CREB binding protein, CBP

Entity

1. ACTR (polymer, Thiol state: not present), 71 monomers, 7701.357 Da Detail

GTQNRPLLRN SLDDLVGPPS NLEGQSDERA LLDQLHTLLS NTDATGLEEI DRALGIPELV NQGQALEPKQ D

2. CREB binding protein (polymer, Thiol state: not present), 59 monomers, 6561.503 Da Detail

PNRSISPSAL QDLLRTLKSP SSPQQQQQVL NILKSNPQLM AAFIKQRTAK YVANQPGMQ

Total weight

14262.859 Da

Max. entity weight

7701.357 Da

Source organism

Homo sapiens , Mus musculus

Exptl. method

NMR

Data set

assigned_chemical_shifts

Chem. Shift Complete1

Sequence coverage: 56.9 %, Completeness: 45.7 %, Completeness (bb): 55.3 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	45.7 % (686 of 1502)	42.1 % (335 of 795)	49.6 % (279 of 563)	50.0 % (72 of 144)
Backbone	55.3 % (419 of 758)	55.1 % (141 of 256)	54.8 % (210 of 383)	57.1 % (68 of 119)
Sidechain	38.6 % (335 of 867)	36.2 % (195 of 539)	44.9 % (136 of 303)	16.0 % (4 of 25)
Aromatic	0.0 % (0 of 22)	0.0 % (0 of 11)	0.0 % (0 of 11)
Methyl	42.7 % (64 of 150)	42.7 % (32 of 75)	42.7 % (32 of 75)

1. ACTR

GTQNRPLLRN SLDDLVGPPS NLEGQSDERA LLDQLHTLLS NTDATGLEEI DRALGIPELV NQGQALEPKQ D

2. CREB binding protein

PNRSISPSAL QDLLRTLKSP SSPQQQQQVL NILKSNPQLM AAFIKQRTAK YVANQPGMQ

Show sample condition

Sample #1

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	ACTR	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
2	CREB binding protein		1.0 ~ 5.0 mM
3	TrisHCl		10 mM
4	NaCl		50 mM

Sample #2

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	ACTR	[U-90% 15N]	1.0 ~ 5.0 mM
6	CREB binding protein		1.0 ~ 5.0 mM
7	TrisHCl		10 mM
8	NaCl		50 mM

Chem. Shift Complete2

Sequence coverage: 54.6 %, Completeness: 44.9 %, Completeness (bb): 50.8 % Detail

Polymer type: polypeptide(L)

	Total	¹H	¹³C	¹⁵N
All	44.9 % (1349 of 3004)	43.1 % (685 of 1590)	47.2 % (531 of 1126)	46.2 % (133 of 288)
Backbone	50.8 % (770 of 1516)	51.4 % (263 of 512)	50.1 % (384 of 766)	51.7 % (123 of 238)
Sidechain	40.7 % (706 of 1734)	39.2 % (423 of 1078)	45.0 % (273 of 606)	20.0 % (10 of 50)
Aromatic	40.9 % (18 of 44)	40.9 % (9 of 22)	40.9 % (9 of 22)
Methyl	46.0 % (138 of 300)	47.3 % (71 of 150)	44.7 % (67 of 150)

1. ACTR

GTQNRPLLRN SLDDLVGPPS NLEGQSDERA LLDQLHTLLS NTDATGLEEI DRALGIPELV NQGQALEPKQ D

2. CREB binding protein

PNRSISPSAL QDLLRTLKSP SSPQQQQQVL NILKSNPQLM AAFIKQRTAK YVANQPGMQ

Show sample condition

Sample #1

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
9	ACTR		1.0 ~ 5.0 mM
10	CREB binding protein	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
11	TrisHCl		10 mM
12	NaCl		50 mM

Sample #2

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	ACTR		1.0 ~ 5.0 mM
14	CREB binding protein	[U-90% 15N]	1.0 ~ 5.0 mM
15	TrisHCl		10 mM
16	NaCl		50 mM

Release date

2002-08-21

Citation

Assignment of a 15 kDa protein complex formed between the p160 coactivator ACTR and CREB binding protein
Demarest, S.J., Chung, J.H., Dyson, H.J., Wright, P.E.
J. Biomol. NMR (2002), 22, 377-378, PubMed 12018488 , Abstract

Related entities 1. ACTR, : 2 : 25 entities Detail

Related entities 2. CREB binding protein, : 6 : 16 entities Detail

Interaction partners 1. ACTR, : 54 : 1 interactors Detail

More details for 55 interactors identified by 26 citations

Interaction partners 2. CREB binding protein, : 1 interactors Detail

More details for 1 interactors identified by 1 citations

Experiments performed 17 experiments Detail

1 3D 1H-15N HSQC NOESY

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DMX (600 MHz)

#4 Model Bruker AMX (600 MHz)

#5 Model Bruker DMX (750 MHz)

#6 Model Bruker DRX (800 MHz)

Sample #1

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	ACTR	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
2	CREB binding protein		1.0 ~ 5.0 mM
3	TrisHCl		10 mM
4	NaCl		50 mM

Sample #2

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	ACTR	[U-90% 15N]	1.0 ~ 5.0 mM
6	CREB binding protein		1.0 ~ 5.0 mM
7	TrisHCl		10 mM
8	NaCl		50 mM

Sample #3

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
9	ACTR		1.0 ~ 5.0 mM
10	CREB binding protein	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
11	TrisHCl		10 mM
12	NaCl		50 mM

Sample #4

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	ACTR		1.0 ~ 5.0 mM
14	CREB binding protein	[U-90% 15N]	1.0 ~ 5.0 mM
15	TrisHCl		10 mM
16	NaCl		50 mM

2 3D 1H-13C HSQC NOESY

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DMX (600 MHz)

#4 Model Bruker AMX (600 MHz)

#5 Model Bruker DMX (750 MHz)

#6 Model Bruker DRX (800 MHz)

Sample #1

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	ACTR	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
2	CREB binding protein		1.0 ~ 5.0 mM
3	TrisHCl		10 mM
4	NaCl		50 mM

Sample #2

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	ACTR	[U-90% 15N]	1.0 ~ 5.0 mM
6	CREB binding protein		1.0 ~ 5.0 mM
7	TrisHCl		10 mM
8	NaCl		50 mM

Sample #3

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
9	ACTR		1.0 ~ 5.0 mM
10	CREB binding protein	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
11	TrisHCl		10 mM
12	NaCl		50 mM

Sample #4

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	ACTR		1.0 ~ 5.0 mM
14	CREB binding protein	[U-90% 15N]	1.0 ~ 5.0 mM
15	TrisHCl		10 mM
16	NaCl		50 mM

3 3D 12C-filtered/1H-13C-edited NOESY

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DMX (600 MHz)

#4 Model Bruker AMX (600 MHz)

#5 Model Bruker DMX (750 MHz)

#6 Model Bruker DRX (800 MHz)

Sample #1

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	ACTR	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
2	CREB binding protein		1.0 ~ 5.0 mM
3	TrisHCl		10 mM
4	NaCl		50 mM

Sample #2

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	ACTR	[U-90% 15N]	1.0 ~ 5.0 mM
6	CREB binding protein		1.0 ~ 5.0 mM
7	TrisHCl		10 mM
8	NaCl		50 mM

Sample #3

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
9	ACTR		1.0 ~ 5.0 mM
10	CREB binding protein	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
11	TrisHCl		10 mM
12	NaCl		50 mM

Sample #4

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	ACTR		1.0 ~ 5.0 mM
14	CREB binding protein	[U-90% 15N]	1.0 ~ 5.0 mM
15	TrisHCl		10 mM
16	NaCl		50 mM

4 HNCA

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DMX (600 MHz)

#4 Model Bruker AMX (600 MHz)

#5 Model Bruker DMX (750 MHz)

#6 Model Bruker DRX (800 MHz)

Sample #1

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	ACTR	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
2	CREB binding protein		1.0 ~ 5.0 mM
3	TrisHCl		10 mM
4	NaCl		50 mM

Sample #2

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	ACTR	[U-90% 15N]	1.0 ~ 5.0 mM
6	CREB binding protein		1.0 ~ 5.0 mM
7	TrisHCl		10 mM
8	NaCl		50 mM

Sample #3

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
9	ACTR		1.0 ~ 5.0 mM
10	CREB binding protein	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
11	TrisHCl		10 mM
12	NaCl		50 mM

Sample #4

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	ACTR		1.0 ~ 5.0 mM
14	CREB binding protein	[U-90% 15N]	1.0 ~ 5.0 mM
15	TrisHCl		10 mM
16	NaCl		50 mM

5 HNCO

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DMX (600 MHz)

#4 Model Bruker AMX (600 MHz)

#5 Model Bruker DMX (750 MHz)

#6 Model Bruker DRX (800 MHz)

Sample #1

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	ACTR	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
2	CREB binding protein		1.0 ~ 5.0 mM
3	TrisHCl		10 mM
4	NaCl		50 mM

Sample #2

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	ACTR	[U-90% 15N]	1.0 ~ 5.0 mM
6	CREB binding protein		1.0 ~ 5.0 mM
7	TrisHCl		10 mM
8	NaCl		50 mM

Sample #3

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
9	ACTR		1.0 ~ 5.0 mM
10	CREB binding protein	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
11	TrisHCl		10 mM
12	NaCl		50 mM

Sample #4

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	ACTR		1.0 ~ 5.0 mM
14	CREB binding protein	[U-90% 15N]	1.0 ~ 5.0 mM
15	TrisHCl		10 mM
16	NaCl		50 mM

6 CBCACONH

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DMX (600 MHz)

#4 Model Bruker AMX (600 MHz)

#5 Model Bruker DMX (750 MHz)

#6 Model Bruker DRX (800 MHz)

Sample #1

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	ACTR	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
2	CREB binding protein		1.0 ~ 5.0 mM
3	TrisHCl		10 mM
4	NaCl		50 mM

Sample #2

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	ACTR	[U-90% 15N]	1.0 ~ 5.0 mM
6	CREB binding protein		1.0 ~ 5.0 mM
7	TrisHCl		10 mM
8	NaCl		50 mM

Sample #3

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
9	ACTR		1.0 ~ 5.0 mM
10	CREB binding protein	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
11	TrisHCl		10 mM
12	NaCl		50 mM

Sample #4

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	ACTR		1.0 ~ 5.0 mM
14	CREB binding protein	[U-90% 15N]	1.0 ~ 5.0 mM
15	TrisHCl		10 mM
16	NaCl		50 mM

7 HBHACONH

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DMX (600 MHz)

#4 Model Bruker AMX (600 MHz)

#5 Model Bruker DMX (750 MHz)

#6 Model Bruker DRX (800 MHz)

Sample #1

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	ACTR	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
2	CREB binding protein		1.0 ~ 5.0 mM
3	TrisHCl		10 mM
4	NaCl		50 mM

Sample #2

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	ACTR	[U-90% 15N]	1.0 ~ 5.0 mM
6	CREB binding protein		1.0 ~ 5.0 mM
7	TrisHCl		10 mM
8	NaCl		50 mM

Sample #3

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
9	ACTR		1.0 ~ 5.0 mM
10	CREB binding protein	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
11	TrisHCl		10 mM
12	NaCl		50 mM

Sample #4

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	ACTR		1.0 ~ 5.0 mM
14	CREB binding protein	[U-90% 15N]	1.0 ~ 5.0 mM
15	TrisHCl		10 mM
16	NaCl		50 mM

8 HNCACB

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DMX (600 MHz)

#4 Model Bruker AMX (600 MHz)

#5 Model Bruker DMX (750 MHz)

#6 Model Bruker DRX (800 MHz)

Sample #1

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	ACTR	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
2	CREB binding protein		1.0 ~ 5.0 mM
3	TrisHCl		10 mM
4	NaCl		50 mM

Sample #2

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	ACTR	[U-90% 15N]	1.0 ~ 5.0 mM
6	CREB binding protein		1.0 ~ 5.0 mM
7	TrisHCl		10 mM
8	NaCl		50 mM

Sample #3

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
9	ACTR		1.0 ~ 5.0 mM
10	CREB binding protein	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
11	TrisHCl		10 mM
12	NaCl		50 mM

Sample #4

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	ACTR		1.0 ~ 5.0 mM
14	CREB binding protein	[U-90% 15N]	1.0 ~ 5.0 mM
15	TrisHCl		10 mM
16	NaCl		50 mM

9 2D CGCO

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DMX (600 MHz)

#4 Model Bruker AMX (600 MHz)

#5 Model Bruker DMX (750 MHz)

#6 Model Bruker DRX (800 MHz)

Sample #1

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	ACTR	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
2	CREB binding protein		1.0 ~ 5.0 mM
3	TrisHCl		10 mM
4	NaCl		50 mM

Sample #2

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	ACTR	[U-90% 15N]	1.0 ~ 5.0 mM
6	CREB binding protein		1.0 ~ 5.0 mM
7	TrisHCl		10 mM
8	NaCl		50 mM

Sample #3

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
9	ACTR		1.0 ~ 5.0 mM
10	CREB binding protein	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
11	TrisHCl		10 mM
12	NaCl		50 mM

Sample #4

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	ACTR		1.0 ~ 5.0 mM
14	CREB binding protein	[U-90% 15N]	1.0 ~ 5.0 mM
15	TrisHCl		10 mM
16	NaCl		50 mM

10 2D CGCN

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DMX (600 MHz)

#4 Model Bruker AMX (600 MHz)

#5 Model Bruker DMX (750 MHz)

#6 Model Bruker DRX (800 MHz)

Sample #1

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	ACTR	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
2	CREB binding protein		1.0 ~ 5.0 mM
3	TrisHCl		10 mM
4	NaCl		50 mM

Sample #2

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	ACTR	[U-90% 15N]	1.0 ~ 5.0 mM
6	CREB binding protein		1.0 ~ 5.0 mM
7	TrisHCl		10 mM
8	NaCl		50 mM

Sample #3

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
9	ACTR		1.0 ~ 5.0 mM
10	CREB binding protein	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
11	TrisHCl		10 mM
12	NaCl		50 mM

Sample #4

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	ACTR		1.0 ~ 5.0 mM
14	CREB binding protein	[U-90% 15N]	1.0 ~ 5.0 mM
15	TrisHCl		10 mM
16	NaCl		50 mM

11 3D HACACB-COSY

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DMX (600 MHz)

#4 Model Bruker AMX (600 MHz)

#5 Model Bruker DMX (750 MHz)

#6 Model Bruker DRX (800 MHz)

Sample #1

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	ACTR	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
2	CREB binding protein		1.0 ~ 5.0 mM
3	TrisHCl		10 mM
4	NaCl		50 mM

Sample #2

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	ACTR	[U-90% 15N]	1.0 ~ 5.0 mM
6	CREB binding protein		1.0 ~ 5.0 mM
7	TrisHCl		10 mM
8	NaCl		50 mM

Sample #3

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
9	ACTR		1.0 ~ 5.0 mM
10	CREB binding protein	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
11	TrisHCl		10 mM
12	NaCl		50 mM

Sample #4

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	ACTR		1.0 ~ 5.0 mM
14	CREB binding protein	[U-90% 15N]	1.0 ~ 5.0 mM
15	TrisHCl		10 mM
16	NaCl		50 mM

12 3D HNHA

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DMX (600 MHz)

#4 Model Bruker AMX (600 MHz)

#5 Model Bruker DMX (750 MHz)

#6 Model Bruker DRX (800 MHz)

Sample #1

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	ACTR	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
2	CREB binding protein		1.0 ~ 5.0 mM
3	TrisHCl		10 mM
4	NaCl		50 mM

Sample #2

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	ACTR	[U-90% 15N]	1.0 ~ 5.0 mM
6	CREB binding protein		1.0 ~ 5.0 mM
7	TrisHCl		10 mM
8	NaCl		50 mM

Sample #3

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
9	ACTR		1.0 ~ 5.0 mM
10	CREB binding protein	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
11	TrisHCl		10 mM
12	NaCl		50 mM

Sample #4

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	ACTR		1.0 ~ 5.0 mM
14	CREB binding protein	[U-90% 15N]	1.0 ~ 5.0 mM
15	TrisHCl		10 mM
16	NaCl		50 mM

13 3D HNHB

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DMX (600 MHz)

#4 Model Bruker AMX (600 MHz)

#5 Model Bruker DMX (750 MHz)

#6 Model Bruker DRX (800 MHz)

Sample #1

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	ACTR	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
2	CREB binding protein		1.0 ~ 5.0 mM
3	TrisHCl		10 mM
4	NaCl		50 mM

Sample #2

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	ACTR	[U-90% 15N]	1.0 ~ 5.0 mM
6	CREB binding protein		1.0 ~ 5.0 mM
7	TrisHCl		10 mM
8	NaCl		50 mM

Sample #3

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
9	ACTR		1.0 ~ 5.0 mM
10	CREB binding protein	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
11	TrisHCl		10 mM
12	NaCl		50 mM

Sample #4

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	ACTR		1.0 ~ 5.0 mM
14	CREB binding protein	[U-90% 15N]	1.0 ~ 5.0 mM
15	TrisHCl		10 mM
16	NaCl		50 mM

14 3D HCCH-TOCSY

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DMX (600 MHz)

#4 Model Bruker AMX (600 MHz)

#5 Model Bruker DMX (750 MHz)

#6 Model Bruker DRX (800 MHz)

Sample #1

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	ACTR	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
2	CREB binding protein		1.0 ~ 5.0 mM
3	TrisHCl		10 mM
4	NaCl		50 mM

Sample #2

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	ACTR	[U-90% 15N]	1.0 ~ 5.0 mM
6	CREB binding protein		1.0 ~ 5.0 mM
7	TrisHCl		10 mM
8	NaCl		50 mM

Sample #3

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
9	ACTR		1.0 ~ 5.0 mM
10	CREB binding protein	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
11	TrisHCl		10 mM
12	NaCl		50 mM

Sample #4

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	ACTR		1.0 ~ 5.0 mM
14	CREB binding protein	[U-90% 15N]	1.0 ~ 5.0 mM
15	TrisHCl		10 mM
16	NaCl		50 mM

15 3D CCH-TOCSY

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DMX (600 MHz)

#4 Model Bruker AMX (600 MHz)

#5 Model Bruker DMX (750 MHz)

#6 Model Bruker DRX (800 MHz)

Sample #1

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	ACTR	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
2	CREB binding protein		1.0 ~ 5.0 mM
3	TrisHCl		10 mM
4	NaCl		50 mM

Sample #2

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	ACTR	[U-90% 15N]	1.0 ~ 5.0 mM
6	CREB binding protein		1.0 ~ 5.0 mM
7	TrisHCl		10 mM
8	NaCl		50 mM

Sample #3

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
9	ACTR		1.0 ~ 5.0 mM
10	CREB binding protein	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
11	TrisHCl		10 mM
12	NaCl		50 mM

Sample #4

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	ACTR		1.0 ~ 5.0 mM
14	CREB binding protein	[U-90% 15N]	1.0 ~ 5.0 mM
15	TrisHCl		10 mM
16	NaCl		50 mM

16 3D CCH-COSY

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DMX (600 MHz)

#4 Model Bruker AMX (600 MHz)

#5 Model Bruker DMX (750 MHz)

#6 Model Bruker DRX (800 MHz)

Sample #1

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	ACTR	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
2	CREB binding protein		1.0 ~ 5.0 mM
3	TrisHCl		10 mM
4	NaCl		50 mM

Sample #2

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	ACTR	[U-90% 15N]	1.0 ~ 5.0 mM
6	CREB binding protein		1.0 ~ 5.0 mM
7	TrisHCl		10 mM
8	NaCl		50 mM

Sample #3

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
9	ACTR		1.0 ~ 5.0 mM
10	CREB binding protein	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
11	TrisHCl		10 mM
12	NaCl		50 mM

Sample #4

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	ACTR		1.0 ~ 5.0 mM
14	CREB binding protein	[U-90% 15N]	1.0 ~ 5.0 mM
15	TrisHCl		10 mM
16	NaCl		50 mM

17 3D 1H-15N HSQC TOCSY

Instrument

#1 Model Bruker AMX (500 MHz)

#2 Model Bruker DRX (600 MHz)

#3 Model Bruker DMX (600 MHz)

#4 Model Bruker AMX (600 MHz)

#5 Model Bruker DMX (750 MHz)

#6 Model Bruker DRX (800 MHz)

Sample #1

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
1	ACTR	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
2	CREB binding protein		1.0 ~ 5.0 mM
3	TrisHCl		10 mM
4	NaCl		50 mM

Sample #2

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
5	ACTR	[U-90% 15N]	1.0 ~ 5.0 mM
6	CREB binding protein		1.0 ~ 5.0 mM
7	TrisHCl		10 mM
8	NaCl		50 mM

Sample #3

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
9	ACTR		1.0 ~ 5.0 mM
10	CREB binding protein	[U-95% 13C; U-90% 15N]	1.0 ~ 5.0 mM
11	TrisHCl		10 mM
12	NaCl		50 mM

Sample #4

Temperature 304 (±1) K, pH 6.6 (±0.1)

#	Name	Isotope labeling	Concentration
13	ACTR		1.0 ~ 5.0 mM
14	CREB binding protein	[U-90% 15N]	1.0 ~ 5.0 mM
15	TrisHCl		10 mM
16	NaCl		50 mM

Chemical shift validation 5 contents Detail

Properties

Input source #1: Assigned chemical shifts - Assigned chemical shifts	bmr5228_3.str
Diamagnetism of the molecular assembly	True (excluding Oxygen atoms)
Whether the assembly has a disulfide bond	None
Whether the assembly has a other bond	None
Whether the assembly contains a cyclic polymer	None
Overall data processing status	Warning

Disulfide bonds

None

Other bonds (neither disulfide, covalent nor hydrogen bonds, e.g. Zinc–sulphur bond)

None

Non-standard residues

None

Content subtype: bmr5228_3.str

#	Content subtype	Saveframe	Status	# of rows	Sequence coverage (%)
1	Assigned chemical shifts	ACTR_cs	OK	705	98.6 (chain: 1, length: 71)
2	Assigned chemical shifts	CBP_cs	Warning	637	96.6 (chain: 2, length: 59)
1	Chemical shift references	chemical_shift_reference	OK	3	No information

Assigned chemical shifts

Saveframe: ACTR_cs
- Status: OK
- Sequence coverage of experimental data
  - Entity_assembly_ID: 1, Chain length: 71, Sequence coverage: 98.6%
- Total number of assignments
  - ¹H chemical shifts: 378
  - ¹³C chemical shifts: 259
  - ¹⁵N chemical shifts: 68
- Completeness of assignments
  - Entity_assemble_ID: 1
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 1
Saveframe: CBP_cs
- Status: Warning
- Sequence coverage of experimental data
  - Entity_assembly_ID: 2, Chain length: 59, Sequence coverage: 96.6%
- Total number of assignments
  - ¹H chemical shifts: 365
  - ¹³C chemical shifts: 221
  - ¹⁵N chemical shifts: 51
- Completeness of assignments
  - Entity_assemble_ID: 2
- Peptide bond of PRO
- Rotameric state of ILE , LEU, and VAL
- Histogram of normalized assigned chemical shifts
- Random coil index and derived parameters (S² and NMR RMSD)
  - Chain_ID: 2

Keywords transcription, coactivator, CBP/p300, NMR assignment, p160, ACTR, nuclear hormone receptor

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	413	318	77.0
¹³C chemical shifts	298	259	86.9
¹⁵N chemical shifts	81	68	84.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	143	139	97.2
¹³C chemical shifts	142	135	95.1
¹⁵N chemical shifts	66	66	100.0

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	270	179	66.3
¹³C chemical shifts	156	124	79.5
¹⁵N chemical shifts	15	2	13.3

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	382	298	78.0
¹³C chemical shifts	265	221	83.4
¹⁵N chemical shifts	70	51	72.9

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	113	105	92.9
¹³C chemical shifts	118	100	84.7
¹⁵N chemical shifts	53	46	86.8

Atom group	# of target shifts	# of assigned shifts	Completeness (%)
¹H chemical shifts	269	193	71.7
¹³C chemical shifts	147	121	82.3
¹⁵N chemical shifts	17	5	29.4

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Found 1 Document (2.325 seconds)

BMRB: 5228

Sample #1

Sample #2

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Related entities 1. ACTR, : 2 : 25 entities Detail

Related entities 2. CREB binding protein, : 6 : 16 entities Detail

Interaction partners 1. ACTR, : 54 : 1 interactors Detail

Interaction partners 2. CREB binding protein, : 1 interactors Detail

Experiments performed 17 experiments Detail

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