Resonance assignments for stromelysin complexed with a beta-sulfonyl hydroxamate inhibitor
FRTFPGIPKW RKTHLTYRIV NYTPDLPKDA VDSAVEKALK VWEEVTPLTF SRLYEGEADI MISFAVREHG DFYPFDGPGN VLAHAYAPGP GINGDAHFDD DEQWTKDTTG TNLFLVAAHE IGHSLGLFHS ANTEALMYPL YHSLTDLTRF RLSQDDINGI QSLYGPPPDS PET
Polymer type: polypeptide(L)
Total | 1H | 13C | 15N | |
---|---|---|---|---|
All | 56.5 % (1127 of 1994) | 48.0 % (492 of 1025) | 62.2 % (496 of 798) | 81.3 % (139 of 171) |
Backbone | 82.2 % (830 of 1010) | 78.3 % (270 of 345) | 83.4 % (422 of 506) | 86.8 % (138 of 159) |
Sidechain | 38.0 % (435 of 1144) | 32.6 % (222 of 680) | 46.9 % (212 of 452) | 8.3 % (1 of 12) |
Aromatic | 2.6 % (6 of 232) | 3.4 % (4 of 116) | 1.8 % (2 of 113) | 0.0 % (0 of 3) |
Methyl | 42.9 % (78 of 182) | 47.3 % (43 of 91) | 38.5 % (35 of 91) |
1. stromelysin
FRTFPGIPKW RKTHLTYRIV NYTPDLPKDA VDSAVEKALK VWEEVTPLTF SRLYEGEADI MISFAVREHG DFYPFDGPGN VLAHAYAPGP GINGDAHFDD DEQWTKDTTG TNLFLVAAHE IGHSLGLFHS ANTEALMYPL YHSLTDLTRF RLSQDDINGI QSLYGPPPDS PETTemperature 300 (±0.1) K, pH 6.6 (±0.1)
# | Name | Isotope labeling | Type | Concentration |
---|---|---|---|---|
1 | stromelysin | [U-100% 13C; U-100% 15N] | 0.9 mM | |
2 | beta-hydroxamate inhibitor | 1 mM | ||
3 | d-imidazole | 10 mM | ||
4 | CaCl2 | 2.5 mM | ||
5 | ZnCl2 | 5 uM | ||
6 | NaN3 | 0.02 % |